T cell vaccination in secondary progressive multiple sclerosis
Four secondary progressive MS patients were vaccinated with bovine myelin-reactive irradiated T cell lines from their peripheral blood. Patients were followed for 30-39 months, and monitored for immunological responses toward the vaccine, and for their clinical characteristics. Two patients showed s...
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todo:paper_01655728_v107_n2_p130_Correale2023-10-03T15:02:56Z T cell vaccination in secondary progressive multiple sclerosis Correale, J. Lund, B. McMillan, M. Ko, D.Y. McCarthy, K. Weiner, L.P. Lymphocytes Multiple sclerosis Myelin proteins Vaccination antibody specificity antigen specificity autoimmunity conference paper gene targeting human human cell immune response major histocompatibility complex restriction molecular cloning multiple sclerosis oligodendroglia priority journal T lymphocyte activation vaccination Animals Antibodies, Monoclonal Antibody Formation Cattle CD8-Positive T-Lymphocytes Cell Line Disability Evaluation Fas Ligand Protein Humans Interferon Type II Membrane Glycoproteins Multiple Sclerosis, Chronic Progressive Myelin Proteins Pilot Projects Receptors, Antigen, T-Cell, alpha-beta Species Specificity Tumor Necrosis Factor-alpha Vaccination Four secondary progressive MS patients were vaccinated with bovine myelin-reactive irradiated T cell lines from their peripheral blood. Patients were followed for 30-39 months, and monitored for immunological responses toward the vaccine, and for their clinical characteristics. Two patients showed stable EDSS score over time, one patient showed improvement by one EDSS step, and in the remaining patient her EDSS advanced over time. After the second inoculation there was a progressive decline of circulating whole myelin-reactive T cells, MBP143-168, PLP104-117, and MOG43-55-peptide- reactive T cells. In contrast the frequency of tetanus toxoid-reactive T cells remained unchanged. T cell vaccination (TCV) was also associated with a decline of myelin-specific IL-2- and IFN-γ-secreting T cells. Twelve T cell lines (TCL) that recognize the inoculates were isolated from the peripheral blood of two patients. Ten of these TCL were CD8+ and lysed the inoculates in a MHC Class I restricted manner. The remaining two TCL were CD4+, and lysed the inoculates by MHC Class II restricted cytolytic activity. All T cell lines lysed not only myelin-reactive T cells, but also TCL specific for MBP143-168, PLP104-117 and MOG43-55 peptides. Control TCL specific for tetanus toxoid were not lysed. Neutralizing anti-Fas mAb did not influence the killing. Moreover, culture supernatants from two TCL which produce IL-10, were able to block the proliferation of myelin protein-specific TCL. This effect was abrogated using mAbs specific for IL-10. The data obtained indicated that TCV using autologous irradiated bovine myelin-reactive T cells promotes an effective depletion of T cells reactive against different myelin antigens. (C) 2000 Elsevier Science B.V. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01655728_v107_n2_p130_Correale |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Lymphocytes Multiple sclerosis Myelin proteins Vaccination antibody specificity antigen specificity autoimmunity conference paper gene targeting human human cell immune response major histocompatibility complex restriction molecular cloning multiple sclerosis oligodendroglia priority journal T lymphocyte activation vaccination Animals Antibodies, Monoclonal Antibody Formation Cattle CD8-Positive T-Lymphocytes Cell Line Disability Evaluation Fas Ligand Protein Humans Interferon Type II Membrane Glycoproteins Multiple Sclerosis, Chronic Progressive Myelin Proteins Pilot Projects Receptors, Antigen, T-Cell, alpha-beta Species Specificity Tumor Necrosis Factor-alpha Vaccination |
| spellingShingle |
Lymphocytes Multiple sclerosis Myelin proteins Vaccination antibody specificity antigen specificity autoimmunity conference paper gene targeting human human cell immune response major histocompatibility complex restriction molecular cloning multiple sclerosis oligodendroglia priority journal T lymphocyte activation vaccination Animals Antibodies, Monoclonal Antibody Formation Cattle CD8-Positive T-Lymphocytes Cell Line Disability Evaluation Fas Ligand Protein Humans Interferon Type II Membrane Glycoproteins Multiple Sclerosis, Chronic Progressive Myelin Proteins Pilot Projects Receptors, Antigen, T-Cell, alpha-beta Species Specificity Tumor Necrosis Factor-alpha Vaccination Correale, J. Lund, B. McMillan, M. Ko, D.Y. McCarthy, K. Weiner, L.P. T cell vaccination in secondary progressive multiple sclerosis |
| topic_facet |
Lymphocytes Multiple sclerosis Myelin proteins Vaccination antibody specificity antigen specificity autoimmunity conference paper gene targeting human human cell immune response major histocompatibility complex restriction molecular cloning multiple sclerosis oligodendroglia priority journal T lymphocyte activation vaccination Animals Antibodies, Monoclonal Antibody Formation Cattle CD8-Positive T-Lymphocytes Cell Line Disability Evaluation Fas Ligand Protein Humans Interferon Type II Membrane Glycoproteins Multiple Sclerosis, Chronic Progressive Myelin Proteins Pilot Projects Receptors, Antigen, T-Cell, alpha-beta Species Specificity Tumor Necrosis Factor-alpha Vaccination |
| description |
Four secondary progressive MS patients were vaccinated with bovine myelin-reactive irradiated T cell lines from their peripheral blood. Patients were followed for 30-39 months, and monitored for immunological responses toward the vaccine, and for their clinical characteristics. Two patients showed stable EDSS score over time, one patient showed improvement by one EDSS step, and in the remaining patient her EDSS advanced over time. After the second inoculation there was a progressive decline of circulating whole myelin-reactive T cells, MBP143-168, PLP104-117, and MOG43-55-peptide- reactive T cells. In contrast the frequency of tetanus toxoid-reactive T cells remained unchanged. T cell vaccination (TCV) was also associated with a decline of myelin-specific IL-2- and IFN-γ-secreting T cells. Twelve T cell lines (TCL) that recognize the inoculates were isolated from the peripheral blood of two patients. Ten of these TCL were CD8+ and lysed the inoculates in a MHC Class I restricted manner. The remaining two TCL were CD4+, and lysed the inoculates by MHC Class II restricted cytolytic activity. All T cell lines lysed not only myelin-reactive T cells, but also TCL specific for MBP143-168, PLP104-117 and MOG43-55 peptides. Control TCL specific for tetanus toxoid were not lysed. Neutralizing anti-Fas mAb did not influence the killing. Moreover, culture supernatants from two TCL which produce IL-10, were able to block the proliferation of myelin protein-specific TCL. This effect was abrogated using mAbs specific for IL-10. The data obtained indicated that TCV using autologous irradiated bovine myelin-reactive T cells promotes an effective depletion of T cells reactive against different myelin antigens. (C) 2000 Elsevier Science B.V. |
| format |
JOUR |
| author |
Correale, J. Lund, B. McMillan, M. Ko, D.Y. McCarthy, K. Weiner, L.P. |
| author_facet |
Correale, J. Lund, B. McMillan, M. Ko, D.Y. McCarthy, K. Weiner, L.P. |
| author_sort |
Correale, J. |
| title |
T cell vaccination in secondary progressive multiple sclerosis |
| title_short |
T cell vaccination in secondary progressive multiple sclerosis |
| title_full |
T cell vaccination in secondary progressive multiple sclerosis |
| title_fullStr |
T cell vaccination in secondary progressive multiple sclerosis |
| title_full_unstemmed |
T cell vaccination in secondary progressive multiple sclerosis |
| title_sort |
t cell vaccination in secondary progressive multiple sclerosis |
| url |
http://hdl.handle.net/20.500.12110/paper_01655728_v107_n2_p130_Correale |
| work_keys_str_mv |
AT correalej tcellvaccinationinsecondaryprogressivemultiplesclerosis AT lundb tcellvaccinationinsecondaryprogressivemultiplesclerosis AT mcmillanm tcellvaccinationinsecondaryprogressivemultiplesclerosis AT kody tcellvaccinationinsecondaryprogressivemultiplesclerosis AT mccarthyk tcellvaccinationinsecondaryprogressivemultiplesclerosis AT weinerlp tcellvaccinationinsecondaryprogressivemultiplesclerosis |
| _version_ |
1807316842194141184 |