Ultrastructural alterations in Phacus brachykentron (Euglenophyta) due to excess of organic matter in the culture medium
Morphological and ultrastructural changes induced by exposure to excess of organic matter were analyzed in Phacus brachykentron (Pochm.). The cells were isolated from sites in Matanza River, Buenos Aires, Argentina, which have a high degree of organic matter contamination coming from waste waters di...
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todo:paper_01476513_v101_n1_p36_Nannavecchia2023-10-03T15:00:35Z Ultrastructural alterations in Phacus brachykentron (Euglenophyta) due to excess of organic matter in the culture medium Nannavecchia, P. Tolivia, A. Conforti, V. Bioindicator Euglenoids Microalgae Monitoring Water quality organic matter bioassay bioindicator biomonitoring concentration (composition) enrichment microalga morphology organic matter transmission electron microscopy ultrastructure wastewater algal cell culture algal growth Argentina article cell nucleus cell ultrastructure chloroplast controlled study culture medium cytolysis Euglenida growth rate industrial waste meat industry nonhuman Phacus brachykentron transmission electron microscopy water pollution Argentina Buenos Aires [Argentina] Matanza-Riachuelo River Bioindicator Euglenoids Microalgae Monitoring Water quality Biological Assay Environmental Monitoring Euglenida Microscopy, Electron, Scanning Microscopy, Electron, Transmission Water Pollutants, Chemical Morphological and ultrastructural changes induced by exposure to excess of organic matter were analyzed in Phacus brachykentron (Pochm.). The cells were isolated from sites in Matanza River, Buenos Aires, Argentina, which have a high degree of organic matter contamination coming from waste waters discharges of the meat industry. Master strains were cultured on soil water medium and a toxicity bioassay was performed. As a result of the enriched medium, several morphological and ultrastructural cellular alterations were observed by optical, scanning, and transmission electron microscopy. Among these, we can point out changes in cell dimensions, remarkable widening of some pellicle bands, increased number and volume of paramylon grains, displacement of the nucleus from the central to the lateral position, some chloroplasts with their thylakoids disordered, and cell lysis. The response to organic enrichment was very fast, i.e. during the 48. h of the bioassay. Therefore, any significant increase of organic matter would rapidly affect wild euglenoids. Our results suggest that the alterations observed, such as the presence of large intracellular paramylon bodies or the deformation of euglenoid cells in natural samples, have the potential to be used as environmental bioindicators. © 2013 Elsevier Inc. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01476513_v101_n1_p36_Nannavecchia |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Bioindicator Euglenoids Microalgae Monitoring Water quality organic matter bioassay bioindicator biomonitoring concentration (composition) enrichment microalga morphology organic matter transmission electron microscopy ultrastructure wastewater algal cell culture algal growth Argentina article cell nucleus cell ultrastructure chloroplast controlled study culture medium cytolysis Euglenida growth rate industrial waste meat industry nonhuman Phacus brachykentron transmission electron microscopy water pollution Argentina Buenos Aires [Argentina] Matanza-Riachuelo River Bioindicator Euglenoids Microalgae Monitoring Water quality Biological Assay Environmental Monitoring Euglenida Microscopy, Electron, Scanning Microscopy, Electron, Transmission Water Pollutants, Chemical |
| spellingShingle |
Bioindicator Euglenoids Microalgae Monitoring Water quality organic matter bioassay bioindicator biomonitoring concentration (composition) enrichment microalga morphology organic matter transmission electron microscopy ultrastructure wastewater algal cell culture algal growth Argentina article cell nucleus cell ultrastructure chloroplast controlled study culture medium cytolysis Euglenida growth rate industrial waste meat industry nonhuman Phacus brachykentron transmission electron microscopy water pollution Argentina Buenos Aires [Argentina] Matanza-Riachuelo River Bioindicator Euglenoids Microalgae Monitoring Water quality Biological Assay Environmental Monitoring Euglenida Microscopy, Electron, Scanning Microscopy, Electron, Transmission Water Pollutants, Chemical Nannavecchia, P. Tolivia, A. Conforti, V. Ultrastructural alterations in Phacus brachykentron (Euglenophyta) due to excess of organic matter in the culture medium |
| topic_facet |
Bioindicator Euglenoids Microalgae Monitoring Water quality organic matter bioassay bioindicator biomonitoring concentration (composition) enrichment microalga morphology organic matter transmission electron microscopy ultrastructure wastewater algal cell culture algal growth Argentina article cell nucleus cell ultrastructure chloroplast controlled study culture medium cytolysis Euglenida growth rate industrial waste meat industry nonhuman Phacus brachykentron transmission electron microscopy water pollution Argentina Buenos Aires [Argentina] Matanza-Riachuelo River Bioindicator Euglenoids Microalgae Monitoring Water quality Biological Assay Environmental Monitoring Euglenida Microscopy, Electron, Scanning Microscopy, Electron, Transmission Water Pollutants, Chemical |
| description |
Morphological and ultrastructural changes induced by exposure to excess of organic matter were analyzed in Phacus brachykentron (Pochm.). The cells were isolated from sites in Matanza River, Buenos Aires, Argentina, which have a high degree of organic matter contamination coming from waste waters discharges of the meat industry. Master strains were cultured on soil water medium and a toxicity bioassay was performed. As a result of the enriched medium, several morphological and ultrastructural cellular alterations were observed by optical, scanning, and transmission electron microscopy. Among these, we can point out changes in cell dimensions, remarkable widening of some pellicle bands, increased number and volume of paramylon grains, displacement of the nucleus from the central to the lateral position, some chloroplasts with their thylakoids disordered, and cell lysis. The response to organic enrichment was very fast, i.e. during the 48. h of the bioassay. Therefore, any significant increase of organic matter would rapidly affect wild euglenoids. Our results suggest that the alterations observed, such as the presence of large intracellular paramylon bodies or the deformation of euglenoid cells in natural samples, have the potential to be used as environmental bioindicators. © 2013 Elsevier Inc. |
| format |
JOUR |
| author |
Nannavecchia, P. Tolivia, A. Conforti, V. |
| author_facet |
Nannavecchia, P. Tolivia, A. Conforti, V. |
| author_sort |
Nannavecchia, P. |
| title |
Ultrastructural alterations in Phacus brachykentron (Euglenophyta) due to excess of organic matter in the culture medium |
| title_short |
Ultrastructural alterations in Phacus brachykentron (Euglenophyta) due to excess of organic matter in the culture medium |
| title_full |
Ultrastructural alterations in Phacus brachykentron (Euglenophyta) due to excess of organic matter in the culture medium |
| title_fullStr |
Ultrastructural alterations in Phacus brachykentron (Euglenophyta) due to excess of organic matter in the culture medium |
| title_full_unstemmed |
Ultrastructural alterations in Phacus brachykentron (Euglenophyta) due to excess of organic matter in the culture medium |
| title_sort |
ultrastructural alterations in phacus brachykentron (euglenophyta) due to excess of organic matter in the culture medium |
| url |
http://hdl.handle.net/20.500.12110/paper_01476513_v101_n1_p36_Nannavecchia |
| work_keys_str_mv |
AT nannavecchiap ultrastructuralalterationsinphacusbrachykentroneuglenophytaduetoexcessoforganicmatterintheculturemedium AT toliviaa ultrastructuralalterationsinphacusbrachykentroneuglenophytaduetoexcessoforganicmatterintheculturemedium AT confortiv ultrastructuralalterationsinphacusbrachykentroneuglenophytaduetoexcessoforganicmatterintheculturemedium |
| _version_ |
1807318884290658304 |