Peri-implantational in vivo and in vitro embryo-trophoblast development after perigestational alcohol exposure in the CD-1 mouse
Long-term pregestational ethanol exposure induced altered fertilization and preimplantation embryogenesis. We evaluated preimplantational embryo-trophoblast differentiation, growth and invasiveness after perigestational ethanol 10% ingestion for 15 days preceding and up to day 4 (treated females [TF...
Guardado en:
| Autores principales: | , , |
|---|---|
| Formato: | JOUR |
| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_01480545_v37_n2_p184_PerezTito |
| Aporte de: |
| id |
todo:paper_01480545_v37_n2_p184_PerezTito |
|---|---|
| record_format |
dspace |
| spelling |
todo:paper_01480545_v37_n2_p184_PerezTito2023-10-03T15:01:00Z Peri-implantational in vivo and in vitro embryo-trophoblast development after perigestational alcohol exposure in the CD-1 mouse Pérez-Tito, L. Bevilacqua, E. Cebral, E. Alcohol Blastocyst Differentiation Growth Mouse Trophoblast alcohol animal experiment article blastocyst body weight caloric intake cell differentiation cell division cell expansion cell growth cell invasion controlled study embryo embryo development embryo growth female fluid intake food intake in vitro study in vivo study inner cell mass micronucleus mitosis index morphology mouse nidation nonhuman preimplantation embryo prenatal exposure trophoblast Alcohol Drinking Animals Blastocyst Embryo Implantation Embryo, Mammalian Embryonic Development Female Fertilization Maternal Exposure Mice Pregnancy Time Factors Trophoblasts Long-term pregestational ethanol exposure induced altered fertilization and preimplantation embryogenesis. We evaluated preimplantational embryo-trophoblast differentiation, growth and invasiveness after perigestational ethanol 10% ingestion for 15 days preceding and up to day 4 (treated females [TF]: TF-D4 group) or 5 (TF-D5) of CD-1 gestation (control females [CF] with water). In TF-D4, expanded and hatched blastocyst numbers were significantly reduced (p<0.05) versus CF-D4. Abnormal embryos and percentage of pyknotic nuclei were increased, and early blastocyst growth (nuclear number/embryo) and mitotic index was reduced (p<0.05) versus CF-D4. On day 5 of gestation, TF-D5 presented significantly reduced total embryos and advanced embryo type 3 number versus CF-D5 (p<0.05). During in vitro development, up to 72-hour culture, TF-D5 had reduced embryo type 1 (the least developed) and 3 percentages (p<0.05) versus controls, whereas embryo type 2 percentage increased (p<0.05) versus CF-D5. Embryo-trophoblast growth was studied during culture by morphometry. Embryo size ranges were classified as small, medium and large embryos. At 48-hour culture, small and medium embryos of TF had significantly increased mean area versus CF (p<0.05), whereas large embryos had reduced mean area at 24-hour culture. Perigestational alcohol exposure up to days 4-5 induced embryo differentiation retardation, abnormal blastocyst growth and alterations of embryo-trophoblast growth and expansion during implantation, suggesting impaired regulation of trophoblast invasion and a relation with early pregnancy loss after mouse perigestational alcohol consumption. © 2014 Informa Healthcare USA, Inc. All rights reserved: reproduction in whole or part not permitted. Fil:Pérez-Tito, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Cebral, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01480545_v37_n2_p184_PerezTito |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Alcohol Blastocyst Differentiation Growth Mouse Trophoblast alcohol animal experiment article blastocyst body weight caloric intake cell differentiation cell division cell expansion cell growth cell invasion controlled study embryo embryo development embryo growth female fluid intake food intake in vitro study in vivo study inner cell mass micronucleus mitosis index morphology mouse nidation nonhuman preimplantation embryo prenatal exposure trophoblast Alcohol Drinking Animals Blastocyst Embryo Implantation Embryo, Mammalian Embryonic Development Female Fertilization Maternal Exposure Mice Pregnancy Time Factors Trophoblasts |
| spellingShingle |
Alcohol Blastocyst Differentiation Growth Mouse Trophoblast alcohol animal experiment article blastocyst body weight caloric intake cell differentiation cell division cell expansion cell growth cell invasion controlled study embryo embryo development embryo growth female fluid intake food intake in vitro study in vivo study inner cell mass micronucleus mitosis index morphology mouse nidation nonhuman preimplantation embryo prenatal exposure trophoblast Alcohol Drinking Animals Blastocyst Embryo Implantation Embryo, Mammalian Embryonic Development Female Fertilization Maternal Exposure Mice Pregnancy Time Factors Trophoblasts Pérez-Tito, L. Bevilacqua, E. Cebral, E. Peri-implantational in vivo and in vitro embryo-trophoblast development after perigestational alcohol exposure in the CD-1 mouse |
| topic_facet |
Alcohol Blastocyst Differentiation Growth Mouse Trophoblast alcohol animal experiment article blastocyst body weight caloric intake cell differentiation cell division cell expansion cell growth cell invasion controlled study embryo embryo development embryo growth female fluid intake food intake in vitro study in vivo study inner cell mass micronucleus mitosis index morphology mouse nidation nonhuman preimplantation embryo prenatal exposure trophoblast Alcohol Drinking Animals Blastocyst Embryo Implantation Embryo, Mammalian Embryonic Development Female Fertilization Maternal Exposure Mice Pregnancy Time Factors Trophoblasts |
| description |
Long-term pregestational ethanol exposure induced altered fertilization and preimplantation embryogenesis. We evaluated preimplantational embryo-trophoblast differentiation, growth and invasiveness after perigestational ethanol 10% ingestion for 15 days preceding and up to day 4 (treated females [TF]: TF-D4 group) or 5 (TF-D5) of CD-1 gestation (control females [CF] with water). In TF-D4, expanded and hatched blastocyst numbers were significantly reduced (p<0.05) versus CF-D4. Abnormal embryos and percentage of pyknotic nuclei were increased, and early blastocyst growth (nuclear number/embryo) and mitotic index was reduced (p<0.05) versus CF-D4. On day 5 of gestation, TF-D5 presented significantly reduced total embryos and advanced embryo type 3 number versus CF-D5 (p<0.05). During in vitro development, up to 72-hour culture, TF-D5 had reduced embryo type 1 (the least developed) and 3 percentages (p<0.05) versus controls, whereas embryo type 2 percentage increased (p<0.05) versus CF-D5. Embryo-trophoblast growth was studied during culture by morphometry. Embryo size ranges were classified as small, medium and large embryos. At 48-hour culture, small and medium embryos of TF had significantly increased mean area versus CF (p<0.05), whereas large embryos had reduced mean area at 24-hour culture. Perigestational alcohol exposure up to days 4-5 induced embryo differentiation retardation, abnormal blastocyst growth and alterations of embryo-trophoblast growth and expansion during implantation, suggesting impaired regulation of trophoblast invasion and a relation with early pregnancy loss after mouse perigestational alcohol consumption. © 2014 Informa Healthcare USA, Inc. All rights reserved: reproduction in whole or part not permitted. |
| format |
JOUR |
| author |
Pérez-Tito, L. Bevilacqua, E. Cebral, E. |
| author_facet |
Pérez-Tito, L. Bevilacqua, E. Cebral, E. |
| author_sort |
Pérez-Tito, L. |
| title |
Peri-implantational in vivo and in vitro embryo-trophoblast development after perigestational alcohol exposure in the CD-1 mouse |
| title_short |
Peri-implantational in vivo and in vitro embryo-trophoblast development after perigestational alcohol exposure in the CD-1 mouse |
| title_full |
Peri-implantational in vivo and in vitro embryo-trophoblast development after perigestational alcohol exposure in the CD-1 mouse |
| title_fullStr |
Peri-implantational in vivo and in vitro embryo-trophoblast development after perigestational alcohol exposure in the CD-1 mouse |
| title_full_unstemmed |
Peri-implantational in vivo and in vitro embryo-trophoblast development after perigestational alcohol exposure in the CD-1 mouse |
| title_sort |
peri-implantational in vivo and in vitro embryo-trophoblast development after perigestational alcohol exposure in the cd-1 mouse |
| url |
http://hdl.handle.net/20.500.12110/paper_01480545_v37_n2_p184_PerezTito |
| work_keys_str_mv |
AT pereztitol periimplantationalinvivoandinvitroembryotrophoblastdevelopmentafterperigestationalalcoholexposureinthecd1mouse AT bevilacquae periimplantationalinvivoandinvitroembryotrophoblastdevelopmentafterperigestationalalcoholexposureinthecd1mouse AT cebrale periimplantationalinvivoandinvitroembryotrophoblastdevelopmentafterperigestationalalcoholexposureinthecd1mouse |
| _version_ |
1807315009890418688 |