Cellular immunity in chagas' disease patients. Lymphoproliferative response to subcellular fractions of Trypanosoma cruzi

The ability of peripheral mononuclear cells from Chagas' disease patients (CDP) to generate specific proliferative responses in vitro to whole homogenate (WH) and subcellular fractions of T. cruzi was investigated. Flagellar (F), microsomal (Mc) and cell sap (CS) fractions were isolated from a...

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Autores principales: De Titto, E.H., Segura, E.L., Braun, M.
Formato: JOUR
Materias:
cellular immunity
Chagas' disease
lymphoproliferative response
specific response in Chagas' disease
subcellular fractions of T. cruzi
Trypanosoma cruzi
blood and hemopoietic system
chagas disease
clinical article
diagnosis
etiology
human
protozoon
trypanosoma cruzi
Cell Count
Cell Separation
Chagas Disease
Human
Immunity, Cellular
Kinetics
Lymphocyte Activation
Lymphocytes
Phytohemagglutinins
Subcellular Fractions
Support, Non-U.S. Gov't
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_01652478_v6_n3_p161_DeTitto
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_01652478_v6_n3_p161_DeTitto
record_format dspace
spelling todo:paper_01652478_v6_n3_p161_DeTitto2023-10-03T15:02:54Z Cellular immunity in chagas' disease patients. Lymphoproliferative response to subcellular fractions of Trypanosoma cruzi De Titto, E.H. Segura, E.L. Braun, M. cellular immunity Chagas' disease lymphoproliferative response specific response in Chagas' disease subcellular fractions of T. cruzi Trypanosoma cruzi blood and hemopoietic system cellular immunity chagas disease clinical article diagnosis etiology human protozoon trypanosoma cruzi Cell Count Cell Separation Chagas Disease Human Immunity, Cellular Kinetics Lymphocyte Activation Lymphocytes Phytohemagglutinins Subcellular Fractions Support, Non-U.S. Gov't Trypanosoma cruzi The ability of peripheral mononuclear cells from Chagas' disease patients (CDP) to generate specific proliferative responses in vitro to whole homogenate (WH) and subcellular fractions of T. cruzi was investigated. Flagellar (F), microsomal (Mc) and cell sap (CS) fractions were isolated from a WH obtained by disruption by pressure-depressure. Parasite-free human peripheral mononuclear cells were isolated by centrifugation of leukocyte-rich plasma over discontinuous gradients of Ficoll-Hypaque and cultured for 6 days in the presence of the different fractions of T. cruzi. Eighty-six percent ( 54 63) of the CDP showed good capacity to respond to at least one of the fractions assayed while only 18% ( 4 22) of the controls reacted. The Mc fraction showed the best specificity as well as the maximal stimulating effect in CDP ( 37 55 vs 0 18 in controls) while WH (62% vs 10%), F (54% vs 16%) and CS (52% vs 6%) showed a lesser antigenic capacity. These results suggest that recognition mechanisms and proliferative responses are functional in CDP. No correlation was found between the stimulating capacity of fractions in vitro and their immunogenic capacity previously observed in vivo. © 1983. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01652478_v6_n3_p161_DeTitto
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic cellular immunity
Chagas' disease
lymphoproliferative response
specific response in Chagas' disease
subcellular fractions of T. cruzi
Trypanosoma cruzi
blood and hemopoietic system
cellular immunity
chagas disease
clinical article
diagnosis
etiology
human
protozoon
trypanosoma cruzi
Cell Count
Cell Separation
Chagas Disease
Human
Immunity, Cellular
Kinetics
Lymphocyte Activation
Lymphocytes
Phytohemagglutinins
Subcellular Fractions
Support, Non-U.S. Gov't
Trypanosoma cruzi
spellingShingle cellular immunity
Chagas' disease
lymphoproliferative response
specific response in Chagas' disease
subcellular fractions of T. cruzi
Trypanosoma cruzi
blood and hemopoietic system
cellular immunity
chagas disease
clinical article
diagnosis
etiology
human
protozoon
trypanosoma cruzi
Cell Count
Cell Separation
Chagas Disease
Human
Immunity, Cellular
Kinetics
Lymphocyte Activation
Lymphocytes
Phytohemagglutinins
Subcellular Fractions
Support, Non-U.S. Gov't
Trypanosoma cruzi
De Titto, E.H.
Segura, E.L.
Braun, M.
Cellular immunity in chagas' disease patients. Lymphoproliferative response to subcellular fractions of Trypanosoma cruzi
topic_facet cellular immunity
Chagas' disease
lymphoproliferative response
specific response in Chagas' disease
subcellular fractions of T. cruzi
Trypanosoma cruzi
blood and hemopoietic system
cellular immunity
chagas disease
clinical article
diagnosis
etiology
human
protozoon
trypanosoma cruzi
Cell Count
Cell Separation
Chagas Disease
Human
Immunity, Cellular
Kinetics
Lymphocyte Activation
Lymphocytes
Phytohemagglutinins
Subcellular Fractions
Support, Non-U.S. Gov't
Trypanosoma cruzi
description The ability of peripheral mononuclear cells from Chagas' disease patients (CDP) to generate specific proliferative responses in vitro to whole homogenate (WH) and subcellular fractions of T. cruzi was investigated. Flagellar (F), microsomal (Mc) and cell sap (CS) fractions were isolated from a WH obtained by disruption by pressure-depressure. Parasite-free human peripheral mononuclear cells were isolated by centrifugation of leukocyte-rich plasma over discontinuous gradients of Ficoll-Hypaque and cultured for 6 days in the presence of the different fractions of T. cruzi. Eighty-six percent ( 54 63) of the CDP showed good capacity to respond to at least one of the fractions assayed while only 18% ( 4 22) of the controls reacted. The Mc fraction showed the best specificity as well as the maximal stimulating effect in CDP ( 37 55 vs 0 18 in controls) while WH (62% vs 10%), F (54% vs 16%) and CS (52% vs 6%) showed a lesser antigenic capacity. These results suggest that recognition mechanisms and proliferative responses are functional in CDP. No correlation was found between the stimulating capacity of fractions in vitro and their immunogenic capacity previously observed in vivo. © 1983.
format JOUR
author De Titto, E.H.
Segura, E.L.
Braun, M.
author_facet De Titto, E.H.
Segura, E.L.
Braun, M.
author_sort De Titto, E.H.
title Cellular immunity in chagas' disease patients. Lymphoproliferative response to subcellular fractions of Trypanosoma cruzi
title_short Cellular immunity in chagas' disease patients. Lymphoproliferative response to subcellular fractions of Trypanosoma cruzi
title_full Cellular immunity in chagas' disease patients. Lymphoproliferative response to subcellular fractions of Trypanosoma cruzi
title_fullStr Cellular immunity in chagas' disease patients. Lymphoproliferative response to subcellular fractions of Trypanosoma cruzi
title_full_unstemmed Cellular immunity in chagas' disease patients. Lymphoproliferative response to subcellular fractions of Trypanosoma cruzi
title_sort cellular immunity in chagas' disease patients. lymphoproliferative response to subcellular fractions of trypanosoma cruzi
url http://hdl.handle.net/20.500.12110/paper_01652478_v6_n3_p161_DeTitto
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AT segurael cellularimmunityinchagasdiseasepatientslymphoproliferativeresponsetosubcellularfractionsoftrypanosomacruzi
AT braunm cellularimmunityinchagasdiseasepatientslymphoproliferativeresponsetosubcellularfractionsoftrypanosomacruzi
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