Disturbance of cellular iron uptake and utilisation by aluminium

Aluminium (Al) affects erythropoiesis but the real mechanism of action is still unknown. Transferrin receptors (TfR) in K562 cells are able to bind Tf, when carrying either iron (Fe) or Al, with similar affinity. Then, the aim of this work was to determine whether Al could interfere with the cellula...

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Autores principales:	Pérez, G., Garbossa, G., Di Risio, C., Vittori, D., Nesse, A.
Formato:	JOUR
Materias:	Aluminium toxicity Haem synthesis Haemoglobin synthesis Iron metabolism K562 cell line aluminum butyric acid heme hemin iron 59 transferrin receptor binding affinity cell count cell culture cell differentiation cell growth cell level cell metabolism cell stimulation cell strain conference paper controlled study erythroid cell erythropoiesis heme synthesis hemoglobin synthesis human human cell iron transport receptor binding Aluminum Apoproteins Barbiturates Biological Transport Cell Differentiation Heme Hemin Hemoglobins Humans Iron K562 Cells Leukemia, Erythroblastic, Acute Radioisotopes Transferrin
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_01620134_v87_n1_p21_Perez
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_01620134_v87_n1_p21_Perez
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spelling	todo:paper_01620134_v87_n1_p21_Perez2023-10-03T15:01:30Z Disturbance of cellular iron uptake and utilisation by aluminium Pérez, G. Garbossa, G. Di Risio, C. Vittori, D. Nesse, A. Aluminium toxicity Haem synthesis Haemoglobin synthesis Iron metabolism K562 cell line aluminum butyric acid heme hemin iron 59 transferrin receptor binding affinity cell count cell culture cell differentiation cell growth cell level cell metabolism cell stimulation cell strain conference paper controlled study erythroid cell erythropoiesis heme synthesis hemoglobin synthesis human human cell iron transport receptor binding Aluminum Apoproteins Barbiturates Biological Transport Cell Differentiation Heme Hemin Hemoglobins Humans Iron K562 Cells Leukemia, Erythroblastic, Acute Radioisotopes Transferrin Aluminium (Al) affects erythropoiesis but the real mechanism of action is still unknown. Transferrin receptors (TfR) in K562 cells are able to bind Tf, when carrying either iron (Fe) or Al, with similar affinity. Then, the aim of this work was to determine whether Al could interfere with the cellular Fe uptake and utilisation. K562 cells were induced to erythroid differentiation by either haemin (H) or sodium butyrate (B) and cultured with and without Al. The effect of Al on cellular Fe uptake, Fe incorporation to haem and cell differentiation was studied. H- and B-stimulated cells grown in the presence of 10 μM Al showed a reduction in the number of haemoglobinised cells (by 18% and 56%, respectively) and high amounts of Al content. Al2Tf inhibited both the 59Fe cellular uptake and its utilisation for haem synthesis. The removal of Al during the 59Fe pulse, after a previous incubation with the metal, allowed the cells to acquire Fe quantities in the normal range or even exceeding the amounts incorporated by the respective control cells. However, the Fe incorporated to haem could not reach control values in B-stimulated cells despite enough Fe acquisition was observed after removing Al. Present results suggest that Al might exert either reversible or irreversible effects on the haemoglobin synthesis depending on cellular conditions. © 2001 Elsevier Science B.V. All rights reserved. Fil:Pérez, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Garbossa, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Di Risio, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Vittori, D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Nesse, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01620134_v87_n1_p21_Perez
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Aluminium toxicity Haem synthesis Haemoglobin synthesis Iron metabolism K562 cell line aluminum butyric acid heme hemin iron 59 transferrin receptor binding affinity cell count cell culture cell differentiation cell growth cell level cell metabolism cell stimulation cell strain conference paper controlled study erythroid cell erythropoiesis heme synthesis hemoglobin synthesis human human cell iron transport receptor binding Aluminum Apoproteins Barbiturates Biological Transport Cell Differentiation Heme Hemin Hemoglobins Humans Iron K562 Cells Leukemia, Erythroblastic, Acute Radioisotopes Transferrin
spellingShingle	Aluminium toxicity Haem synthesis Haemoglobin synthesis Iron metabolism K562 cell line aluminum butyric acid heme hemin iron 59 transferrin receptor binding affinity cell count cell culture cell differentiation cell growth cell level cell metabolism cell stimulation cell strain conference paper controlled study erythroid cell erythropoiesis heme synthesis hemoglobin synthesis human human cell iron transport receptor binding Aluminum Apoproteins Barbiturates Biological Transport Cell Differentiation Heme Hemin Hemoglobins Humans Iron K562 Cells Leukemia, Erythroblastic, Acute Radioisotopes Transferrin Pérez, G. Garbossa, G. Di Risio, C. Vittori, D. Nesse, A. Disturbance of cellular iron uptake and utilisation by aluminium
topic_facet	Aluminium toxicity Haem synthesis Haemoglobin synthesis Iron metabolism K562 cell line aluminum butyric acid heme hemin iron 59 transferrin receptor binding affinity cell count cell culture cell differentiation cell growth cell level cell metabolism cell stimulation cell strain conference paper controlled study erythroid cell erythropoiesis heme synthesis hemoglobin synthesis human human cell iron transport receptor binding Aluminum Apoproteins Barbiturates Biological Transport Cell Differentiation Heme Hemin Hemoglobins Humans Iron K562 Cells Leukemia, Erythroblastic, Acute Radioisotopes Transferrin
description	Aluminium (Al) affects erythropoiesis but the real mechanism of action is still unknown. Transferrin receptors (TfR) in K562 cells are able to bind Tf, when carrying either iron (Fe) or Al, with similar affinity. Then, the aim of this work was to determine whether Al could interfere with the cellular Fe uptake and utilisation. K562 cells were induced to erythroid differentiation by either haemin (H) or sodium butyrate (B) and cultured with and without Al. The effect of Al on cellular Fe uptake, Fe incorporation to haem and cell differentiation was studied. H- and B-stimulated cells grown in the presence of 10 μM Al showed a reduction in the number of haemoglobinised cells (by 18% and 56%, respectively) and high amounts of Al content. Al2Tf inhibited both the 59Fe cellular uptake and its utilisation for haem synthesis. The removal of Al during the 59Fe pulse, after a previous incubation with the metal, allowed the cells to acquire Fe quantities in the normal range or even exceeding the amounts incorporated by the respective control cells. However, the Fe incorporated to haem could not reach control values in B-stimulated cells despite enough Fe acquisition was observed after removing Al. Present results suggest that Al might exert either reversible or irreversible effects on the haemoglobin synthesis depending on cellular conditions. © 2001 Elsevier Science B.V. All rights reserved.
format	JOUR
author	Pérez, G. Garbossa, G. Di Risio, C. Vittori, D. Nesse, A.
author_facet	Pérez, G. Garbossa, G. Di Risio, C. Vittori, D. Nesse, A.
author_sort	Pérez, G.
title	Disturbance of cellular iron uptake and utilisation by aluminium
title_short	Disturbance of cellular iron uptake and utilisation by aluminium
title_full	Disturbance of cellular iron uptake and utilisation by aluminium
title_fullStr	Disturbance of cellular iron uptake and utilisation by aluminium
title_full_unstemmed	Disturbance of cellular iron uptake and utilisation by aluminium
title_sort	disturbance of cellular iron uptake and utilisation by aluminium
url	http://hdl.handle.net/20.500.12110/paper_01620134_v87_n1_p21_Perez
work_keys_str_mv	AT perezg disturbanceofcellularironuptakeandutilisationbyaluminium AT garbossag disturbanceofcellularironuptakeandutilisationbyaluminium AT dirisioc disturbanceofcellularironuptakeandutilisationbyaluminium AT vittorid disturbanceofcellularironuptakeandutilisationbyaluminium AT nessea disturbanceofcellularironuptakeandutilisationbyaluminium
_version_	1807317266193186816

Disturbance of cellular iron uptake and utilisation by aluminium

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