Gums induced microstructure stability in Ca(II)-alginate beads containing lactase analyzed by SAXS

Previous works show that the addition of trehalose and gums in β-galactosidase (lactase) Ca(II)-alginate encapsulation systems improved its intrinsic stability against freezing and dehydration processes in the pristine state. However, there is no available information on the evolution in microstruct...

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Autores principales:	Traffano-Schiffo, M.V., Castro-Giraldez, M., Fito, P.J., Perullini, M., Santagapita, P.R.
Formato:	JOUR
Materias:	Alginate beads Encapsulation Hydrocolloids Microstructure Small-Angle X-ray scattering (SAXS) β-Galactosidase Calcium Dehydration X ray scattering Alginate encapsulation Galactosidases Microstructural changes Microstructure stability Operational conditions Structural modifications Alginate alginic acid excipient galactan glucuronic acid guar gum gum arabic hexuronic acid immobilized enzyme lactase mannan nitrobenzene derivative plant gum trehalose chemistry diffusion hydrogel metabolism small angle scattering time factor X ray diffraction Alginates Diffusion Enzymes, Immobilized Excipients Galactans Glucuronic Acid Gum Arabic Hexuronic Acids Hydrogels Lactase Mannans Nitrobenzenes Plant Gums Scattering, Small Angle Time Factors Trehalose X-Ray Diffraction
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_01448617_v179_n_p402_TraffanoSchiffo
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	todo:paper_01448617_v179_n_p402_TraffanoSchiffo2023-10-03T14:59:39Z Gums induced microstructure stability in Ca(II)-alginate beads containing lactase analyzed by SAXS Traffano-Schiffo, M.V. Castro-Giraldez, M. Fito, P.J. Perullini, M. Santagapita, P.R. Alginate beads Encapsulation Hydrocolloids Microstructure Small-Angle X-ray scattering (SAXS) β-Galactosidase Calcium Dehydration Encapsulation Microstructure X ray scattering Alginate beads Alginate encapsulation Galactosidases Hydrocolloids Microstructural changes Microstructure stability Operational conditions Structural modifications Alginate alginic acid excipient galactan glucuronic acid guar gum gum arabic hexuronic acid immobilized enzyme lactase mannan nitrobenzene derivative plant gum trehalose chemistry diffusion hydrogel metabolism small angle scattering time factor X ray diffraction Alginates Diffusion Enzymes, Immobilized Excipients Galactans Glucuronic Acid Gum Arabic Hexuronic Acids Hydrogels Lactase Mannans Nitrobenzenes Plant Gums Scattering, Small Angle Time Factors Trehalose X-Ray Diffraction Previous works show that the addition of trehalose and gums in β-galactosidase (lactase) Ca(II)-alginate encapsulation systems improved its intrinsic stability against freezing and dehydration processes in the pristine state. However, there is no available information on the evolution in microstructure due to the constraints imposed by the operational conditions. The aim of this research is to study the time course of microstructural changes of Ca(II)-alginate matrices driven by the presence of trehalose, arabic and guar gums as excipients and to discuss how these changes influence the diffusional transport (assessed by LF-NMR) and the enzymatic activity of the encapsulated lactase. The structural modifications at different scales were assessed by SAXS. The incorporation of gums as second excipients induces a significant stabilization in the microstructure not only at the rod scale, but also in the characteristic size and density of alginate dimers (basic units of construction of rods) and the degree of interconnection of rods at a larger scale, improving the performance in terms of lactase activity. © 2017 Elsevier Ltd JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01448617_v179_n_p402_TraffanoSchiffo
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Alginate beads Encapsulation Hydrocolloids Microstructure Small-Angle X-ray scattering (SAXS) β-Galactosidase Calcium Dehydration Encapsulation Microstructure X ray scattering Alginate beads Alginate encapsulation Galactosidases Hydrocolloids Microstructural changes Microstructure stability Operational conditions Structural modifications Alginate alginic acid excipient galactan glucuronic acid guar gum gum arabic hexuronic acid immobilized enzyme lactase mannan nitrobenzene derivative plant gum trehalose chemistry diffusion hydrogel metabolism small angle scattering time factor X ray diffraction Alginates Diffusion Enzymes, Immobilized Excipients Galactans Glucuronic Acid Gum Arabic Hexuronic Acids Hydrogels Lactase Mannans Nitrobenzenes Plant Gums Scattering, Small Angle Time Factors Trehalose X-Ray Diffraction
spellingShingle	Alginate beads Encapsulation Hydrocolloids Microstructure Small-Angle X-ray scattering (SAXS) β-Galactosidase Calcium Dehydration Encapsulation Microstructure X ray scattering Alginate beads Alginate encapsulation Galactosidases Hydrocolloids Microstructural changes Microstructure stability Operational conditions Structural modifications Alginate alginic acid excipient galactan glucuronic acid guar gum gum arabic hexuronic acid immobilized enzyme lactase mannan nitrobenzene derivative plant gum trehalose chemistry diffusion hydrogel metabolism small angle scattering time factor X ray diffraction Alginates Diffusion Enzymes, Immobilized Excipients Galactans Glucuronic Acid Gum Arabic Hexuronic Acids Hydrogels Lactase Mannans Nitrobenzenes Plant Gums Scattering, Small Angle Time Factors Trehalose X-Ray Diffraction Traffano-Schiffo, M.V. Castro-Giraldez, M. Fito, P.J. Perullini, M. Santagapita, P.R. Gums induced microstructure stability in Ca(II)-alginate beads containing lactase analyzed by SAXS
topic_facet	Alginate beads Encapsulation Hydrocolloids Microstructure Small-Angle X-ray scattering (SAXS) β-Galactosidase Calcium Dehydration Encapsulation Microstructure X ray scattering Alginate beads Alginate encapsulation Galactosidases Hydrocolloids Microstructural changes Microstructure stability Operational conditions Structural modifications Alginate alginic acid excipient galactan glucuronic acid guar gum gum arabic hexuronic acid immobilized enzyme lactase mannan nitrobenzene derivative plant gum trehalose chemistry diffusion hydrogel metabolism small angle scattering time factor X ray diffraction Alginates Diffusion Enzymes, Immobilized Excipients Galactans Glucuronic Acid Gum Arabic Hexuronic Acids Hydrogels Lactase Mannans Nitrobenzenes Plant Gums Scattering, Small Angle Time Factors Trehalose X-Ray Diffraction
description	Previous works show that the addition of trehalose and gums in β-galactosidase (lactase) Ca(II)-alginate encapsulation systems improved its intrinsic stability against freezing and dehydration processes in the pristine state. However, there is no available information on the evolution in microstructure due to the constraints imposed by the operational conditions. The aim of this research is to study the time course of microstructural changes of Ca(II)-alginate matrices driven by the presence of trehalose, arabic and guar gums as excipients and to discuss how these changes influence the diffusional transport (assessed by LF-NMR) and the enzymatic activity of the encapsulated lactase. The structural modifications at different scales were assessed by SAXS. The incorporation of gums as second excipients induces a significant stabilization in the microstructure not only at the rod scale, but also in the characteristic size and density of alginate dimers (basic units of construction of rods) and the degree of interconnection of rods at a larger scale, improving the performance in terms of lactase activity. © 2017 Elsevier Ltd
format	JOUR
author	Traffano-Schiffo, M.V. Castro-Giraldez, M. Fito, P.J. Perullini, M. Santagapita, P.R.
author_facet	Traffano-Schiffo, M.V. Castro-Giraldez, M. Fito, P.J. Perullini, M. Santagapita, P.R.
author_sort	Traffano-Schiffo, M.V.
title	Gums induced microstructure stability in Ca(II)-alginate beads containing lactase analyzed by SAXS
title_short	Gums induced microstructure stability in Ca(II)-alginate beads containing lactase analyzed by SAXS
title_full	Gums induced microstructure stability in Ca(II)-alginate beads containing lactase analyzed by SAXS
title_fullStr	Gums induced microstructure stability in Ca(II)-alginate beads containing lactase analyzed by SAXS
title_full_unstemmed	Gums induced microstructure stability in Ca(II)-alginate beads containing lactase analyzed by SAXS
title_sort	gums induced microstructure stability in ca(ii)-alginate beads containing lactase analyzed by saxs
url	http://hdl.handle.net/20.500.12110/paper_01448617_v179_n_p402_TraffanoSchiffo
work_keys_str_mv	AT traffanoschiffomv gumsinducedmicrostructurestabilityincaiialginatebeadscontaininglactaseanalyzedbysaxs AT castrogiraldezm gumsinducedmicrostructurestabilityincaiialginatebeadscontaininglactaseanalyzedbysaxs AT fitopj gumsinducedmicrostructurestabilityincaiialginatebeadscontaininglactaseanalyzedbysaxs AT perullinim gumsinducedmicrostructurestabilityincaiialginatebeadscontaininglactaseanalyzedbysaxs AT santagapitapr gumsinducedmicrostructurestabilityincaiialginatebeadscontaininglactaseanalyzedbysaxs
_version_	1782026545739595776

Gums induced microstructure stability in Ca(II)-alginate beads containing lactase analyzed by SAXS

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