Effects of retinoic acid on a new human erythro-megakaryocytic cell line AP-217

We have characterized a new human cell line AP-217, derived from the peripheral blood of a patient with chronic myeloid leukemia in blastic crisis. The analysis of cell surface antigens and ploidy showed that AP-217 was an erythro-megakaryocytic cell line. The effects of inducers of differentiation...

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Autores principales:	Mossuz, P., Prandini, M.H., Leroux, D., Valiron, O., Schweitzer, A., Sassetti, B., Kolodie, L.
Formato:	JOUR
Materias:	Erythromegakaryocytic cell lines Hb production Leukemic cell differentiation Retinoic acid beta globin globin messenger rna glycophorin a hemoglobin membrane antigen retinoic acid adult article blast cell crisis cell differentiation cell growth cell line cell strain k 562 chronic myeloid leukemia clonogenesis controlled study dose response drug sensitivity erythroid cell globin gene growth inhibition hemoglobin synthesis human human cell leukemia cell male megakaryocyte ploidy priority journal Adult Antigens, Surface Blotting, Northern Cell Differentiation Cell Division Cloning, Molecular Erythrocytes Globins Hemoglobins Humans Isotretinoin Karyotyping Leukemia, Myeloid, Chronic Male Megakaryocytes Polymerase Chain Reaction Tumor Cells, Cultured
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_01452126_v21_n6_p529_Mossuz
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	todo:paper_01452126_v21_n6_p529_Mossuz2023-10-03T14:59:52Z Effects of retinoic acid on a new human erythro-megakaryocytic cell line AP-217 Mossuz, P. Prandini, M.H. Leroux, D. Valiron, O. Schweitzer, A. Sassetti, B. Kolodie, L. Erythromegakaryocytic cell lines Hb production Leukemic cell differentiation Retinoic acid beta globin globin messenger rna glycophorin a hemoglobin membrane antigen retinoic acid adult article blast cell crisis cell differentiation cell growth cell line cell strain k 562 chronic myeloid leukemia clonogenesis controlled study dose response drug sensitivity erythroid cell globin gene growth inhibition hemoglobin synthesis human human cell leukemia cell male megakaryocyte ploidy priority journal Adult Antigens, Surface Blotting, Northern Cell Differentiation Cell Division Cloning, Molecular Erythrocytes Globins Hemoglobins Humans Isotretinoin Karyotyping Leukemia, Myeloid, Chronic Male Megakaryocytes Polymerase Chain Reaction Tumor Cells, Cultured We have characterized a new human cell line AP-217, derived from the peripheral blood of a patient with chronic myeloid leukemia in blastic crisis. The analysis of cell surface antigens and ploidy showed that AP-217 was an erythro-megakaryocytic cell line. The effects of inducers of differentiation were studied and focused on retinoic acid (RA). Uninduced AP-217 cells produced a low level of hemoglobin (Hb) that showed a moderate but significant dose-dependent increase after 13 cis-RA induction (four times above the control at 10-5 M). To outline this effect, AP-217 cells were cloned at limiting dilution. A subclone (clone 2) was isolated which expressed glycophorin A on 12% of cells, and showed a marked sensitivity to RA. After a 4 day induction with increasing concentrations of RA (1-10 x 10-6) Hb production by clone 2 cells was enhanced 12 times over the control at the highest concentration (10-5 M). No effect of RA on the Hb production of K-562 and HEL was observed. This increased Hb production occurred simultaneously with a growth inhibition in clonogenic cultures (20% reduction) associated with a drastic reduction of the colony size. Moreover, we demonstrated the expression of mRNA for the β globin gene in clone 2 and AP-217-cells. This is the first report of a positive effect of RA on the erythroid differentiation of a human leukemic cell line. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01452126_v21_n6_p529_Mossuz
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Erythromegakaryocytic cell lines Hb production Leukemic cell differentiation Retinoic acid beta globin globin messenger rna glycophorin a hemoglobin membrane antigen retinoic acid adult article blast cell crisis cell differentiation cell growth cell line cell strain k 562 chronic myeloid leukemia clonogenesis controlled study dose response drug sensitivity erythroid cell globin gene growth inhibition hemoglobin synthesis human human cell leukemia cell male megakaryocyte ploidy priority journal Adult Antigens, Surface Blotting, Northern Cell Differentiation Cell Division Cloning, Molecular Erythrocytes Globins Hemoglobins Humans Isotretinoin Karyotyping Leukemia, Myeloid, Chronic Male Megakaryocytes Polymerase Chain Reaction Tumor Cells, Cultured
spellingShingle	Erythromegakaryocytic cell lines Hb production Leukemic cell differentiation Retinoic acid beta globin globin messenger rna glycophorin a hemoglobin membrane antigen retinoic acid adult article blast cell crisis cell differentiation cell growth cell line cell strain k 562 chronic myeloid leukemia clonogenesis controlled study dose response drug sensitivity erythroid cell globin gene growth inhibition hemoglobin synthesis human human cell leukemia cell male megakaryocyte ploidy priority journal Adult Antigens, Surface Blotting, Northern Cell Differentiation Cell Division Cloning, Molecular Erythrocytes Globins Hemoglobins Humans Isotretinoin Karyotyping Leukemia, Myeloid, Chronic Male Megakaryocytes Polymerase Chain Reaction Tumor Cells, Cultured Mossuz, P. Prandini, M.H. Leroux, D. Valiron, O. Schweitzer, A. Sassetti, B. Kolodie, L. Effects of retinoic acid on a new human erythro-megakaryocytic cell line AP-217
topic_facet	Erythromegakaryocytic cell lines Hb production Leukemic cell differentiation Retinoic acid beta globin globin messenger rna glycophorin a hemoglobin membrane antigen retinoic acid adult article blast cell crisis cell differentiation cell growth cell line cell strain k 562 chronic myeloid leukemia clonogenesis controlled study dose response drug sensitivity erythroid cell globin gene growth inhibition hemoglobin synthesis human human cell leukemia cell male megakaryocyte ploidy priority journal Adult Antigens, Surface Blotting, Northern Cell Differentiation Cell Division Cloning, Molecular Erythrocytes Globins Hemoglobins Humans Isotretinoin Karyotyping Leukemia, Myeloid, Chronic Male Megakaryocytes Polymerase Chain Reaction Tumor Cells, Cultured
description	We have characterized a new human cell line AP-217, derived from the peripheral blood of a patient with chronic myeloid leukemia in blastic crisis. The analysis of cell surface antigens and ploidy showed that AP-217 was an erythro-megakaryocytic cell line. The effects of inducers of differentiation were studied and focused on retinoic acid (RA). Uninduced AP-217 cells produced a low level of hemoglobin (Hb) that showed a moderate but significant dose-dependent increase after 13 cis-RA induction (four times above the control at 10-5 M). To outline this effect, AP-217 cells were cloned at limiting dilution. A subclone (clone 2) was isolated which expressed glycophorin A on 12% of cells, and showed a marked sensitivity to RA. After a 4 day induction with increasing concentrations of RA (1-10 x 10-6) Hb production by clone 2 cells was enhanced 12 times over the control at the highest concentration (10-5 M). No effect of RA on the Hb production of K-562 and HEL was observed. This increased Hb production occurred simultaneously with a growth inhibition in clonogenic cultures (20% reduction) associated with a drastic reduction of the colony size. Moreover, we demonstrated the expression of mRNA for the β globin gene in clone 2 and AP-217-cells. This is the first report of a positive effect of RA on the erythroid differentiation of a human leukemic cell line.
format	JOUR
author	Mossuz, P. Prandini, M.H. Leroux, D. Valiron, O. Schweitzer, A. Sassetti, B. Kolodie, L.
author_facet	Mossuz, P. Prandini, M.H. Leroux, D. Valiron, O. Schweitzer, A. Sassetti, B. Kolodie, L.
author_sort	Mossuz, P.
title	Effects of retinoic acid on a new human erythro-megakaryocytic cell line AP-217
title_short	Effects of retinoic acid on a new human erythro-megakaryocytic cell line AP-217
title_full	Effects of retinoic acid on a new human erythro-megakaryocytic cell line AP-217
title_fullStr	Effects of retinoic acid on a new human erythro-megakaryocytic cell line AP-217
title_full_unstemmed	Effects of retinoic acid on a new human erythro-megakaryocytic cell line AP-217
title_sort	effects of retinoic acid on a new human erythro-megakaryocytic cell line ap-217
url	http://hdl.handle.net/20.500.12110/paper_01452126_v21_n6_p529_Mossuz
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Effects of retinoic acid on a new human erythro-megakaryocytic cell line AP-217

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