The alkali-soluble galactomannan-like oligosaccharides from the seed of Gleditsia triacanthos
Extraction with alkali of the seeds of Gleditsia triacanthos, after exhaustive extraction with water and neutralization of the extracts by dialysis, permits isolation of the 75% ethanol-soluble products. Gel permeation chromatography of these products allows isolation of an homogeneous galactomannan...
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todo:paper_01448617_v6_n3_p203_Mazzini2023-10-03T14:59:46Z The alkali-soluble galactomannan-like oligosaccharides from the seed of Gleditsia triacanthos Mazzini, M.N. Cerezo, A.S. Extraction with alkali of the seeds of Gleditsia triacanthos, after exhaustive extraction with water and neutralization of the extracts by dialysis, permits isolation of the 75% ethanol-soluble products. Gel permeation chromatography of these products allows isolation of an homogeneous galactomannan-protein complex which was further purified by anion-exchange chromatography. The galactomannan has a medium degree of polymerization and a backbone similar to that of the classical galactomannans. The main difference with these polysaccharides lies in the side-chains which are attached to the backbone not only by (1 → 6)-linkages but also by (1 → 3)-linkages and which contain, on average, two galactose units, either (1 → 3), (1 → 6)-linked or (1 → 3), (1 → 3)-linked with α-d and β-d configurations, respectively. © 1986. Fil:Mazzini, M.N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Cerezo, A.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01448617_v6_n3_p203_Mazzini |
institution |
Universidad de Buenos Aires |
institution_str |
I-28 |
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R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
description |
Extraction with alkali of the seeds of Gleditsia triacanthos, after exhaustive extraction with water and neutralization of the extracts by dialysis, permits isolation of the 75% ethanol-soluble products. Gel permeation chromatography of these products allows isolation of an homogeneous galactomannan-protein complex which was further purified by anion-exchange chromatography. The galactomannan has a medium degree of polymerization and a backbone similar to that of the classical galactomannans. The main difference with these polysaccharides lies in the side-chains which are attached to the backbone not only by (1 → 6)-linkages but also by (1 → 3)-linkages and which contain, on average, two galactose units, either (1 → 3), (1 → 6)-linked or (1 → 3), (1 → 3)-linked with α-d and β-d configurations, respectively. © 1986. |
format |
JOUR |
author |
Mazzini, M.N. Cerezo, A.S. |
spellingShingle |
Mazzini, M.N. Cerezo, A.S. The alkali-soluble galactomannan-like oligosaccharides from the seed of Gleditsia triacanthos |
author_facet |
Mazzini, M.N. Cerezo, A.S. |
author_sort |
Mazzini, M.N. |
title |
The alkali-soluble galactomannan-like oligosaccharides from the seed of Gleditsia triacanthos |
title_short |
The alkali-soluble galactomannan-like oligosaccharides from the seed of Gleditsia triacanthos |
title_full |
The alkali-soluble galactomannan-like oligosaccharides from the seed of Gleditsia triacanthos |
title_fullStr |
The alkali-soluble galactomannan-like oligosaccharides from the seed of Gleditsia triacanthos |
title_full_unstemmed |
The alkali-soluble galactomannan-like oligosaccharides from the seed of Gleditsia triacanthos |
title_sort |
alkali-soluble galactomannan-like oligosaccharides from the seed of gleditsia triacanthos |
url |
http://hdl.handle.net/20.500.12110/paper_01448617_v6_n3_p203_Mazzini |
work_keys_str_mv |
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1782029934618738688 |