Effect of aminoguanidine, a nitric oxide synthase inhibitor, on ocular infection with herpes simplex virus in Balb/c mice

PURPOSE. To study the effect of aminoguanidine (AMG), an inhibitor of nitric oxide production, on the ocular infection of Balb/c mice with herpes simplex virus (HSV) type 1 strain F and HSV-2 strain G. METHODS. Animals were treated with different amounts of AMG (0.5, O. 1, and 0.05 mg/mouse) by topi...

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Autores principales:	Benencia, F., Courrèges, M.C., Gamba, G., Cavalieri, H., Massouh, E.J.
Formato:	JOUR
Materias:	aminoguanidine nitric oxide nitric oxide synthase inhibitor animal experiment animal model animal tissue article controlled study cornea dose response eye infection Herpes simplex virus 1 Herpes simplex virus 2 male mouse mouse strain neutrophil nonhuman priority journal virus titration Administration, Topical Animals Cornea Disease Progression DNA, Viral Dose-Response Relationship, Drug Enzyme Inhibitors Guanidines Herpesvirus 1, Human Herpesvirus 2, Human Keratitis, Herpetic Mice Mice, Inbred BALB C Nitric Oxide Synthase Ophthalmic Solutions Reverse Transcriptase Polymerase Chain Reaction Trigeminal Ganglion
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_01460404_v42_n6_p1277_Benencia
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_01460404_v42_n6_p1277_Benencia
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spelling	todo:paper_01460404_v42_n6_p1277_Benencia2023-10-03T15:00:13Z Effect of aminoguanidine, a nitric oxide synthase inhibitor, on ocular infection with herpes simplex virus in Balb/c mice Benencia, F. Courrèges, M.C. Gamba, G. Cavalieri, H. Massouh, E.J. aminoguanidine nitric oxide nitric oxide synthase inhibitor animal experiment animal model animal tissue article controlled study cornea dose response eye infection Herpes simplex virus 1 Herpes simplex virus 2 male mouse mouse strain neutrophil nonhuman priority journal virus titration Administration, Topical Animals Cornea Disease Progression DNA, Viral Dose-Response Relationship, Drug Enzyme Inhibitors Guanidines Herpesvirus 1, Human Herpesvirus 2, Human Keratitis, Herpetic Mice Mice, Inbred BALB C Nitric Oxide Synthase Ophthalmic Solutions Reverse Transcriptase Polymerase Chain Reaction Trigeminal Ganglion PURPOSE. To study the effect of aminoguanidine (AMG), an inhibitor of nitric oxide production, on the ocular infection of Balb/c mice with herpes simplex virus (HSV) type 1 strain F and HSV-2 strain G. METHODS. Animals were treated with different amounts of AMG (0.5, O. 1, and 0.05 mg/mouse) by topical application in the eye from postinfection (PI) days -2 through +5, considering 0 the day of infection. At different PI days, development of herpetic keratitis was evaluated in treated and control mice. RESULTS. Treated animals showed a dose-dependent increase in ocular disease after viral infection, compared with control animals. Viral titers in ocular washings were higher in AMG-treated mice (PI day 2, HSV-1: AMG 0.5 mg, 1.3 × 103 plaque-forming units (PFU)/ml; control, O. 22 × 102 PFU/ml, P < 0.025). At PI day 3, control corneas had only scattered inflammatory cells, whereas those from treated animals showed a conspicuous infiltrate consisting primarily of neutrophils. Viral titers were also higher in brains of treated mice. These animals died earlier and in a greater proportion than control animals (percentage of mortality, PI day 12, HSV-1: AMG 0.5 mg, 40% ± 4%; control, 18% ± 3%, P < 0.05). CONCLUSIONS. These data indicate an inhibitory effect of nitric oxide on HSV ocular infection. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01460404_v42_n6_p1277_Benencia
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	aminoguanidine nitric oxide nitric oxide synthase inhibitor animal experiment animal model animal tissue article controlled study cornea dose response eye infection Herpes simplex virus 1 Herpes simplex virus 2 male mouse mouse strain neutrophil nonhuman priority journal virus titration Administration, Topical Animals Cornea Disease Progression DNA, Viral Dose-Response Relationship, Drug Enzyme Inhibitors Guanidines Herpesvirus 1, Human Herpesvirus 2, Human Keratitis, Herpetic Mice Mice, Inbred BALB C Nitric Oxide Synthase Ophthalmic Solutions Reverse Transcriptase Polymerase Chain Reaction Trigeminal Ganglion
spellingShingle	aminoguanidine nitric oxide nitric oxide synthase inhibitor animal experiment animal model animal tissue article controlled study cornea dose response eye infection Herpes simplex virus 1 Herpes simplex virus 2 male mouse mouse strain neutrophil nonhuman priority journal virus titration Administration, Topical Animals Cornea Disease Progression DNA, Viral Dose-Response Relationship, Drug Enzyme Inhibitors Guanidines Herpesvirus 1, Human Herpesvirus 2, Human Keratitis, Herpetic Mice Mice, Inbred BALB C Nitric Oxide Synthase Ophthalmic Solutions Reverse Transcriptase Polymerase Chain Reaction Trigeminal Ganglion Benencia, F. Courrèges, M.C. Gamba, G. Cavalieri, H. Massouh, E.J. Effect of aminoguanidine, a nitric oxide synthase inhibitor, on ocular infection with herpes simplex virus in Balb/c mice
topic_facet	aminoguanidine nitric oxide nitric oxide synthase inhibitor animal experiment animal model animal tissue article controlled study cornea dose response eye infection Herpes simplex virus 1 Herpes simplex virus 2 male mouse mouse strain neutrophil nonhuman priority journal virus titration Administration, Topical Animals Cornea Disease Progression DNA, Viral Dose-Response Relationship, Drug Enzyme Inhibitors Guanidines Herpesvirus 1, Human Herpesvirus 2, Human Keratitis, Herpetic Mice Mice, Inbred BALB C Nitric Oxide Synthase Ophthalmic Solutions Reverse Transcriptase Polymerase Chain Reaction Trigeminal Ganglion
description	PURPOSE. To study the effect of aminoguanidine (AMG), an inhibitor of nitric oxide production, on the ocular infection of Balb/c mice with herpes simplex virus (HSV) type 1 strain F and HSV-2 strain G. METHODS. Animals were treated with different amounts of AMG (0.5, O. 1, and 0.05 mg/mouse) by topical application in the eye from postinfection (PI) days -2 through +5, considering 0 the day of infection. At different PI days, development of herpetic keratitis was evaluated in treated and control mice. RESULTS. Treated animals showed a dose-dependent increase in ocular disease after viral infection, compared with control animals. Viral titers in ocular washings were higher in AMG-treated mice (PI day 2, HSV-1: AMG 0.5 mg, 1.3 × 103 plaque-forming units (PFU)/ml; control, O. 22 × 102 PFU/ml, P < 0.025). At PI day 3, control corneas had only scattered inflammatory cells, whereas those from treated animals showed a conspicuous infiltrate consisting primarily of neutrophils. Viral titers were also higher in brains of treated mice. These animals died earlier and in a greater proportion than control animals (percentage of mortality, PI day 12, HSV-1: AMG 0.5 mg, 40% ± 4%; control, 18% ± 3%, P < 0.05). CONCLUSIONS. These data indicate an inhibitory effect of nitric oxide on HSV ocular infection.
format	JOUR
author	Benencia, F. Courrèges, M.C. Gamba, G. Cavalieri, H. Massouh, E.J.
author_facet	Benencia, F. Courrèges, M.C. Gamba, G. Cavalieri, H. Massouh, E.J.
author_sort	Benencia, F.
title	Effect of aminoguanidine, a nitric oxide synthase inhibitor, on ocular infection with herpes simplex virus in Balb/c mice
title_short	Effect of aminoguanidine, a nitric oxide synthase inhibitor, on ocular infection with herpes simplex virus in Balb/c mice
title_full	Effect of aminoguanidine, a nitric oxide synthase inhibitor, on ocular infection with herpes simplex virus in Balb/c mice
title_fullStr	Effect of aminoguanidine, a nitric oxide synthase inhibitor, on ocular infection with herpes simplex virus in Balb/c mice
title_full_unstemmed	Effect of aminoguanidine, a nitric oxide synthase inhibitor, on ocular infection with herpes simplex virus in Balb/c mice
title_sort	effect of aminoguanidine, a nitric oxide synthase inhibitor, on ocular infection with herpes simplex virus in balb/c mice
url	http://hdl.handle.net/20.500.12110/paper_01460404_v42_n6_p1277_Benencia
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Effect of aminoguanidine, a nitric oxide synthase inhibitor, on ocular infection with herpes simplex virus in Balb/c mice

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