Insertion sequence-like elements associated with putative polyhydroxybutyrate regulatory genes in Azotobacter sp. FA8

The genes phaR, phaP, and phaF, encoding putative regulatory proteins, were found in the poly (3-hydroxybutyrate) (PHB) gene cluster of the free nitrogen-fixing bacteria Azotobacter sp. FA8. These genes were flanked by the insertion sequence ISAzsp1, belonging to the IS3 family, and a region highly...

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Detalles Bibliográficos
Autores principales: Pettinari, M.J., Chaneton, L., Vazquez, G., Steinbüchel, A., Méndez, B.S.
Formato: JOUR
Materias:
Azotobacter
Insertion sequences
PHA
phaR, phaP, phaF
PHB
Polyhydroxyalkanoates
Polyhydroxybutyrate
poly(3 hydroxybutyric acid)
polyhydroxyalkanoic acid
regulator protein
article
bacterial gene
bacterial metabolism
gene cluster
gene insertion
gene insertion sequence
genetic recombination
nonhuman
sequence homology
insertion sequences
unidentified insertion sequence
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_0147619X_v50_n1_p36_Pettinari
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
Descripción
Sumario:The genes phaR, phaP, and phaF, encoding putative regulatory proteins, were found in the poly (3-hydroxybutyrate) (PHB) gene cluster of the free nitrogen-fixing bacteria Azotobacter sp. FA8. These genes were flanked by the insertion sequence ISAzsp1, belonging to the IS3 family, and a region highly homologous to insertion sequences of the IS630 family. These are the first site-specific recombination elements to be described in association with genes involved in the metabolism of polyhydroxyalkanoates (PHAs). A possible role for ISs in the assembly of pha genes is presented. © 2003 Elsevier Science (USA). All rights reserved.

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