Potential contraceptive use of epididymal proteins: evidence for the participation of specific antibodies against rat epididymal protein DE in male and female fertility inhibition
Previous studies in our laboratory indicated that immunization of male and female Wistar and Lewis rats with epididymal protein DE, resulted in the development of anti-DE antibodies in over 90% of the animals, with a significant and reversible reduction of fertility. In the present study, ELISA assa...
Guardado en:
| Autores principales: | , , |
|---|---|
| Formato: | JOUR |
| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_01650378_v29_n1_p31_Martinez |
| Aporte de: |
| id |
todo:paper_01650378_v29_n1_p31_Martinez |
|---|---|
| record_format |
dspace |
| spelling |
todo:paper_01650378_v29_n1_p31_Martinez2023-10-03T15:02:38Z Potential contraceptive use of epididymal proteins: evidence for the participation of specific antibodies against rat epididymal protein DE in male and female fertility inhibition Martinez, S.P. Conesa, D. Cuasnicú, P.S. Contraception Epididymal proteins Immunization Spermatozoa antibody animal cell animal experiment animal tissue article artificial insemination contraception enzyme linked immunosorbent assay epididymis evolution female female fertility immune response immunization immunoblotting immunofluorescence male nonhuman priority journal rat spermatozoon spermatozoon capacitation spermatozoon motility Animal Blotting, Western Contraception, Immunologic Epididymal Secretory Proteins Epididymis Female Immune Sera Immunization Isoantibodies Male Metalloproteins Rats Rats, Inbred Lew Rats, Wistar Sperm Agglutination Sperm Capacitation Sperm Motility Sperm-Ovum Interactions Support, Non-U.S. Gov't Testicular Hormones Previous studies in our laboratory indicated that immunization of male and female Wistar and Lewis rats with epididymal protein DE, resulted in the development of anti-DE antibodies in over 90% of the animals, with a significant and reversible reduction of fertility. In the present study, ELISA assays performed to analyze the evolution of the immune response indicated that antibody levels in the sera of immunized animals reached a maximum at 8 weeks after the initial injection and then gradually decreased, returning to control values by the end of the sixth month. Western blot experiments demonstrated that the immune sera specifically recognized DE in epididymal sperm extracts and epididymal cytosol, while no reaction was observed with different reproductive and essential organs. The immune sera were also capable of recognizing DE on the surface of both fresh and capacitated sperm as indicated by indirect immunofluorescence experiments. Finally, the exposure of sperm to immune sera prior to uterine insemination resulted in a significant (P < 0.05) reduction in the percentage of fertilized eggs compared to controls, with no effect on sperm motility and viability, nor on their ability to undergo capacitation. Together, these results support the participation of the raised antibodies as mediators of the antifertility effect and suggest a specific interference at the sperm-egg interaction level. © 1995. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01650378_v29_n1_p31_Martinez |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Contraception Epididymal proteins Immunization Spermatozoa antibody animal cell animal experiment animal tissue article artificial insemination contraception enzyme linked immunosorbent assay epididymis evolution female female fertility immune response immunization immunoblotting immunofluorescence male nonhuman priority journal rat spermatozoon spermatozoon capacitation spermatozoon motility Animal Blotting, Western Contraception, Immunologic Epididymal Secretory Proteins Epididymis Female Immune Sera Immunization Isoantibodies Male Metalloproteins Rats Rats, Inbred Lew Rats, Wistar Sperm Agglutination Sperm Capacitation Sperm Motility Sperm-Ovum Interactions Support, Non-U.S. Gov't Testicular Hormones |
| spellingShingle |
Contraception Epididymal proteins Immunization Spermatozoa antibody animal cell animal experiment animal tissue article artificial insemination contraception enzyme linked immunosorbent assay epididymis evolution female female fertility immune response immunization immunoblotting immunofluorescence male nonhuman priority journal rat spermatozoon spermatozoon capacitation spermatozoon motility Animal Blotting, Western Contraception, Immunologic Epididymal Secretory Proteins Epididymis Female Immune Sera Immunization Isoantibodies Male Metalloproteins Rats Rats, Inbred Lew Rats, Wistar Sperm Agglutination Sperm Capacitation Sperm Motility Sperm-Ovum Interactions Support, Non-U.S. Gov't Testicular Hormones Martinez, S.P. Conesa, D. Cuasnicú, P.S. Potential contraceptive use of epididymal proteins: evidence for the participation of specific antibodies against rat epididymal protein DE in male and female fertility inhibition |
| topic_facet |
Contraception Epididymal proteins Immunization Spermatozoa antibody animal cell animal experiment animal tissue article artificial insemination contraception enzyme linked immunosorbent assay epididymis evolution female female fertility immune response immunization immunoblotting immunofluorescence male nonhuman priority journal rat spermatozoon spermatozoon capacitation spermatozoon motility Animal Blotting, Western Contraception, Immunologic Epididymal Secretory Proteins Epididymis Female Immune Sera Immunization Isoantibodies Male Metalloproteins Rats Rats, Inbred Lew Rats, Wistar Sperm Agglutination Sperm Capacitation Sperm Motility Sperm-Ovum Interactions Support, Non-U.S. Gov't Testicular Hormones |
| description |
Previous studies in our laboratory indicated that immunization of male and female Wistar and Lewis rats with epididymal protein DE, resulted in the development of anti-DE antibodies in over 90% of the animals, with a significant and reversible reduction of fertility. In the present study, ELISA assays performed to analyze the evolution of the immune response indicated that antibody levels in the sera of immunized animals reached a maximum at 8 weeks after the initial injection and then gradually decreased, returning to control values by the end of the sixth month. Western blot experiments demonstrated that the immune sera specifically recognized DE in epididymal sperm extracts and epididymal cytosol, while no reaction was observed with different reproductive and essential organs. The immune sera were also capable of recognizing DE on the surface of both fresh and capacitated sperm as indicated by indirect immunofluorescence experiments. Finally, the exposure of sperm to immune sera prior to uterine insemination resulted in a significant (P < 0.05) reduction in the percentage of fertilized eggs compared to controls, with no effect on sperm motility and viability, nor on their ability to undergo capacitation. Together, these results support the participation of the raised antibodies as mediators of the antifertility effect and suggest a specific interference at the sperm-egg interaction level. © 1995. |
| format |
JOUR |
| author |
Martinez, S.P. Conesa, D. Cuasnicú, P.S. |
| author_facet |
Martinez, S.P. Conesa, D. Cuasnicú, P.S. |
| author_sort |
Martinez, S.P. |
| title |
Potential contraceptive use of epididymal proteins: evidence for the participation of specific antibodies against rat epididymal protein DE in male and female fertility inhibition |
| title_short |
Potential contraceptive use of epididymal proteins: evidence for the participation of specific antibodies against rat epididymal protein DE in male and female fertility inhibition |
| title_full |
Potential contraceptive use of epididymal proteins: evidence for the participation of specific antibodies against rat epididymal protein DE in male and female fertility inhibition |
| title_fullStr |
Potential contraceptive use of epididymal proteins: evidence for the participation of specific antibodies against rat epididymal protein DE in male and female fertility inhibition |
| title_full_unstemmed |
Potential contraceptive use of epididymal proteins: evidence for the participation of specific antibodies against rat epididymal protein DE in male and female fertility inhibition |
| title_sort |
potential contraceptive use of epididymal proteins: evidence for the participation of specific antibodies against rat epididymal protein de in male and female fertility inhibition |
| url |
http://hdl.handle.net/20.500.12110/paper_01650378_v29_n1_p31_Martinez |
| work_keys_str_mv |
AT martinezsp potentialcontraceptiveuseofepididymalproteinsevidencefortheparticipationofspecificantibodiesagainstratepididymalproteindeinmaleandfemalefertilityinhibition AT conesad potentialcontraceptiveuseofepididymalproteinsevidencefortheparticipationofspecificantibodiesagainstratepididymalproteindeinmaleandfemalefertilityinhibition AT cuasnicups potentialcontraceptiveuseofepididymalproteinsevidencefortheparticipationofspecificantibodiesagainstratepididymalproteindeinmaleandfemalefertilityinhibition |
| _version_ |
1807317129498722304 |