Minor polysaccharidic constituents from the red seaweed Hypnea musciformis. Appearance of a novel branched uronic acid

Two polysaccharide fractions isolated from Hypnea musciformis after room temperature- and hot water extraction, soluble after KCl precipitation of the more abundant carrageenans, were subfractionated by ion-exchange chromatography eluting with increasing concentrations of NaCl. The lowest NaCl conce...

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Autores principales:	Cosenza, V.A., Navarro, D.A., Stortz, C.A.
Formato:	JOUR
Materias:	3-C-carboxy-D-erythrofuranose Agarans Apiuronic acid Carrageenans Hypnea musciformis Chromatographic analysis Ion chromatography Ion exchange Hot water extraction Ion exchange chromatography Kappaphycus alvarezii NaCl concentration Chromatography carrageenan polysaccharide uronic acid chemistry isolation and purification red alga seaweed Carrageenan Polysaccharides Rhodophyta Seaweed Uronic Acids
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_01448617_v157_n_p156_Cosenza
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_01448617_v157_n_p156_Cosenza
record_format	dspace
spelling	todo:paper_01448617_v157_n_p156_Cosenza2023-10-03T14:59:37Z Minor polysaccharidic constituents from the red seaweed Hypnea musciformis. Appearance of a novel branched uronic acid Cosenza, V.A. Navarro, D.A. Stortz, C.A. 3-C-carboxy-D-erythrofuranose Agarans Apiuronic acid Carrageenans Hypnea musciformis Chromatographic analysis Ion chromatography Ion exchange 3-C-carboxy-D-erythrofuranose Agarans Carrageenans Hot water extraction Hypnea musciformis Ion exchange chromatography Kappaphycus alvarezii NaCl concentration Chromatography carrageenan polysaccharide uronic acid chemistry isolation and purification red alga seaweed Carrageenan Polysaccharides Rhodophyta Seaweed Uronic Acids Two polysaccharide fractions isolated from Hypnea musciformis after room temperature- and hot water extraction, soluble after KCl precipitation of the more abundant carrageenans, were subfractionated by ion-exchange chromatography eluting with increasing concentrations of NaCl. The lowest NaCl concentration (0.2 M) eluted agarans. The DL-hybrids (or mixtures) eluted at intermediate concentrations of NaCl. The D/L-galactose ratio and the sulfate proportion increased with the NaCl concentration. Different types of substitution were present, mainly at C-3 with sulfate, Xyl and methylated Gal stubs, as well as low amounts of 3,6-AnGal. A novel constituent, identified as 3-C-carboxy-D-erythrose1 in its β-furanosic form, was found linked to C-6 of β-Gal units. A search carried out in other species like Iridaea undulosa and Kappaphycus alvarezii also revealed the same constituent. Finally, the late-eluting fractions were mostly carrageenans, with a structure consistent with that of a κ/ι/ν-carrageenan hybrid. © 2016 Elsevier Ltd Fil:Navarro, D.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Stortz, C.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01448617_v157_n_p156_Cosenza
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	3-C-carboxy-D-erythrofuranose Agarans Apiuronic acid Carrageenans Hypnea musciformis Chromatographic analysis Ion chromatography Ion exchange 3-C-carboxy-D-erythrofuranose Agarans Carrageenans Hot water extraction Hypnea musciformis Ion exchange chromatography Kappaphycus alvarezii NaCl concentration Chromatography carrageenan polysaccharide uronic acid chemistry isolation and purification red alga seaweed Carrageenan Polysaccharides Rhodophyta Seaweed Uronic Acids
spellingShingle	3-C-carboxy-D-erythrofuranose Agarans Apiuronic acid Carrageenans Hypnea musciformis Chromatographic analysis Ion chromatography Ion exchange 3-C-carboxy-D-erythrofuranose Agarans Carrageenans Hot water extraction Hypnea musciformis Ion exchange chromatography Kappaphycus alvarezii NaCl concentration Chromatography carrageenan polysaccharide uronic acid chemistry isolation and purification red alga seaweed Carrageenan Polysaccharides Rhodophyta Seaweed Uronic Acids Cosenza, V.A. Navarro, D.A. Stortz, C.A. Minor polysaccharidic constituents from the red seaweed Hypnea musciformis. Appearance of a novel branched uronic acid
topic_facet	3-C-carboxy-D-erythrofuranose Agarans Apiuronic acid Carrageenans Hypnea musciformis Chromatographic analysis Ion chromatography Ion exchange 3-C-carboxy-D-erythrofuranose Agarans Carrageenans Hot water extraction Hypnea musciformis Ion exchange chromatography Kappaphycus alvarezii NaCl concentration Chromatography carrageenan polysaccharide uronic acid chemistry isolation and purification red alga seaweed Carrageenan Polysaccharides Rhodophyta Seaweed Uronic Acids
description	Two polysaccharide fractions isolated from Hypnea musciformis after room temperature- and hot water extraction, soluble after KCl precipitation of the more abundant carrageenans, were subfractionated by ion-exchange chromatography eluting with increasing concentrations of NaCl. The lowest NaCl concentration (0.2 M) eluted agarans. The DL-hybrids (or mixtures) eluted at intermediate concentrations of NaCl. The D/L-galactose ratio and the sulfate proportion increased with the NaCl concentration. Different types of substitution were present, mainly at C-3 with sulfate, Xyl and methylated Gal stubs, as well as low amounts of 3,6-AnGal. A novel constituent, identified as 3-C-carboxy-D-erythrose1 in its β-furanosic form, was found linked to C-6 of β-Gal units. A search carried out in other species like Iridaea undulosa and Kappaphycus alvarezii also revealed the same constituent. Finally, the late-eluting fractions were mostly carrageenans, with a structure consistent with that of a κ/ι/ν-carrageenan hybrid. © 2016 Elsevier Ltd
format	JOUR
author	Cosenza, V.A. Navarro, D.A. Stortz, C.A.
author_facet	Cosenza, V.A. Navarro, D.A. Stortz, C.A.
author_sort	Cosenza, V.A.
title	Minor polysaccharidic constituents from the red seaweed Hypnea musciformis. Appearance of a novel branched uronic acid
title_short	Minor polysaccharidic constituents from the red seaweed Hypnea musciformis. Appearance of a novel branched uronic acid
title_full	Minor polysaccharidic constituents from the red seaweed Hypnea musciformis. Appearance of a novel branched uronic acid
title_fullStr	Minor polysaccharidic constituents from the red seaweed Hypnea musciformis. Appearance of a novel branched uronic acid
title_full_unstemmed	Minor polysaccharidic constituents from the red seaweed Hypnea musciformis. Appearance of a novel branched uronic acid
title_sort	minor polysaccharidic constituents from the red seaweed hypnea musciformis. appearance of a novel branched uronic acid
url	http://hdl.handle.net/20.500.12110/paper_01448617_v157_n_p156_Cosenza
work_keys_str_mv	AT cosenzava minorpolysaccharidicconstituentsfromtheredseaweedhypneamusciformisappearanceofanovelbrancheduronicacid AT navarroda minorpolysaccharidicconstituentsfromtheredseaweedhypneamusciformisappearanceofanovelbrancheduronicacid AT stortzca minorpolysaccharidicconstituentsfromtheredseaweedhypneamusciformisappearanceofanovelbrancheduronicacid
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Minor polysaccharidic constituents from the red seaweed Hypnea musciformis. Appearance of a novel branched uronic acid

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