Ontogenesis of [3H]serotonin binding sites in the hypothalamus of the female rat: Relation to serotonin-induced LH release in moxestrol-pretreated rats
The release of luteinizing hormone (LH) evoked by exogenous serotonin (5 mg/kg, i.p.) was measured at different developmental ages in the female rat. Since LH response to serotonin is modulated by estrogen, moxestrol, a synthetic compound which does not bind to α-fetoprotein, was administered 48 h b...
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todo:paper_01653806_v25_n1_p111_BecuVillalobos2023-10-03T15:02:55Z Ontogenesis of [3H]serotonin binding sites in the hypothalamus of the female rat: Relation to serotonin-induced LH release in moxestrol-pretreated rats Becú-Villalobos, D. Libertun, C. hypothalamic-preoptic suprachiasmatic area luteinizing hormone (LH) moxestrol ontogenesis prolactin [3H]serotonin binding indoleamine moxestrol prolactin radioisotope animal experiment autonomic nervous system autopsy central nervous system drug binding drug receptor binding etiology hypothalamus intraperitoneal drug administration luteinizing hormone release methodology nonhuman pharmacokinetics preoptic area priority journal rat serotonin h 3 subcutaneous drug administration suprachiasmatic nucleus The release of luteinizing hormone (LH) evoked by exogenous serotonin (5 mg/kg, i.p.) was measured at different developmental ages in the female rat. Since LH response to serotonin is modulated by estrogen, moxestrol, a synthetic compound which does not bind to α-fetoprotein, was administered 48 h before serotonin or saline injections. Serotonin was ineffective in releasing LH in 5-day females pretreated with moxestrol; the response increased abruptly at 12 and 18 days and from then onwards decreased gradually. This is in clear contrast to the development of the serotonin-induced prolactin release, which is absent in the first postnatal week, becomes evident on day 12 and increases gradually as the rat approaches puberty. In a second group of experiments, the ontogenesis of hypothalamic-preoptic suprachiasmatic [3H]serotonin binding was studied in 5-, 16-, 27- and 37-day-old female rats. A specific serotonergic binding site could be quantified at 5 days (Bmax 4.31 : 1.89 fmol/μg DNA; Kd:2.74 : 1.17 nM). A gradual increase of both, Bmax and Kd was observed as the animal matured: the number of binding sites almost doubled from day 16 to day 27, and increased even further by 37 days (Bmax 24.21 : 5.16 fmol/μg DNA). These data indicate: (a) a specific [3H]serotonin binding site in hypothalamic-preoptic suprachiasmatic area is present in the first postnatal week of the female rat: (b) Bmax and Kd values increase from 5 to 37 days of age; (c) serotonin-induced LH release in moxestrol-pretreated females is maximal in postnatal weeks 2-3; and (d) ontogenesis of serotonin binding sites in the hypothalamus is in correlation to prolactin, but not to LH release evoked by exogenously administered indoleamine. © 1986. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01653806_v25_n1_p111_BecuVillalobos |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
hypothalamic-preoptic suprachiasmatic area luteinizing hormone (LH) moxestrol ontogenesis prolactin [3H]serotonin binding indoleamine moxestrol prolactin radioisotope animal experiment autonomic nervous system autopsy central nervous system drug binding drug receptor binding etiology hypothalamus intraperitoneal drug administration luteinizing hormone release methodology nonhuman pharmacokinetics preoptic area priority journal rat serotonin h 3 subcutaneous drug administration suprachiasmatic nucleus |
| spellingShingle |
hypothalamic-preoptic suprachiasmatic area luteinizing hormone (LH) moxestrol ontogenesis prolactin [3H]serotonin binding indoleamine moxestrol prolactin radioisotope animal experiment autonomic nervous system autopsy central nervous system drug binding drug receptor binding etiology hypothalamus intraperitoneal drug administration luteinizing hormone release methodology nonhuman pharmacokinetics preoptic area priority journal rat serotonin h 3 subcutaneous drug administration suprachiasmatic nucleus Becú-Villalobos, D. Libertun, C. Ontogenesis of [3H]serotonin binding sites in the hypothalamus of the female rat: Relation to serotonin-induced LH release in moxestrol-pretreated rats |
| topic_facet |
hypothalamic-preoptic suprachiasmatic area luteinizing hormone (LH) moxestrol ontogenesis prolactin [3H]serotonin binding indoleamine moxestrol prolactin radioisotope animal experiment autonomic nervous system autopsy central nervous system drug binding drug receptor binding etiology hypothalamus intraperitoneal drug administration luteinizing hormone release methodology nonhuman pharmacokinetics preoptic area priority journal rat serotonin h 3 subcutaneous drug administration suprachiasmatic nucleus |
| description |
The release of luteinizing hormone (LH) evoked by exogenous serotonin (5 mg/kg, i.p.) was measured at different developmental ages in the female rat. Since LH response to serotonin is modulated by estrogen, moxestrol, a synthetic compound which does not bind to α-fetoprotein, was administered 48 h before serotonin or saline injections. Serotonin was ineffective in releasing LH in 5-day females pretreated with moxestrol; the response increased abruptly at 12 and 18 days and from then onwards decreased gradually. This is in clear contrast to the development of the serotonin-induced prolactin release, which is absent in the first postnatal week, becomes evident on day 12 and increases gradually as the rat approaches puberty. In a second group of experiments, the ontogenesis of hypothalamic-preoptic suprachiasmatic [3H]serotonin binding was studied in 5-, 16-, 27- and 37-day-old female rats. A specific serotonergic binding site could be quantified at 5 days (Bmax 4.31 : 1.89 fmol/μg DNA; Kd:2.74 : 1.17 nM). A gradual increase of both, Bmax and Kd was observed as the animal matured: the number of binding sites almost doubled from day 16 to day 27, and increased even further by 37 days (Bmax 24.21 : 5.16 fmol/μg DNA). These data indicate: (a) a specific [3H]serotonin binding site in hypothalamic-preoptic suprachiasmatic area is present in the first postnatal week of the female rat: (b) Bmax and Kd values increase from 5 to 37 days of age; (c) serotonin-induced LH release in moxestrol-pretreated females is maximal in postnatal weeks 2-3; and (d) ontogenesis of serotonin binding sites in the hypothalamus is in correlation to prolactin, but not to LH release evoked by exogenously administered indoleamine. © 1986. |
| format |
JOUR |
| author |
Becú-Villalobos, D. Libertun, C. |
| author_facet |
Becú-Villalobos, D. Libertun, C. |
| author_sort |
Becú-Villalobos, D. |
| title |
Ontogenesis of [3H]serotonin binding sites in the hypothalamus of the female rat: Relation to serotonin-induced LH release in moxestrol-pretreated rats |
| title_short |
Ontogenesis of [3H]serotonin binding sites in the hypothalamus of the female rat: Relation to serotonin-induced LH release in moxestrol-pretreated rats |
| title_full |
Ontogenesis of [3H]serotonin binding sites in the hypothalamus of the female rat: Relation to serotonin-induced LH release in moxestrol-pretreated rats |
| title_fullStr |
Ontogenesis of [3H]serotonin binding sites in the hypothalamus of the female rat: Relation to serotonin-induced LH release in moxestrol-pretreated rats |
| title_full_unstemmed |
Ontogenesis of [3H]serotonin binding sites in the hypothalamus of the female rat: Relation to serotonin-induced LH release in moxestrol-pretreated rats |
| title_sort |
ontogenesis of [3h]serotonin binding sites in the hypothalamus of the female rat: relation to serotonin-induced lh release in moxestrol-pretreated rats |
| url |
http://hdl.handle.net/20.500.12110/paper_01653806_v25_n1_p111_BecuVillalobos |
| work_keys_str_mv |
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