Trypanosoma cruzi poly-zinc finger protein: A novel DNA/RNA-binding CCHC-zinc finger protein

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A poly-zinc finger protein, designated PZFP1 was identified in Trypanosoma cruzi for the first time. The protein has 191 amino acids, contains seven motifs Cys(X)2Cys(X)4His(X)4Cys. A recombinant PZFP1 was generated in E. coli and the expected 21kDa polypeptide co-purified with two other inducible p...

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Autores principales: Espinosa, J.M., Portal, D., Lobo, G.S., Pereira, C.A., Alonso, G.D., Gómez, E.B., Lan, G.H., Pomar, R.V.R., Flawiá, M.M., Torres, H.N.
Formato: JOUR
Materias:
CCHC-type zinc finger
Poly-zinc finger protein
Serine-arginine-rich protein
TcPZFP1
TcSR
Trypanosoma cruzi
arginine
cell extract
double stranded DNA
oligonucleotide
protein
protein antibody
protozoal protein
recombinant protein
serine
zinc finger protein
amino acid sequence
article
cell nucleus
cellular distribution
cytoplasm
Escherichia coli
gel mobility shift assay
immunofluorescence
molecular recognition
molecular weight
nonhuman
nucleotide sequence
priority journal
protein analysis
protein DNA interaction
protein induction
protein localization
protein motif
protein protein interaction
protein purification
protein RNA binding
two hybrid system
Western blotting
yeast
Trypanosoma
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_01666851_v131_n1_p35_Espinosa
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_01666851_v131_n1_p35_Espinosa
record_format dspace
spelling todo:paper_01666851_v131_n1_p35_Espinosa2023-10-03T15:04:09Z Trypanosoma cruzi poly-zinc finger protein: A novel DNA/RNA-binding CCHC-zinc finger protein Espinosa, J.M. Portal, D. Lobo, G.S. Pereira, C.A. Alonso, G.D. Gómez, E.B. Lan, G.H. Pomar, R.V.R. Flawiá, M.M. Torres, H.N. CCHC-type zinc finger Poly-zinc finger protein Serine-arginine-rich protein TcPZFP1 TcSR Trypanosoma cruzi arginine cell extract double stranded DNA oligonucleotide protein protein antibody protozoal protein recombinant protein serine zinc finger protein amino acid sequence article cell nucleus cellular distribution cytoplasm Escherichia coli gel mobility shift assay immunofluorescence molecular recognition molecular weight nonhuman nucleotide sequence priority journal protein analysis protein DNA interaction protein induction protein localization protein motif protein protein interaction protein purification protein RNA binding Trypanosoma cruzi two hybrid system Western blotting yeast Trypanosoma Trypanosoma cruzi A poly-zinc finger protein, designated PZFP1 was identified in Trypanosoma cruzi for the first time. The protein has 191 amino acids, contains seven motifs Cys(X)2Cys(X)4His(X)4Cys. A recombinant PZFP1 was generated in E. coli and the expected 21kDa polypeptide co-purified with two other inducible products of about 42 and 63kDa. Western blot analysis of cell extracts using an anti-PZFP1 antibody recognized a major band of 41kDa. Electrophoretic mobility shift analysis demonstrated that both, recombinant and native PZFP1, specifically interact with single-stranded DNA or RNA oligonucleotides carrying recognition sequences of other CCHC proteins. The protein was localized mainly in the cytoplasm and nucleus as observed by indirect immunofluorescence analysis. PZFP1 interacted specifically with a T. cruzi serine-arginine-rich protein (TcSR) in a yeast two-hybrid assay, suggesting a role in pre-mRNA processing. © 2003 Elsevier B.V. All rights reserved. Fil:Espinosa, J.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Portal, D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Lobo, G.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pereira, C.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Alonso, G.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Gómez, E.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Flawiá, M.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Torres, H.N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01666851_v131_n1_p35_Espinosa
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic CCHC-type zinc finger
Poly-zinc finger protein
Serine-arginine-rich protein
TcPZFP1
TcSR
Trypanosoma cruzi
arginine
cell extract
double stranded DNA
oligonucleotide
protein
protein antibody
protozoal protein
recombinant protein
serine
zinc finger protein
amino acid sequence
article
cell nucleus
cellular distribution
cytoplasm
Escherichia coli
gel mobility shift assay
immunofluorescence
molecular recognition
molecular weight
nonhuman
nucleotide sequence
priority journal
protein analysis
protein DNA interaction
protein induction
protein localization
protein motif
protein protein interaction
protein purification
protein RNA binding
Trypanosoma cruzi
two hybrid system
Western blotting
yeast
Trypanosoma
Trypanosoma cruzi
spellingShingle CCHC-type zinc finger
Poly-zinc finger protein
Serine-arginine-rich protein
TcPZFP1
TcSR
Trypanosoma cruzi
arginine
cell extract
double stranded DNA
oligonucleotide
protein
protein antibody
protozoal protein
recombinant protein
serine
zinc finger protein
amino acid sequence
article
cell nucleus
cellular distribution
cytoplasm
Escherichia coli
gel mobility shift assay
immunofluorescence
molecular recognition
molecular weight
nonhuman
nucleotide sequence
priority journal
protein analysis
protein DNA interaction
protein induction
protein localization
protein motif
protein protein interaction
protein purification
protein RNA binding
Trypanosoma cruzi
two hybrid system
Western blotting
yeast
Trypanosoma
Trypanosoma cruzi
Espinosa, J.M.
Portal, D.
Lobo, G.S.
Pereira, C.A.
Alonso, G.D.
Gómez, E.B.
Lan, G.H.
Pomar, R.V.R.
Flawiá, M.M.
Torres, H.N.
Trypanosoma cruzi poly-zinc finger protein: A novel DNA/RNA-binding CCHC-zinc finger protein
topic_facet CCHC-type zinc finger
Poly-zinc finger protein
Serine-arginine-rich protein
TcPZFP1
TcSR
Trypanosoma cruzi
arginine
cell extract
double stranded DNA
oligonucleotide
protein
protein antibody
protozoal protein
recombinant protein
serine
zinc finger protein
amino acid sequence
article
cell nucleus
cellular distribution
cytoplasm
Escherichia coli
gel mobility shift assay
immunofluorescence
molecular recognition
molecular weight
nonhuman
nucleotide sequence
priority journal
protein analysis
protein DNA interaction
protein induction
protein localization
protein motif
protein protein interaction
protein purification
protein RNA binding
Trypanosoma cruzi
two hybrid system
Western blotting
yeast
Trypanosoma
Trypanosoma cruzi
description A poly-zinc finger protein, designated PZFP1 was identified in Trypanosoma cruzi for the first time. The protein has 191 amino acids, contains seven motifs Cys(X)2Cys(X)4His(X)4Cys. A recombinant PZFP1 was generated in E. coli and the expected 21kDa polypeptide co-purified with two other inducible products of about 42 and 63kDa. Western blot analysis of cell extracts using an anti-PZFP1 antibody recognized a major band of 41kDa. Electrophoretic mobility shift analysis demonstrated that both, recombinant and native PZFP1, specifically interact with single-stranded DNA or RNA oligonucleotides carrying recognition sequences of other CCHC proteins. The protein was localized mainly in the cytoplasm and nucleus as observed by indirect immunofluorescence analysis. PZFP1 interacted specifically with a T. cruzi serine-arginine-rich protein (TcSR) in a yeast two-hybrid assay, suggesting a role in pre-mRNA processing. © 2003 Elsevier B.V. All rights reserved.
format JOUR
author Espinosa, J.M.
Portal, D.
Lobo, G.S.
Pereira, C.A.
Alonso, G.D.
Gómez, E.B.
Lan, G.H.
Pomar, R.V.R.
Flawiá, M.M.
Torres, H.N.
author_facet Espinosa, J.M.
Portal, D.
Lobo, G.S.
Pereira, C.A.
Alonso, G.D.
Gómez, E.B.
Lan, G.H.
Pomar, R.V.R.
Flawiá, M.M.
Torres, H.N.
author_sort Espinosa, J.M.
title Trypanosoma cruzi poly-zinc finger protein: A novel DNA/RNA-binding CCHC-zinc finger protein
title_short Trypanosoma cruzi poly-zinc finger protein: A novel DNA/RNA-binding CCHC-zinc finger protein
title_full Trypanosoma cruzi poly-zinc finger protein: A novel DNA/RNA-binding CCHC-zinc finger protein
title_fullStr Trypanosoma cruzi poly-zinc finger protein: A novel DNA/RNA-binding CCHC-zinc finger protein
title_full_unstemmed Trypanosoma cruzi poly-zinc finger protein: A novel DNA/RNA-binding CCHC-zinc finger protein
title_sort trypanosoma cruzi poly-zinc finger protein: a novel dna/rna-binding cchc-zinc finger protein
url http://hdl.handle.net/20.500.12110/paper_01666851_v131_n1_p35_Espinosa
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