Hepatic damage and oxidative stress induced by griseofulvin in mice

Erythropoietic Protoporphyria (EPP) is a disease associated with ferrochelatase deficiency, which produces accumulation of protoporphyrin IX (PROTO IX) in erythrocytes, liver and skin. In some cases, a severe hepatic failure and cholestasis was observed. Griseofulvin (Gris) develops an experimental...

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Autores principales:	Martinez, M.D.C., Afonso, S.G., Meiss, R.P., Buzaleh, A.M., Batlle, A.
Formato:	JOUR
Materias:	Erythropoietic Protoporphyria Griseofulvin Heme metabolism Liver damage Oxidative stress 5 aminolevulinate synthase alanine aminotransferase alkaline phosphatase aspartate aminotransferase catalase cytochrome P450 1A2 cytochrome P450 2A6 cytochrome P450 2C9 cytochrome P450 2E1 cytochrome P450 3A4 glutathione glutathione peroxidase glutathione reductase glutathione transferase griseofulvin heme heme oxygenase malonaldehyde protoporphyrin reactive oxygen metabolite superoxide dismutase tryptophan 2,3 dioxygenase alanine aminotransferase blood level alkaline phosphatase blood level animal experiment animal model animal tissue antioxidant activity article aspartate aminotransferase blood level blood level body weight controlled study enzyme activity enzyme induction erythropoietic protoporphyria experimental mouse feces level histopathology liver injury liver level liver weight male mouse nonhuman oxidation reduction reaction oxidative stress Animals Antifungal Agents Aryl Hydrocarbon Hydroxylases Cytochrome P-450 CYP3A Disease Models, Animal Heme Heme Oxygenase (Decyclizing) Immunohistochemistry Liver Male Mice Oxidative Stress Protoporphyria, Erythropoietic Protoporphyrins Tryptophan Oxygenase Animalia Mus
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_01455680_v55_n2_p127_Martinez
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	todo:paper_01455680_v55_n2_p127_Martinez2023-10-03T15:00:07Z Hepatic damage and oxidative stress induced by griseofulvin in mice Martinez, M.D.C. Afonso, S.G. Meiss, R.P. Buzaleh, A.M. Batlle, A. Erythropoietic Protoporphyria Griseofulvin Heme metabolism Liver damage Oxidative stress 5 aminolevulinate synthase alanine aminotransferase alkaline phosphatase aspartate aminotransferase catalase cytochrome P450 1A2 cytochrome P450 2A6 cytochrome P450 2C9 cytochrome P450 2E1 cytochrome P450 3A4 glutathione glutathione peroxidase glutathione reductase glutathione transferase griseofulvin heme heme oxygenase malonaldehyde protoporphyrin reactive oxygen metabolite superoxide dismutase tryptophan 2,3 dioxygenase alanine aminotransferase blood level alkaline phosphatase blood level animal experiment animal model animal tissue antioxidant activity article aspartate aminotransferase blood level blood level body weight controlled study enzyme activity enzyme induction erythropoietic protoporphyria experimental mouse feces level histopathology liver injury liver level liver weight male mouse nonhuman oxidation reduction reaction oxidative stress Animals Antifungal Agents Aryl Hydrocarbon Hydroxylases Cytochrome P-450 CYP3A Disease Models, Animal Griseofulvin Heme Heme Oxygenase (Decyclizing) Immunohistochemistry Liver Male Mice Oxidative Stress Protoporphyria, Erythropoietic Protoporphyrins Tryptophan Oxygenase Animalia Mus Erythropoietic Protoporphyria (EPP) is a disease associated with ferrochelatase deficiency, which produces accumulation of protoporphyrin IX (PROTO IX) in erythrocytes, liver and skin. In some cases, a severe hepatic failure and cholestasis was observed. Griseofulvin (Gris) develops an experimental EPP with hepatic manifestations in animals. The aim of this work was to further characterize this model studying its effect on different metabolisms in mice Gris feeding (0-2.5%, 7 and 14 days). PROTO IX accumulation in liver, blood and feces, induction of ALA-S activity, and a low rate of Holo/Apo tryptophan pyrrolase activity was produced, indicating a reduction of free heme pool. The progressive liver injury was reflected by the aspect and the enlargement of liver and the induction of hepatic damage. Liver redox balance was altered due to porphyrin high concentrations; as a consequence, the antioxidant defense system was disrupted. Heme oxygenase was also induced, however, at higher concentrations of antifungal, the free heme pool would be so depleted that this enzyme would not be necessary. In conclusion, our model of Protoporphyria produced liver alterations similar to those found in EPP patients. Copyright © 2009 C.M.B. Edition. Fil:Martinez, M.D.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Afonso, S.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Buzaleh, A.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Batlle, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01455680_v55_n2_p127_Martinez
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Erythropoietic Protoporphyria Griseofulvin Heme metabolism Liver damage Oxidative stress 5 aminolevulinate synthase alanine aminotransferase alkaline phosphatase aspartate aminotransferase catalase cytochrome P450 1A2 cytochrome P450 2A6 cytochrome P450 2C9 cytochrome P450 2E1 cytochrome P450 3A4 glutathione glutathione peroxidase glutathione reductase glutathione transferase griseofulvin heme heme oxygenase malonaldehyde protoporphyrin reactive oxygen metabolite superoxide dismutase tryptophan 2,3 dioxygenase alanine aminotransferase blood level alkaline phosphatase blood level animal experiment animal model animal tissue antioxidant activity article aspartate aminotransferase blood level blood level body weight controlled study enzyme activity enzyme induction erythropoietic protoporphyria experimental mouse feces level histopathology liver injury liver level liver weight male mouse nonhuman oxidation reduction reaction oxidative stress Animals Antifungal Agents Aryl Hydrocarbon Hydroxylases Cytochrome P-450 CYP3A Disease Models, Animal Griseofulvin Heme Heme Oxygenase (Decyclizing) Immunohistochemistry Liver Male Mice Oxidative Stress Protoporphyria, Erythropoietic Protoporphyrins Tryptophan Oxygenase Animalia Mus
spellingShingle	Erythropoietic Protoporphyria Griseofulvin Heme metabolism Liver damage Oxidative stress 5 aminolevulinate synthase alanine aminotransferase alkaline phosphatase aspartate aminotransferase catalase cytochrome P450 1A2 cytochrome P450 2A6 cytochrome P450 2C9 cytochrome P450 2E1 cytochrome P450 3A4 glutathione glutathione peroxidase glutathione reductase glutathione transferase griseofulvin heme heme oxygenase malonaldehyde protoporphyrin reactive oxygen metabolite superoxide dismutase tryptophan 2,3 dioxygenase alanine aminotransferase blood level alkaline phosphatase blood level animal experiment animal model animal tissue antioxidant activity article aspartate aminotransferase blood level blood level body weight controlled study enzyme activity enzyme induction erythropoietic protoporphyria experimental mouse feces level histopathology liver injury liver level liver weight male mouse nonhuman oxidation reduction reaction oxidative stress Animals Antifungal Agents Aryl Hydrocarbon Hydroxylases Cytochrome P-450 CYP3A Disease Models, Animal Griseofulvin Heme Heme Oxygenase (Decyclizing) Immunohistochemistry Liver Male Mice Oxidative Stress Protoporphyria, Erythropoietic Protoporphyrins Tryptophan Oxygenase Animalia Mus Martinez, M.D.C. Afonso, S.G. Meiss, R.P. Buzaleh, A.M. Batlle, A. Hepatic damage and oxidative stress induced by griseofulvin in mice
topic_facet	Erythropoietic Protoporphyria Griseofulvin Heme metabolism Liver damage Oxidative stress 5 aminolevulinate synthase alanine aminotransferase alkaline phosphatase aspartate aminotransferase catalase cytochrome P450 1A2 cytochrome P450 2A6 cytochrome P450 2C9 cytochrome P450 2E1 cytochrome P450 3A4 glutathione glutathione peroxidase glutathione reductase glutathione transferase griseofulvin heme heme oxygenase malonaldehyde protoporphyrin reactive oxygen metabolite superoxide dismutase tryptophan 2,3 dioxygenase alanine aminotransferase blood level alkaline phosphatase blood level animal experiment animal model animal tissue antioxidant activity article aspartate aminotransferase blood level blood level body weight controlled study enzyme activity enzyme induction erythropoietic protoporphyria experimental mouse feces level histopathology liver injury liver level liver weight male mouse nonhuman oxidation reduction reaction oxidative stress Animals Antifungal Agents Aryl Hydrocarbon Hydroxylases Cytochrome P-450 CYP3A Disease Models, Animal Griseofulvin Heme Heme Oxygenase (Decyclizing) Immunohistochemistry Liver Male Mice Oxidative Stress Protoporphyria, Erythropoietic Protoporphyrins Tryptophan Oxygenase Animalia Mus
description	Erythropoietic Protoporphyria (EPP) is a disease associated with ferrochelatase deficiency, which produces accumulation of protoporphyrin IX (PROTO IX) in erythrocytes, liver and skin. In some cases, a severe hepatic failure and cholestasis was observed. Griseofulvin (Gris) develops an experimental EPP with hepatic manifestations in animals. The aim of this work was to further characterize this model studying its effect on different metabolisms in mice Gris feeding (0-2.5%, 7 and 14 days). PROTO IX accumulation in liver, blood and feces, induction of ALA-S activity, and a low rate of Holo/Apo tryptophan pyrrolase activity was produced, indicating a reduction of free heme pool. The progressive liver injury was reflected by the aspect and the enlargement of liver and the induction of hepatic damage. Liver redox balance was altered due to porphyrin high concentrations; as a consequence, the antioxidant defense system was disrupted. Heme oxygenase was also induced, however, at higher concentrations of antifungal, the free heme pool would be so depleted that this enzyme would not be necessary. In conclusion, our model of Protoporphyria produced liver alterations similar to those found in EPP patients. Copyright © 2009 C.M.B. Edition.
format	JOUR
author	Martinez, M.D.C. Afonso, S.G. Meiss, R.P. Buzaleh, A.M. Batlle, A.
author_facet	Martinez, M.D.C. Afonso, S.G. Meiss, R.P. Buzaleh, A.M. Batlle, A.
author_sort	Martinez, M.D.C.
title	Hepatic damage and oxidative stress induced by griseofulvin in mice
title_short	Hepatic damage and oxidative stress induced by griseofulvin in mice
title_full	Hepatic damage and oxidative stress induced by griseofulvin in mice
title_fullStr	Hepatic damage and oxidative stress induced by griseofulvin in mice
title_full_unstemmed	Hepatic damage and oxidative stress induced by griseofulvin in mice
title_sort	hepatic damage and oxidative stress induced by griseofulvin in mice
url	http://hdl.handle.net/20.500.12110/paper_01455680_v55_n2_p127_Martinez
work_keys_str_mv	AT martinezmdc hepaticdamageandoxidativestressinducedbygriseofulvininmice AT afonsosg hepaticdamageandoxidativestressinducedbygriseofulvininmice AT meissrp hepaticdamageandoxidativestressinducedbygriseofulvininmice AT buzaleham hepaticdamageandoxidativestressinducedbygriseofulvininmice AT batllea hepaticdamageandoxidativestressinducedbygriseofulvininmice
_version_	1807321410611183616

Hepatic damage and oxidative stress induced by griseofulvin in mice

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