Alloimmunization-induced changes in β-adrenoceptor expression and cAMP on B lymphocytes

In this report we have examined the effect of alloimmunization on β-adrenergic expression in lymphocytes. We have observed a variation in the number of β-adrenoceptors (Bmax) according to the degree of immunization without modifications in their affinity (Kd). This phenomenon was accompanied with pa...

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Autores principales:	Genaro, A.M., Borda, E.
Formato:	JOUR
Materias:	Alloimmunization B cell cAMP Lymphocyte β-Adrenoceptor alloantigen beta adrenergic receptor cyclic amp dihydroalprenolol b lymphocyte cell culture immunization mouse nonhuman priority journal Animal B-Lymphocytes Cyclic AMP Cytotoxicity Tests, Immunologic Dihydroalprenolol Immunization Isoantigens Mice Mice, Inbred BALB C Mice, Inbred C3H Receptors, Adrenergic, beta Spleen
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_01623109_v18_n1_p63_Genaro
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_01623109_v18_n1_p63_Genaro
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spelling	todo:paper_01623109_v18_n1_p63_Genaro2023-10-03T15:01:36Z Alloimmunization-induced changes in β-adrenoceptor expression and cAMP on B lymphocytes Genaro, A.M. Borda, E. Alloimmunization B cell cAMP Lymphocyte β-Adrenoceptor alloantigen beta adrenergic receptor cyclic amp dihydroalprenolol b lymphocyte cell culture immunization mouse nonhuman priority journal Animal B-Lymphocytes Cyclic AMP Cytotoxicity Tests, Immunologic Dihydroalprenolol Immunization Isoantigens Mice Mice, Inbred BALB C Mice, Inbred C3H Receptors, Adrenergic, beta Spleen In this report we have examined the effect of alloimmunization on β-adrenergic expression in lymphocytes. We have observed a variation in the number of β-adrenoceptors (Bmax) according to the degree of immunization without modifications in their affinity (Kd). This phenomenon was accompanied with parallel variations of intracellular cAMP levels. A decrease in Bmax values was observed during the first and second immunizations. Then the Bmax began to increase, exceeding control values up to the fourth and fifth immunizations, and remaining constant at the sixth immunizations. Only B cell-enriched populations showed variation in Bmax values of β-adrenoceptors with alloimmunization. In contrast, the Bmax values of T cell-enriched populations did not change. Kd values were similar in all cell types tested. The number of binding sites was not dependent on the animal's age. Modifications in cAMP levels of B cell-enriched populations were correlated with changes in β-adrenoceptor expression. These results suggest that β-adrenoceptor expression and cAMP intracellular levels in B cell-enriched populations vary with the number of alloimmunizations. In addition, the antibody synthesis induced by allogenic stimulus was inversely proportional to the number of β-adrenoceptors expressed on B cells. Perhaps these findings are evidence of a control mechanism that regulates antibody synthesis during the immune response. © 1989. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01623109_v18_n1_p63_Genaro
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Alloimmunization B cell cAMP Lymphocyte β-Adrenoceptor alloantigen beta adrenergic receptor cyclic amp dihydroalprenolol b lymphocyte cell culture immunization mouse nonhuman priority journal Animal B-Lymphocytes Cyclic AMP Cytotoxicity Tests, Immunologic Dihydroalprenolol Immunization Isoantigens Mice Mice, Inbred BALB C Mice, Inbred C3H Receptors, Adrenergic, beta Spleen
spellingShingle	Alloimmunization B cell cAMP Lymphocyte β-Adrenoceptor alloantigen beta adrenergic receptor cyclic amp dihydroalprenolol b lymphocyte cell culture immunization mouse nonhuman priority journal Animal B-Lymphocytes Cyclic AMP Cytotoxicity Tests, Immunologic Dihydroalprenolol Immunization Isoantigens Mice Mice, Inbred BALB C Mice, Inbred C3H Receptors, Adrenergic, beta Spleen Genaro, A.M. Borda, E. Alloimmunization-induced changes in β-adrenoceptor expression and cAMP on B lymphocytes
topic_facet	Alloimmunization B cell cAMP Lymphocyte β-Adrenoceptor alloantigen beta adrenergic receptor cyclic amp dihydroalprenolol b lymphocyte cell culture immunization mouse nonhuman priority journal Animal B-Lymphocytes Cyclic AMP Cytotoxicity Tests, Immunologic Dihydroalprenolol Immunization Isoantigens Mice Mice, Inbred BALB C Mice, Inbred C3H Receptors, Adrenergic, beta Spleen
description	In this report we have examined the effect of alloimmunization on β-adrenergic expression in lymphocytes. We have observed a variation in the number of β-adrenoceptors (Bmax) according to the degree of immunization without modifications in their affinity (Kd). This phenomenon was accompanied with parallel variations of intracellular cAMP levels. A decrease in Bmax values was observed during the first and second immunizations. Then the Bmax began to increase, exceeding control values up to the fourth and fifth immunizations, and remaining constant at the sixth immunizations. Only B cell-enriched populations showed variation in Bmax values of β-adrenoceptors with alloimmunization. In contrast, the Bmax values of T cell-enriched populations did not change. Kd values were similar in all cell types tested. The number of binding sites was not dependent on the animal's age. Modifications in cAMP levels of B cell-enriched populations were correlated with changes in β-adrenoceptor expression. These results suggest that β-adrenoceptor expression and cAMP intracellular levels in B cell-enriched populations vary with the number of alloimmunizations. In addition, the antibody synthesis induced by allogenic stimulus was inversely proportional to the number of β-adrenoceptors expressed on B cells. Perhaps these findings are evidence of a control mechanism that regulates antibody synthesis during the immune response. © 1989.
format	JOUR
author	Genaro, A.M. Borda, E.
author_facet	Genaro, A.M. Borda, E.
author_sort	Genaro, A.M.
title	Alloimmunization-induced changes in β-adrenoceptor expression and cAMP on B lymphocytes
title_short	Alloimmunization-induced changes in β-adrenoceptor expression and cAMP on B lymphocytes
title_full	Alloimmunization-induced changes in β-adrenoceptor expression and cAMP on B lymphocytes
title_fullStr	Alloimmunization-induced changes in β-adrenoceptor expression and cAMP on B lymphocytes
title_full_unstemmed	Alloimmunization-induced changes in β-adrenoceptor expression and cAMP on B lymphocytes
title_sort	alloimmunization-induced changes in β-adrenoceptor expression and camp on b lymphocytes
url	http://hdl.handle.net/20.500.12110/paper_01623109_v18_n1_p63_Genaro
work_keys_str_mv	AT genaroam alloimmunizationinducedchangesinbadrenoceptorexpressionandcamponblymphocytes AT bordae alloimmunizationinducedchangesinbadrenoceptorexpressionandcamponblymphocytes
_version_	1807315010137882624

Alloimmunization-induced changes in β-adrenoceptor expression and cAMP on B lymphocytes

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