Protective effect of a natural carrageenan on genital herpes simplex virus infection in mice

In the present study, the protective effect of 1T1, a λ-carrageenan extracted from the red seaweed Gigartina skottsbergii was evaluated in a murine model of herpes simplex virus type 2 (HSV-2) genital infection. Six to eight-week-old female BALB/c mice were intravaginally inoculated with a lethal do...

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Autores principales:	Carlucci, M.J., Scolaro, L.A., Noseda, M.D., Cerezo, A.S., Damonte, E.B.
Formato:	JOUR
Materias:	Herpes simplex virus type 2 Natural carrageenan Sexually transmitted disease Vaginal infection Virucidal activity carrageenan animal experiment animal model article controlled study dose response drug effect drug isolation drug mechanism female genital herpes Herpes simplex virus 2 infection prevention inoculation lethal dose mortality mouse nonhuman priority journal seaweed survival rate time vagina secretion virus replication virus shedding virus strain Animals Antiviral Agents Carrageenan Cercopithecus aethiops Female Herpes Genitalis Herpesvirus 2, Human Mice Mice, Inbred BALB C Vagina Vaginal Diseases Vero Cells Virus Shedding algae Gigartina Gigartina skottsbergii Herpesviridae Human herpesvirus 2 Murinae Simplexvirus
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_01663542_v64_n2_p137_Carlucci
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	todo:paper_01663542_v64_n2_p137_Carlucci2023-10-03T15:03:51Z Protective effect of a natural carrageenan on genital herpes simplex virus infection in mice Carlucci, M.J. Scolaro, L.A. Noseda, M.D. Cerezo, A.S. Damonte, E.B. Herpes simplex virus type 2 Natural carrageenan Sexually transmitted disease Vaginal infection Virucidal activity carrageenan animal experiment animal model article controlled study dose response drug effect drug isolation drug mechanism female genital herpes Herpes simplex virus 2 infection prevention inoculation lethal dose mortality mouse nonhuman priority journal seaweed survival rate time vagina secretion virus replication virus shedding virus strain Animals Antiviral Agents Carrageenan Cercopithecus aethiops Female Herpes Genitalis Herpesvirus 2, Human Mice Mice, Inbred BALB C Vagina Vaginal Diseases Vero Cells Virus Shedding algae Gigartina Gigartina skottsbergii Herpesviridae Human herpesvirus 2 Murinae Simplexvirus In the present study, the protective effect of 1T1, a λ-carrageenan extracted from the red seaweed Gigartina skottsbergii was evaluated in a murine model of herpes simplex virus type 2 (HSV-2) genital infection. Six to eight-week-old female BALB/c mice were intravaginally inoculated with a lethal dose of HSV-2 (MS strain) and pre- or post-infection treated with different doses of a 10 mg/ml solution of 1T1. A single topical administration of 1T1 shortly before infection of BALB/c mice with HSV-2 protected 9 out of 10 mice from HSV-2-induced lesions and mortality, compared with only 10% survival in control mice. In addition, 1T1 produced a total blockade in virus shedding in the vaginal secretions. When 1T1 pre-treatment was reinforced with a second dose 2 h after infection, total protection was observed even when the prophylactic administration had taken place at 60 min before infection. The irreversible virucidal action of 1T1 against herpes virus seems to be responsible of its protective effect against virus replication and mortality following vaginal HSV-2 infection. © 2004 Elsevier B.V. All rights reserved. Fil:Carlucci, M.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Scolaro, L.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Noseda, M.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Cerezo, A.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Damonte, E.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01663542_v64_n2_p137_Carlucci
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Herpes simplex virus type 2 Natural carrageenan Sexually transmitted disease Vaginal infection Virucidal activity carrageenan animal experiment animal model article controlled study dose response drug effect drug isolation drug mechanism female genital herpes Herpes simplex virus 2 infection prevention inoculation lethal dose mortality mouse nonhuman priority journal seaweed survival rate time vagina secretion virus replication virus shedding virus strain Animals Antiviral Agents Carrageenan Cercopithecus aethiops Female Herpes Genitalis Herpesvirus 2, Human Mice Mice, Inbred BALB C Vagina Vaginal Diseases Vero Cells Virus Shedding algae Gigartina Gigartina skottsbergii Herpesviridae Human herpesvirus 2 Murinae Simplexvirus
spellingShingle	Herpes simplex virus type 2 Natural carrageenan Sexually transmitted disease Vaginal infection Virucidal activity carrageenan animal experiment animal model article controlled study dose response drug effect drug isolation drug mechanism female genital herpes Herpes simplex virus 2 infection prevention inoculation lethal dose mortality mouse nonhuman priority journal seaweed survival rate time vagina secretion virus replication virus shedding virus strain Animals Antiviral Agents Carrageenan Cercopithecus aethiops Female Herpes Genitalis Herpesvirus 2, Human Mice Mice, Inbred BALB C Vagina Vaginal Diseases Vero Cells Virus Shedding algae Gigartina Gigartina skottsbergii Herpesviridae Human herpesvirus 2 Murinae Simplexvirus Carlucci, M.J. Scolaro, L.A. Noseda, M.D. Cerezo, A.S. Damonte, E.B. Protective effect of a natural carrageenan on genital herpes simplex virus infection in mice
topic_facet	Herpes simplex virus type 2 Natural carrageenan Sexually transmitted disease Vaginal infection Virucidal activity carrageenan animal experiment animal model article controlled study dose response drug effect drug isolation drug mechanism female genital herpes Herpes simplex virus 2 infection prevention inoculation lethal dose mortality mouse nonhuman priority journal seaweed survival rate time vagina secretion virus replication virus shedding virus strain Animals Antiviral Agents Carrageenan Cercopithecus aethiops Female Herpes Genitalis Herpesvirus 2, Human Mice Mice, Inbred BALB C Vagina Vaginal Diseases Vero Cells Virus Shedding algae Gigartina Gigartina skottsbergii Herpesviridae Human herpesvirus 2 Murinae Simplexvirus
description	In the present study, the protective effect of 1T1, a λ-carrageenan extracted from the red seaweed Gigartina skottsbergii was evaluated in a murine model of herpes simplex virus type 2 (HSV-2) genital infection. Six to eight-week-old female BALB/c mice were intravaginally inoculated with a lethal dose of HSV-2 (MS strain) and pre- or post-infection treated with different doses of a 10 mg/ml solution of 1T1. A single topical administration of 1T1 shortly before infection of BALB/c mice with HSV-2 protected 9 out of 10 mice from HSV-2-induced lesions and mortality, compared with only 10% survival in control mice. In addition, 1T1 produced a total blockade in virus shedding in the vaginal secretions. When 1T1 pre-treatment was reinforced with a second dose 2 h after infection, total protection was observed even when the prophylactic administration had taken place at 60 min before infection. The irreversible virucidal action of 1T1 against herpes virus seems to be responsible of its protective effect against virus replication and mortality following vaginal HSV-2 infection. © 2004 Elsevier B.V. All rights reserved.
format	JOUR
author	Carlucci, M.J. Scolaro, L.A. Noseda, M.D. Cerezo, A.S. Damonte, E.B.
author_facet	Carlucci, M.J. Scolaro, L.A. Noseda, M.D. Cerezo, A.S. Damonte, E.B.
author_sort	Carlucci, M.J.
title	Protective effect of a natural carrageenan on genital herpes simplex virus infection in mice
title_short	Protective effect of a natural carrageenan on genital herpes simplex virus infection in mice
title_full	Protective effect of a natural carrageenan on genital herpes simplex virus infection in mice
title_fullStr	Protective effect of a natural carrageenan on genital herpes simplex virus infection in mice
title_full_unstemmed	Protective effect of a natural carrageenan on genital herpes simplex virus infection in mice
title_sort	protective effect of a natural carrageenan on genital herpes simplex virus infection in mice
url	http://hdl.handle.net/20.500.12110/paper_01663542_v64_n2_p137_Carlucci
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Protective effect of a natural carrageenan on genital herpes simplex virus infection in mice

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