The effect of light and cyclic AMP metabolism on fruiting body formation in Saccobolus platensis

The ascomycete Saccobolus platensis Gamundí & Ranalli requires light to produce apothecia. It has now been found that this light requirement can be satisfied by a 24-h pulse of white light at certain stages of the sexual cycle. The addition of exogenous N6,O2′-dibutyryl adenosine 3′,5′-cycli...

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Autores principales: Galvagno, M.A., Forchiassin, F., Cantore, M.L., Passeron, S.
Formato: JOUR
Materias:
cyclic AMP metabolism
light
Saccobolus platensis
sexual morphogenesis
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_01475975_v8_n4_p334_Galvagno
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
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spelling todo:paper_01475975_v8_n4_p334_Galvagno2023-10-03T15:00:34Z The effect of light and cyclic AMP metabolism on fruiting body formation in Saccobolus platensis Galvagno, M.A. Forchiassin, F. Cantore, M.L. Passeron, S. cyclic AMP metabolism light Saccobolus platensis sexual morphogenesis The ascomycete Saccobolus platensis Gamundí & Ranalli requires light to produce apothecia. It has now been found that this light requirement can be satisfied by a 24-h pulse of white light at certain stages of the sexual cycle. The addition of exogenous N6,O2′-dibutyryl adenosine 3′,5′-cyclic monophosphate (db-cyclic AMP) to the dark growing mycelia could replace rather efficiently the inductory effect of light; cyclic AMP, N6-monobutyryl cyclic AMP, and O2′-monobutyryl cyclic AMP were less effective, while guanosine 3′,5′-cyclic monophosphate (cyclic GMP) was a very weak inducer. An inducing effect similar to that of db-cyclic AMP was obtained by the addition of 3-isobutyl-1-methylxanthine (MIX) or theophylline to cultures developing in darkness. In the presence of theophylline, endogenous cyclic AMP levels of dark-grown mycelia were several fold higher than those of control cultures. The cyclic AMP content of mycelia growing under different light regimes was measured and no significant differences were observed. However, cultures submitted to white light showed an increase in adenylate cyclase (ATP pyrophosphate-lyase (cyclizing), EC 4.6.1.1) and a decrease in cyclic AMP phosphodiesterase (3′,5′-cyclic AMP 5′-nucleotidohydrolase, EC 3.1.4.17) specific activities compared with the activities of dark-grown mycelia. The cyclic AMP phosphodiesterase activity was strongly inhibited by theophylline and by MIX. The possible role of cyclic AMP in the induction of apothecia in this species is discussed. © 1984. Fil:Galvagno, M.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Forchiassin, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Cantore, M.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01475975_v8_n4_p334_Galvagno
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic cyclic AMP metabolism
light
Saccobolus platensis
sexual morphogenesis
spellingShingle cyclic AMP metabolism
light
Saccobolus platensis
sexual morphogenesis
Galvagno, M.A.
Forchiassin, F.
Cantore, M.L.
Passeron, S.
The effect of light and cyclic AMP metabolism on fruiting body formation in Saccobolus platensis
topic_facet cyclic AMP metabolism
light
Saccobolus platensis
sexual morphogenesis
description The ascomycete Saccobolus platensis Gamundí & Ranalli requires light to produce apothecia. It has now been found that this light requirement can be satisfied by a 24-h pulse of white light at certain stages of the sexual cycle. The addition of exogenous N6,O2′-dibutyryl adenosine 3′,5′-cyclic monophosphate (db-cyclic AMP) to the dark growing mycelia could replace rather efficiently the inductory effect of light; cyclic AMP, N6-monobutyryl cyclic AMP, and O2′-monobutyryl cyclic AMP were less effective, while guanosine 3′,5′-cyclic monophosphate (cyclic GMP) was a very weak inducer. An inducing effect similar to that of db-cyclic AMP was obtained by the addition of 3-isobutyl-1-methylxanthine (MIX) or theophylline to cultures developing in darkness. In the presence of theophylline, endogenous cyclic AMP levels of dark-grown mycelia were several fold higher than those of control cultures. The cyclic AMP content of mycelia growing under different light regimes was measured and no significant differences were observed. However, cultures submitted to white light showed an increase in adenylate cyclase (ATP pyrophosphate-lyase (cyclizing), EC 4.6.1.1) and a decrease in cyclic AMP phosphodiesterase (3′,5′-cyclic AMP 5′-nucleotidohydrolase, EC 3.1.4.17) specific activities compared with the activities of dark-grown mycelia. The cyclic AMP phosphodiesterase activity was strongly inhibited by theophylline and by MIX. The possible role of cyclic AMP in the induction of apothecia in this species is discussed. © 1984.
format JOUR
author Galvagno, M.A.
Forchiassin, F.
Cantore, M.L.
Passeron, S.
author_facet Galvagno, M.A.
Forchiassin, F.
Cantore, M.L.
Passeron, S.
author_sort Galvagno, M.A.
title The effect of light and cyclic AMP metabolism on fruiting body formation in Saccobolus platensis
title_short The effect of light and cyclic AMP metabolism on fruiting body formation in Saccobolus platensis
title_full The effect of light and cyclic AMP metabolism on fruiting body formation in Saccobolus platensis
title_fullStr The effect of light and cyclic AMP metabolism on fruiting body formation in Saccobolus platensis
title_full_unstemmed The effect of light and cyclic AMP metabolism on fruiting body formation in Saccobolus platensis
title_sort effect of light and cyclic amp metabolism on fruiting body formation in saccobolus platensis
url http://hdl.handle.net/20.500.12110/paper_01475975_v8_n4_p334_Galvagno
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