A simple method to eliminate mycoplasma from cell cultures
The efficacy of several antibiotic treatments to eliminate mycoplasma from Vero cells contaminated chronically with Mycoplasma orale II were tested. Minocyclin, Kanamycin, Tylosine and Roxitromycin, at non cytotoxic concentrations, were assayed alone or in different combinations. Mycoplasma contamin...
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todo:paper_01660934_v46_n1_p85_Coronato2023-10-03T15:03:10Z A simple method to eliminate mycoplasma from cell cultures Coronato, S. Vullo, D. Coto, C.E. Antibiotic treatment Cell lines Contaminated cells Mycoplasma antibiotic agent kanamycin minocycline roxithromycin tylosin animal cell article cell culture contamination drug efficacy mycoplasma nonhuman priority journal Animal Antibiotics, Combined Cells, Cultured Cercopithecus aethiops Drug Resistance, Microbial Eukaryotic Cells Hamsters Human Kanamycin Minocycline Mycoplasma Roxithromycin Tissue Culture Tumor Cells, Cultured Tylosin Animalia Eukaryota Mycoplasma Mycoplasma orale The efficacy of several antibiotic treatments to eliminate mycoplasma from Vero cells contaminated chronically with Mycoplasma orale II were tested. Minocyclin, Kanamycin, Tylosine and Roxitromycin, at non cytotoxic concentrations, were assayed alone or in different combinations. Mycoplasma contamination was effectively eradicated without recurrence once the following regime was applied: Incubation of contaminated cells with Tylosine (250 μg/ml) for 12 days followed by incubation with Minocycline (5 μg/ml) for 10 days. This treatment was not deletereus for cell growth, it was effective after only one application and it was successful to eradicate mycoplasma from other contaminated eukaryotic continuous cell lines. © 1994. Fil:Coto, C.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01660934_v46_n1_p85_Coronato |
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Universidad de Buenos Aires |
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I-28 |
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R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Antibiotic treatment Cell lines Contaminated cells Mycoplasma antibiotic agent kanamycin minocycline roxithromycin tylosin animal cell article cell culture contamination drug efficacy mycoplasma nonhuman priority journal Animal Antibiotics, Combined Cells, Cultured Cercopithecus aethiops Drug Resistance, Microbial Eukaryotic Cells Hamsters Human Kanamycin Minocycline Mycoplasma Roxithromycin Tissue Culture Tumor Cells, Cultured Tylosin Animalia Eukaryota Mycoplasma Mycoplasma orale |
| spellingShingle |
Antibiotic treatment Cell lines Contaminated cells Mycoplasma antibiotic agent kanamycin minocycline roxithromycin tylosin animal cell article cell culture contamination drug efficacy mycoplasma nonhuman priority journal Animal Antibiotics, Combined Cells, Cultured Cercopithecus aethiops Drug Resistance, Microbial Eukaryotic Cells Hamsters Human Kanamycin Minocycline Mycoplasma Roxithromycin Tissue Culture Tumor Cells, Cultured Tylosin Animalia Eukaryota Mycoplasma Mycoplasma orale Coronato, S. Vullo, D. Coto, C.E. A simple method to eliminate mycoplasma from cell cultures |
| topic_facet |
Antibiotic treatment Cell lines Contaminated cells Mycoplasma antibiotic agent kanamycin minocycline roxithromycin tylosin animal cell article cell culture contamination drug efficacy mycoplasma nonhuman priority journal Animal Antibiotics, Combined Cells, Cultured Cercopithecus aethiops Drug Resistance, Microbial Eukaryotic Cells Hamsters Human Kanamycin Minocycline Mycoplasma Roxithromycin Tissue Culture Tumor Cells, Cultured Tylosin Animalia Eukaryota Mycoplasma Mycoplasma orale |
| description |
The efficacy of several antibiotic treatments to eliminate mycoplasma from Vero cells contaminated chronically with Mycoplasma orale II were tested. Minocyclin, Kanamycin, Tylosine and Roxitromycin, at non cytotoxic concentrations, were assayed alone or in different combinations. Mycoplasma contamination was effectively eradicated without recurrence once the following regime was applied: Incubation of contaminated cells with Tylosine (250 μg/ml) for 12 days followed by incubation with Minocycline (5 μg/ml) for 10 days. This treatment was not deletereus for cell growth, it was effective after only one application and it was successful to eradicate mycoplasma from other contaminated eukaryotic continuous cell lines. © 1994. |
| format |
JOUR |
| author |
Coronato, S. Vullo, D. Coto, C.E. |
| author_facet |
Coronato, S. Vullo, D. Coto, C.E. |
| author_sort |
Coronato, S. |
| title |
A simple method to eliminate mycoplasma from cell cultures |
| title_short |
A simple method to eliminate mycoplasma from cell cultures |
| title_full |
A simple method to eliminate mycoplasma from cell cultures |
| title_fullStr |
A simple method to eliminate mycoplasma from cell cultures |
| title_full_unstemmed |
A simple method to eliminate mycoplasma from cell cultures |
| title_sort |
simple method to eliminate mycoplasma from cell cultures |
| url |
http://hdl.handle.net/20.500.12110/paper_01660934_v46_n1_p85_Coronato |
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