The [Ru(Hedta)NO] 0,1- system: Structure, chemical reactivity and biological assays
The [Ru II(Hedta)NO +] complex is a diamagnetic species crystallizing in a distorted octahedral geometry, with the Ru-N(O) length 1.756(4) Å and the RuNO angle 172.3(4)°. The complex contains one protonated carboxylate (pK a = 2.7 ± 0.1). The [Ru II(Hedta)NO +] complex undergoes a nitrosyl-centered...
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todo:paper_01620134_v98_n11_p1921_Zanichelli2023-10-03T15:01:30Z The [Ru(Hedta)NO] 0,1- system: Structure, chemical reactivity and biological assays Zanichelli, P.G. Miotto, A.M. Estrela, H.F.G. Soares, F.R. Grassi-Kassisse, D.M. Spadari-Bratfisch, R.C. Castellano, E.E. Roncaroli, F. Parise, A.R. Olabe, J.A. De Brito, A.R.M.S. Franco, D.W. Nitrosyl reactivity NO-donors Nucleophilic reactivity Ruthenium nitrosyl Vasodilator activity carboxylic acid hydroxide metal nitric oxide nitroprusside sodium ruthenium derivative addition reaction animal experiment article bioaccumulation bioassay chemical reaction controlled study crystallization death drug effect drug structure electricity electrochemistry electron endothelium geometry injection kidney liver male nonhuman parameter plasma proton transport rat reaction analysis reduction sample structure analysis time toxicity urine vascular ring vasodilatation Animals Crystallography, X-Ray Edetic Acid Electrochemistry Male Mice Models, Molecular Molecular Conformation Nitric Oxide Ruthenium Tissue Distribution Xenox The [Ru II(Hedta)NO +] complex is a diamagnetic species crystallizing in a distorted octahedral geometry, with the Ru-N(O) length 1.756(4) Å and the RuNO angle 172.3(4)°. The complex contains one protonated carboxylate (pK a = 2.7 ± 0.1). The [Ru II(Hedta)NO +] complex undergoes a nitrosyl-centered one-electron reduction (chemical or electrochemical), with E NO+/NO = -0.31 V vs SCE (I = 0.2 M, pH 1), yielding [Ru II(Hedta)NO] -, which aquates slowly: k -NO = 2.1 ± 0.4 × 10 -3 s -1 (pH 1.0, I = 0.2 M, CF 3COOH/NaCF 3COO, 25°C). At pHs > 12, the predominant species, [Ru II(edta)NO] -, reacts according to [Ru II(edta) NO] - + 2OH - ⇒ [Ru II(edta)NO 2] 3-, with K eq = 1.0 ± 0.4 × 10 3 M -2 (I = 1.0 M, NaCl; T = 25.0 ± 0.1°C). The rate-law is first order in each of the reactants for most reaction conditions, with k OH - = 4.35 ± 0.02 M -1 s -1 (25.0°C), assignable mechanistically to the elementary step comprising the attack of one OH - on [Ru II(edta)NO] -, with subsequent fast deprotonation of the [Ru II(edta) NO 2H] 2- intermediate. The activation parameters were ΔH # = 60 ± 1 kJ/mol, ΔS # = -31 ± 3 J/K mol, consistent with a nucleophilic addition process between likely charged ions. In the toxicity up-and-down tests performed with Swiss mice, no death was observed in all the doses administered (3-9.08 × 10 -5 mol/kg). The biodistribution tests performed with Wistar male rats showed metal in the liver, kidney, urine and plasma. Eight hours after the injection no metal was detected in the samples. The vasodilator effect of [Ru II(edta)NO] - was studied in aortic rings without endothelium, and was compared with sodium nitroprusside (SNP). The times of maximal effects of [Ru II(edta)NO] - and SNP were 2 h and 12 min, respectively, suggesting that [Ru II(edta)NO] - releases NO slowly to the medium in comparison with SNP. © 2004 Elsevier Inc. All rights reserved. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01620134_v98_n11_p1921_Zanichelli |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Nitrosyl reactivity NO-donors Nucleophilic reactivity Ruthenium nitrosyl Vasodilator activity carboxylic acid hydroxide metal nitric oxide nitroprusside sodium ruthenium derivative addition reaction animal experiment article bioaccumulation bioassay chemical reaction controlled study crystallization death drug effect drug structure electricity electrochemistry electron endothelium geometry injection kidney liver male nonhuman parameter plasma proton transport rat reaction analysis reduction sample structure analysis time toxicity urine vascular ring vasodilatation Animals Crystallography, X-Ray Edetic Acid Electrochemistry Male Mice Models, Molecular Molecular Conformation Nitric Oxide Ruthenium Tissue Distribution Xenox |
| spellingShingle |
Nitrosyl reactivity NO-donors Nucleophilic reactivity Ruthenium nitrosyl Vasodilator activity carboxylic acid hydroxide metal nitric oxide nitroprusside sodium ruthenium derivative addition reaction animal experiment article bioaccumulation bioassay chemical reaction controlled study crystallization death drug effect drug structure electricity electrochemistry electron endothelium geometry injection kidney liver male nonhuman parameter plasma proton transport rat reaction analysis reduction sample structure analysis time toxicity urine vascular ring vasodilatation Animals Crystallography, X-Ray Edetic Acid Electrochemistry Male Mice Models, Molecular Molecular Conformation Nitric Oxide Ruthenium Tissue Distribution Xenox Zanichelli, P.G. Miotto, A.M. Estrela, H.F.G. Soares, F.R. Grassi-Kassisse, D.M. Spadari-Bratfisch, R.C. Castellano, E.E. Roncaroli, F. Parise, A.R. Olabe, J.A. De Brito, A.R.M.S. Franco, D.W. The [Ru(Hedta)NO] 0,1- system: Structure, chemical reactivity and biological assays |
| topic_facet |
Nitrosyl reactivity NO-donors Nucleophilic reactivity Ruthenium nitrosyl Vasodilator activity carboxylic acid hydroxide metal nitric oxide nitroprusside sodium ruthenium derivative addition reaction animal experiment article bioaccumulation bioassay chemical reaction controlled study crystallization death drug effect drug structure electricity electrochemistry electron endothelium geometry injection kidney liver male nonhuman parameter plasma proton transport rat reaction analysis reduction sample structure analysis time toxicity urine vascular ring vasodilatation Animals Crystallography, X-Ray Edetic Acid Electrochemistry Male Mice Models, Molecular Molecular Conformation Nitric Oxide Ruthenium Tissue Distribution Xenox |
| description |
The [Ru II(Hedta)NO +] complex is a diamagnetic species crystallizing in a distorted octahedral geometry, with the Ru-N(O) length 1.756(4) Å and the RuNO angle 172.3(4)°. The complex contains one protonated carboxylate (pK a = 2.7 ± 0.1). The [Ru II(Hedta)NO +] complex undergoes a nitrosyl-centered one-electron reduction (chemical or electrochemical), with E NO+/NO = -0.31 V vs SCE (I = 0.2 M, pH 1), yielding [Ru II(Hedta)NO] -, which aquates slowly: k -NO = 2.1 ± 0.4 × 10 -3 s -1 (pH 1.0, I = 0.2 M, CF 3COOH/NaCF 3COO, 25°C). At pHs > 12, the predominant species, [Ru II(edta)NO] -, reacts according to [Ru II(edta) NO] - + 2OH - ⇒ [Ru II(edta)NO 2] 3-, with K eq = 1.0 ± 0.4 × 10 3 M -2 (I = 1.0 M, NaCl; T = 25.0 ± 0.1°C). The rate-law is first order in each of the reactants for most reaction conditions, with k OH - = 4.35 ± 0.02 M -1 s -1 (25.0°C), assignable mechanistically to the elementary step comprising the attack of one OH - on [Ru II(edta)NO] -, with subsequent fast deprotonation of the [Ru II(edta) NO 2H] 2- intermediate. The activation parameters were ΔH # = 60 ± 1 kJ/mol, ΔS # = -31 ± 3 J/K mol, consistent with a nucleophilic addition process between likely charged ions. In the toxicity up-and-down tests performed with Swiss mice, no death was observed in all the doses administered (3-9.08 × 10 -5 mol/kg). The biodistribution tests performed with Wistar male rats showed metal in the liver, kidney, urine and plasma. Eight hours after the injection no metal was detected in the samples. The vasodilator effect of [Ru II(edta)NO] - was studied in aortic rings without endothelium, and was compared with sodium nitroprusside (SNP). The times of maximal effects of [Ru II(edta)NO] - and SNP were 2 h and 12 min, respectively, suggesting that [Ru II(edta)NO] - releases NO slowly to the medium in comparison with SNP. © 2004 Elsevier Inc. All rights reserved. |
| format |
JOUR |
| author |
Zanichelli, P.G. Miotto, A.M. Estrela, H.F.G. Soares, F.R. Grassi-Kassisse, D.M. Spadari-Bratfisch, R.C. Castellano, E.E. Roncaroli, F. Parise, A.R. Olabe, J.A. De Brito, A.R.M.S. Franco, D.W. |
| author_facet |
Zanichelli, P.G. Miotto, A.M. Estrela, H.F.G. Soares, F.R. Grassi-Kassisse, D.M. Spadari-Bratfisch, R.C. Castellano, E.E. Roncaroli, F. Parise, A.R. Olabe, J.A. De Brito, A.R.M.S. Franco, D.W. |
| author_sort |
Zanichelli, P.G. |
| title |
The [Ru(Hedta)NO] 0,1- system: Structure, chemical reactivity and biological assays |
| title_short |
The [Ru(Hedta)NO] 0,1- system: Structure, chemical reactivity and biological assays |
| title_full |
The [Ru(Hedta)NO] 0,1- system: Structure, chemical reactivity and biological assays |
| title_fullStr |
The [Ru(Hedta)NO] 0,1- system: Structure, chemical reactivity and biological assays |
| title_full_unstemmed |
The [Ru(Hedta)NO] 0,1- system: Structure, chemical reactivity and biological assays |
| title_sort |
[ru(hedta)no] 0,1- system: structure, chemical reactivity and biological assays |
| url |
http://hdl.handle.net/20.500.12110/paper_01620134_v98_n11_p1921_Zanichelli |
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