The system of sulfated galactans from the red seaweed Gymnogongrus torulosus (Phyllophoraceae, Rhodophyta): Location and structural analysis

Sulfated polysaccharides were localized in the cuticle, cortex and medulla of the gametophyte thallus, being more concentrated in the intercellular matrix than in the cell walls. During the water extraction sequence, a small percentage of galactan sulfates (5.1% of dry seaweed) with average low Mr (...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Estevez, J.M., Ciancia, M., Cerezo, A.S.
Formato: JOUR
Materias:
Agarans
Carrageenans
Cell wall
Gymnogongrus torulosus (Phyllophoraceae)
l-Galactose
Red seaweed
Sulfated galactans
Algae
Cell membranes
Dialysis
Extraction
Molecular structure
Tissue
Polysaccharides
Gymnogongrus torulosus
Phyllophoraceae
Rhodophyta
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_01448617_v73_n4_p594_Estevez
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_01448617_v73_n4_p594_Estevez
record_format dspace
spelling todo:paper_01448617_v73_n4_p594_Estevez2023-10-03T14:59:47Z The system of sulfated galactans from the red seaweed Gymnogongrus torulosus (Phyllophoraceae, Rhodophyta): Location and structural analysis Estevez, J.M. Ciancia, M. Cerezo, A.S. Agarans Carrageenans Cell wall Gymnogongrus torulosus (Phyllophoraceae) l-Galactose Red seaweed Sulfated galactans Algae Cell membranes Dialysis Extraction Molecular structure Tissue Agarans Carrageenans Gymnogongrus torulosus (Phyllophoraceae) l-Galactose Red seaweed Sulfated galactans Polysaccharides Gymnogongrus torulosus Phyllophoraceae Rhodophyta Sulfated polysaccharides were localized in the cuticle, cortex and medulla of the gametophyte thallus, being more concentrated in the intercellular matrix than in the cell walls. During the water extraction sequence, a small percentage of galactan sulfates (5.1% of dry seaweed) with average low Mr (6-11.4 kDa) were extracted at room temperature without disturbing the cellular arrangement, while sulfated galactans of average medium Mr (18-45 kDa) were obtained by further hot-water extractions (52.4% of dry seaweed), with diorganization of the tissue. The residue (40.0% of dry seaweed) still contained carrageenan-type (major) and agaran-type (minor) galactans. Part of these galactans was extracted with 8.4% LiCl solution in DMSO, from which "pure" κ/ι-carrageenans were isolated. Carrageenans and agarans were extracted in a ratio 1:0.5, showing the highest amount of agaran-structures for a carrageenophyte. The galactans comprise alternating 4-sulfated (major) and non-sulfated (minor) 3-linked β-d-galactopyranose units, and 4-linked α-galactopyranose units with the following substitutions: (i) non-sulfated and 2-sulfated 3,6-anhydro-α-d-galactopyranose residues in the carrageenan-structures, which belong to the κ-family (κ/ι-carrageenans); (ii) 3-sulfated α-l-galactopyranose units and 2-sulfated 3,6-anhydro-α-l-galactopyranose residues in the agaran-structures. Alkaline treatment and alkaline dialysis of the main extracts gave "pure" κ/ι-carrageenans, showing that carrageenan molecules are extracted together with low Mr agarans or agaran-dl-hybrids. © 2008 Elsevier Ltd. All rights reserved. Fil:Estevez, J.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ciancia, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Cerezo, A.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01448617_v73_n4_p594_Estevez
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Agarans
Carrageenans
Cell wall
Gymnogongrus torulosus (Phyllophoraceae)
l-Galactose
Red seaweed
Sulfated galactans
Algae
Cell membranes
Dialysis
Extraction
Molecular structure
Tissue
Agarans
Carrageenans
Gymnogongrus torulosus (Phyllophoraceae)
l-Galactose
Red seaweed
Sulfated galactans
Polysaccharides
Gymnogongrus torulosus
Phyllophoraceae
Rhodophyta
spellingShingle Agarans
Carrageenans
Cell wall
Gymnogongrus torulosus (Phyllophoraceae)
l-Galactose
Red seaweed
Sulfated galactans
Algae
Cell membranes
Dialysis
Extraction
Molecular structure
Tissue
Agarans
Carrageenans
Gymnogongrus torulosus (Phyllophoraceae)
l-Galactose
Red seaweed
Sulfated galactans
Polysaccharides
Gymnogongrus torulosus
Phyllophoraceae
Rhodophyta
Estevez, J.M.
Ciancia, M.
Cerezo, A.S.
The system of sulfated galactans from the red seaweed Gymnogongrus torulosus (Phyllophoraceae, Rhodophyta): Location and structural analysis
topic_facet Agarans
Carrageenans
Cell wall
Gymnogongrus torulosus (Phyllophoraceae)
l-Galactose
Red seaweed
Sulfated galactans
Algae
Cell membranes
Dialysis
Extraction
Molecular structure
Tissue
Agarans
Carrageenans
Gymnogongrus torulosus (Phyllophoraceae)
l-Galactose
Red seaweed
Sulfated galactans
Polysaccharides
Gymnogongrus torulosus
Phyllophoraceae
Rhodophyta
description Sulfated polysaccharides were localized in the cuticle, cortex and medulla of the gametophyte thallus, being more concentrated in the intercellular matrix than in the cell walls. During the water extraction sequence, a small percentage of galactan sulfates (5.1% of dry seaweed) with average low Mr (6-11.4 kDa) were extracted at room temperature without disturbing the cellular arrangement, while sulfated galactans of average medium Mr (18-45 kDa) were obtained by further hot-water extractions (52.4% of dry seaweed), with diorganization of the tissue. The residue (40.0% of dry seaweed) still contained carrageenan-type (major) and agaran-type (minor) galactans. Part of these galactans was extracted with 8.4% LiCl solution in DMSO, from which "pure" κ/ι-carrageenans were isolated. Carrageenans and agarans were extracted in a ratio 1:0.5, showing the highest amount of agaran-structures for a carrageenophyte. The galactans comprise alternating 4-sulfated (major) and non-sulfated (minor) 3-linked β-d-galactopyranose units, and 4-linked α-galactopyranose units with the following substitutions: (i) non-sulfated and 2-sulfated 3,6-anhydro-α-d-galactopyranose residues in the carrageenan-structures, which belong to the κ-family (κ/ι-carrageenans); (ii) 3-sulfated α-l-galactopyranose units and 2-sulfated 3,6-anhydro-α-l-galactopyranose residues in the agaran-structures. Alkaline treatment and alkaline dialysis of the main extracts gave "pure" κ/ι-carrageenans, showing that carrageenan molecules are extracted together with low Mr agarans or agaran-dl-hybrids. © 2008 Elsevier Ltd. All rights reserved.
format JOUR
author Estevez, J.M.
Ciancia, M.
Cerezo, A.S.
author_facet Estevez, J.M.
Ciancia, M.
Cerezo, A.S.
author_sort Estevez, J.M.
title The system of sulfated galactans from the red seaweed Gymnogongrus torulosus (Phyllophoraceae, Rhodophyta): Location and structural analysis
title_short The system of sulfated galactans from the red seaweed Gymnogongrus torulosus (Phyllophoraceae, Rhodophyta): Location and structural analysis
title_full The system of sulfated galactans from the red seaweed Gymnogongrus torulosus (Phyllophoraceae, Rhodophyta): Location and structural analysis
title_fullStr The system of sulfated galactans from the red seaweed Gymnogongrus torulosus (Phyllophoraceae, Rhodophyta): Location and structural analysis
title_full_unstemmed The system of sulfated galactans from the red seaweed Gymnogongrus torulosus (Phyllophoraceae, Rhodophyta): Location and structural analysis
title_sort system of sulfated galactans from the red seaweed gymnogongrus torulosus (phyllophoraceae, rhodophyta): location and structural analysis
url http://hdl.handle.net/20.500.12110/paper_01448617_v73_n4_p594_Estevez
work_keys_str_mv AT estevezjm thesystemofsulfatedgalactansfromtheredseaweedgymnogongrustorulosusphyllophoraceaerhodophytalocationandstructuralanalysis
AT cianciam thesystemofsulfatedgalactansfromtheredseaweedgymnogongrustorulosusphyllophoraceaerhodophytalocationandstructuralanalysis
AT cerezoas thesystemofsulfatedgalactansfromtheredseaweedgymnogongrustorulosusphyllophoraceaerhodophytalocationandstructuralanalysis
AT estevezjm systemofsulfatedgalactansfromtheredseaweedgymnogongrustorulosusphyllophoraceaerhodophytalocationandstructuralanalysis
AT cianciam systemofsulfatedgalactansfromtheredseaweedgymnogongrustorulosusphyllophoraceaerhodophytalocationandstructuralanalysis
AT cerezoas systemofsulfatedgalactansfromtheredseaweedgymnogongrustorulosusphyllophoraceaerhodophytalocationandstructuralanalysis
_version_ 1782028376770347008

Ejemplares similares