An improvement in the dissociating properties of sodium dodecyl sulfate-containing sample buffers used in polyacrylamide gel electrophoresis.
Protein complexes present different degrees of stability. We have previously described a glycoprotein from Bacillus thuringiensis that appeared as a multimer unable to be dissociated by the usual SDS-containing sample buffers of pH 6.8. In order to dissociate the complex, a SDS-containing sample buf...
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| Formato: | JOUR |
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_01455680_v44_n3_p557_GarciaPatrone |
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todo:paper_01455680_v44_n3_p557_GarciaPatrone2023-10-03T15:00:02Z An improvement in the dissociating properties of sodium dodecyl sulfate-containing sample buffers used in polyacrylamide gel electrophoresis. García-Patrone, M. Tandecarz, J.S. bacterial protein buffer dodecyl sulfate sodium article chemistry methodology micelle polyacrylamide gel electrophoresis Bacterial Proteins Buffers Electrophoresis, Polyacrylamide Gel Micelles Sodium Dodecyl Sulfate Protein complexes present different degrees of stability. We have previously described a glycoprotein from Bacillus thuringiensis that appeared as a multimer unable to be dissociated by the usual SDS-containing sample buffers of pH 6.8. In order to dissociate the complex, a SDS-containing sample buffer of pH 9 was described. In the present report three additional protein complexes with different degrees of stability and the effect of that dissociating sample buffer are described. The study of SDS critical micellar concentration values as a function of pH explains the improvement of dissociating properties at pH 9. Fil:Tandecarz, J.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01455680_v44_n3_p557_GarciaPatrone |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
bacterial protein buffer dodecyl sulfate sodium article chemistry methodology micelle polyacrylamide gel electrophoresis Bacterial Proteins Buffers Electrophoresis, Polyacrylamide Gel Micelles Sodium Dodecyl Sulfate |
| spellingShingle |
bacterial protein buffer dodecyl sulfate sodium article chemistry methodology micelle polyacrylamide gel electrophoresis Bacterial Proteins Buffers Electrophoresis, Polyacrylamide Gel Micelles Sodium Dodecyl Sulfate García-Patrone, M. Tandecarz, J.S. An improvement in the dissociating properties of sodium dodecyl sulfate-containing sample buffers used in polyacrylamide gel electrophoresis. |
| topic_facet |
bacterial protein buffer dodecyl sulfate sodium article chemistry methodology micelle polyacrylamide gel electrophoresis Bacterial Proteins Buffers Electrophoresis, Polyacrylamide Gel Micelles Sodium Dodecyl Sulfate |
| description |
Protein complexes present different degrees of stability. We have previously described a glycoprotein from Bacillus thuringiensis that appeared as a multimer unable to be dissociated by the usual SDS-containing sample buffers of pH 6.8. In order to dissociate the complex, a SDS-containing sample buffer of pH 9 was described. In the present report three additional protein complexes with different degrees of stability and the effect of that dissociating sample buffer are described. The study of SDS critical micellar concentration values as a function of pH explains the improvement of dissociating properties at pH 9. |
| format |
JOUR |
| author |
García-Patrone, M. Tandecarz, J.S. |
| author_facet |
García-Patrone, M. Tandecarz, J.S. |
| author_sort |
García-Patrone, M. |
| title |
An improvement in the dissociating properties of sodium dodecyl sulfate-containing sample buffers used in polyacrylamide gel electrophoresis. |
| title_short |
An improvement in the dissociating properties of sodium dodecyl sulfate-containing sample buffers used in polyacrylamide gel electrophoresis. |
| title_full |
An improvement in the dissociating properties of sodium dodecyl sulfate-containing sample buffers used in polyacrylamide gel electrophoresis. |
| title_fullStr |
An improvement in the dissociating properties of sodium dodecyl sulfate-containing sample buffers used in polyacrylamide gel electrophoresis. |
| title_full_unstemmed |
An improvement in the dissociating properties of sodium dodecyl sulfate-containing sample buffers used in polyacrylamide gel electrophoresis. |
| title_sort |
improvement in the dissociating properties of sodium dodecyl sulfate-containing sample buffers used in polyacrylamide gel electrophoresis. |
| url |
http://hdl.handle.net/20.500.12110/paper_01455680_v44_n3_p557_GarciaPatrone |
| work_keys_str_mv |
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1782027714021031936 |