Identity of the major cysteine proteinase (cruzipain) from Trypanosoma cruzi and an antigen (Ag163B6) isolated with a monoclonal antibody

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Cruzipain, purified by conventional methods, and Ag163B6, isolated by affinity chromatography with a monoclonal antibody raised against a T. cruzi extract, are glycoproteins with a similar electrophoretic mobility, which reacted with sera from most chronic chagasic patients. Their behaviour in SDS-P...

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Autores principales: Malchiodi, E.L., Chiaramonte, M.G., Martinez, J.A., Zwirner, N.W., Margni, R.A., Cazzulo, J.J.
Formato: JOUR
Materias:
Ag163B6
Cruzipain
monoclonal antibody
parasite antigen
affinity chromatography
article
mouse
nonhuman
priority journal
trypanosoma cruzi
Animal
Antibodies, Monoclonal
Antigens, Protozoan
Blotting, Western
Cysteine Endopeptidases
Electrophoresis, Gel, Two-Dimensional
Immunodiffusion
Isoelectric Focusing
Support, Non-U.S. Gov't
Trypanosoma cruzi
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_01652478_v35_n1_p59_Malchiodi
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_01652478_v35_n1_p59_Malchiodi
record_format dspace
spelling todo:paper_01652478_v35_n1_p59_Malchiodi2023-10-03T15:02:53Z Identity of the major cysteine proteinase (cruzipain) from Trypanosoma cruzi and an antigen (Ag163B6) isolated with a monoclonal antibody Malchiodi, E.L. Chiaramonte, M.G. Martinez, J.A. Zwirner, N.W. Margni, R.A. Cazzulo, J.J. Ag163B6 Cruzipain monoclonal antibody parasite antigen affinity chromatography article mouse nonhuman priority journal trypanosoma cruzi Animal Antibodies, Monoclonal Antigens, Protozoan Blotting, Western Cysteine Endopeptidases Electrophoresis, Gel, Two-Dimensional Immunodiffusion Isoelectric Focusing Support, Non-U.S. Gov't Trypanosoma cruzi Cruzipain, purified by conventional methods, and Ag163B6, isolated by affinity chromatography with a monoclonal antibody raised against a T. cruzi extract, are glycoproteins with a similar electrophoretic mobility, which reacted with sera from most chronic chagasic patients. Their behaviour in SDS-PAGE, Western blotting, isoelectric focusing, two-dimensional electrophoresis (IEF and SDS-PAGE), Ouchterlony's double diffusion, and enzyme activity in SDS-PAGE gels containing 0.1% gelatin suggests that they are identical. © 1993. Fil:Martinez, J.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01652478_v35_n1_p59_Malchiodi
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Ag163B6
Cruzipain
monoclonal antibody
parasite antigen
affinity chromatography
article
mouse
nonhuman
priority journal
trypanosoma cruzi
Animal
Antibodies, Monoclonal
Antigens, Protozoan
Blotting, Western
Cysteine Endopeptidases
Electrophoresis, Gel, Two-Dimensional
Immunodiffusion
Isoelectric Focusing
Support, Non-U.S. Gov't
Trypanosoma cruzi
spellingShingle Ag163B6
Cruzipain
monoclonal antibody
parasite antigen
affinity chromatography
article
mouse
nonhuman
priority journal
trypanosoma cruzi
Animal
Antibodies, Monoclonal
Antigens, Protozoan
Blotting, Western
Cysteine Endopeptidases
Electrophoresis, Gel, Two-Dimensional
Immunodiffusion
Isoelectric Focusing
Support, Non-U.S. Gov't
Trypanosoma cruzi
Malchiodi, E.L.
Chiaramonte, M.G.
Martinez, J.A.
Zwirner, N.W.
Margni, R.A.
Cazzulo, J.J.
Identity of the major cysteine proteinase (cruzipain) from Trypanosoma cruzi and an antigen (Ag163B6) isolated with a monoclonal antibody
topic_facet Ag163B6
Cruzipain
monoclonal antibody
parasite antigen
affinity chromatography
article
mouse
nonhuman
priority journal
trypanosoma cruzi
Animal
Antibodies, Monoclonal
Antigens, Protozoan
Blotting, Western
Cysteine Endopeptidases
Electrophoresis, Gel, Two-Dimensional
Immunodiffusion
Isoelectric Focusing
Support, Non-U.S. Gov't
Trypanosoma cruzi
description Cruzipain, purified by conventional methods, and Ag163B6, isolated by affinity chromatography with a monoclonal antibody raised against a T. cruzi extract, are glycoproteins with a similar electrophoretic mobility, which reacted with sera from most chronic chagasic patients. Their behaviour in SDS-PAGE, Western blotting, isoelectric focusing, two-dimensional electrophoresis (IEF and SDS-PAGE), Ouchterlony's double diffusion, and enzyme activity in SDS-PAGE gels containing 0.1% gelatin suggests that they are identical. © 1993.
format JOUR
author Malchiodi, E.L.
Chiaramonte, M.G.
Martinez, J.A.
Zwirner, N.W.
Margni, R.A.
Cazzulo, J.J.
author_facet Malchiodi, E.L.
Chiaramonte, M.G.
Martinez, J.A.
Zwirner, N.W.
Margni, R.A.
Cazzulo, J.J.
author_sort Malchiodi, E.L.
title Identity of the major cysteine proteinase (cruzipain) from Trypanosoma cruzi and an antigen (Ag163B6) isolated with a monoclonal antibody
title_short Identity of the major cysteine proteinase (cruzipain) from Trypanosoma cruzi and an antigen (Ag163B6) isolated with a monoclonal antibody
title_full Identity of the major cysteine proteinase (cruzipain) from Trypanosoma cruzi and an antigen (Ag163B6) isolated with a monoclonal antibody
title_fullStr Identity of the major cysteine proteinase (cruzipain) from Trypanosoma cruzi and an antigen (Ag163B6) isolated with a monoclonal antibody
title_full_unstemmed Identity of the major cysteine proteinase (cruzipain) from Trypanosoma cruzi and an antigen (Ag163B6) isolated with a monoclonal antibody
title_sort identity of the major cysteine proteinase (cruzipain) from trypanosoma cruzi and an antigen (ag163b6) isolated with a monoclonal antibody
url http://hdl.handle.net/20.500.12110/paper_01652478_v35_n1_p59_Malchiodi
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AT cazzulojj identityofthemajorcysteineproteinasecruzipainfromtrypanosomacruziandanantigenag163b6isolatedwithamonoclonalantibody
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