Novel sulfated xylogalactoarabinans from green seaweed Cladophora falklandica: Chemical structure and action on the fibrin network
The water-soluble sulfated xylogalactoarabinans from green seaweed Cladophora falklandica are constituted by a backbone of 4-linked β-L-arabinopyranose units partially sulfated mainly on C3 and also on C2. Besides, partial glycosylation mostly on C2 with single stubs of β-D-xylopyranose, or single s...
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todo:paper_01448617_v154_n_p139_Arata2023-10-03T14:59:37Z Novel sulfated xylogalactoarabinans from green seaweed Cladophora falklandica: Chemical structure and action on the fibrin network Arata, P.X. Quintana, I. Raffo, M.P. Ciancia, M. Anticoagulant activity Cladophora Fibrin network Galactofuranose Green seaweed Sulfated arabinan Blood vessels Scanning electron microscopy Anticoagulant activities Arabinans Cladophora Fibrin network Galactofuranose Green seaweed Seaweed anticoagulant agent fibrin polysaccharide sulfate xylopyranose xylose analogs and derivatives animal chemistry conformation drug effects fibrinolysis green alga human Leporidae metabolism preclinical study procedures scanning electron microscopy seaweed Animals Anticoagulants Carbohydrate Conformation Chlorophyta Drug Evaluation, Preclinical Fibrin Fibrinolysis Humans Microscopy, Electron, Scanning Polysaccharides Rabbits Seaweed Sulfates Xylose The water-soluble sulfated xylogalactoarabinans from green seaweed Cladophora falklandica are constituted by a backbone of 4-linked β-L-arabinopyranose units partially sulfated mainly on C3 and also on C2. Besides, partial glycosylation mostly on C2 with single stubs of β-D-xylopyranose, or single stubs of β-D-galactofuranose or short chains comprising (1 → 5)- and/or (1 → 6)-linkages, was also found. These compounds showed anticoagulant activity, although much lower than that of heparin. The effect of a purified fraction (F1) on the fibrin network was studied in detail. It modifies the kinetics of fibrin formation, suggesting an impaired polymerization process. Scanning electron microscopy showed a laxer conformation, with larger interstitial pores than the control. Accordingly, this network was lysed more easily. These fibrin properties would reduce the time of permanence of the clot in the blood vessel, inducing a lesser thrombogenic state. One of the possible mechanisms of its anticoagulant effect is direct thrombin inhibition. © 2016 Elsevier Ltd Fil:Quintana, I. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ciancia, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01448617_v154_n_p139_Arata |
institution |
Universidad de Buenos Aires |
institution_str |
I-28 |
repository_str |
R-134 |
collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
topic |
Anticoagulant activity Cladophora Fibrin network Galactofuranose Green seaweed Sulfated arabinan Blood vessels Scanning electron microscopy Anticoagulant activities Arabinans Cladophora Fibrin network Galactofuranose Green seaweed Seaweed anticoagulant agent fibrin polysaccharide sulfate xylopyranose xylose analogs and derivatives animal chemistry conformation drug effects fibrinolysis green alga human Leporidae metabolism preclinical study procedures scanning electron microscopy seaweed Animals Anticoagulants Carbohydrate Conformation Chlorophyta Drug Evaluation, Preclinical Fibrin Fibrinolysis Humans Microscopy, Electron, Scanning Polysaccharides Rabbits Seaweed Sulfates Xylose |
spellingShingle |
Anticoagulant activity Cladophora Fibrin network Galactofuranose Green seaweed Sulfated arabinan Blood vessels Scanning electron microscopy Anticoagulant activities Arabinans Cladophora Fibrin network Galactofuranose Green seaweed Seaweed anticoagulant agent fibrin polysaccharide sulfate xylopyranose xylose analogs and derivatives animal chemistry conformation drug effects fibrinolysis green alga human Leporidae metabolism preclinical study procedures scanning electron microscopy seaweed Animals Anticoagulants Carbohydrate Conformation Chlorophyta Drug Evaluation, Preclinical Fibrin Fibrinolysis Humans Microscopy, Electron, Scanning Polysaccharides Rabbits Seaweed Sulfates Xylose Arata, P.X. Quintana, I. Raffo, M.P. Ciancia, M. Novel sulfated xylogalactoarabinans from green seaweed Cladophora falklandica: Chemical structure and action on the fibrin network |
topic_facet |
Anticoagulant activity Cladophora Fibrin network Galactofuranose Green seaweed Sulfated arabinan Blood vessels Scanning electron microscopy Anticoagulant activities Arabinans Cladophora Fibrin network Galactofuranose Green seaweed Seaweed anticoagulant agent fibrin polysaccharide sulfate xylopyranose xylose analogs and derivatives animal chemistry conformation drug effects fibrinolysis green alga human Leporidae metabolism preclinical study procedures scanning electron microscopy seaweed Animals Anticoagulants Carbohydrate Conformation Chlorophyta Drug Evaluation, Preclinical Fibrin Fibrinolysis Humans Microscopy, Electron, Scanning Polysaccharides Rabbits Seaweed Sulfates Xylose |
description |
The water-soluble sulfated xylogalactoarabinans from green seaweed Cladophora falklandica are constituted by a backbone of 4-linked β-L-arabinopyranose units partially sulfated mainly on C3 and also on C2. Besides, partial glycosylation mostly on C2 with single stubs of β-D-xylopyranose, or single stubs of β-D-galactofuranose or short chains comprising (1 → 5)- and/or (1 → 6)-linkages, was also found. These compounds showed anticoagulant activity, although much lower than that of heparin. The effect of a purified fraction (F1) on the fibrin network was studied in detail. It modifies the kinetics of fibrin formation, suggesting an impaired polymerization process. Scanning electron microscopy showed a laxer conformation, with larger interstitial pores than the control. Accordingly, this network was lysed more easily. These fibrin properties would reduce the time of permanence of the clot in the blood vessel, inducing a lesser thrombogenic state. One of the possible mechanisms of its anticoagulant effect is direct thrombin inhibition. © 2016 Elsevier Ltd |
format |
JOUR |
author |
Arata, P.X. Quintana, I. Raffo, M.P. Ciancia, M. |
author_facet |
Arata, P.X. Quintana, I. Raffo, M.P. Ciancia, M. |
author_sort |
Arata, P.X. |
title |
Novel sulfated xylogalactoarabinans from green seaweed Cladophora falklandica: Chemical structure and action on the fibrin network |
title_short |
Novel sulfated xylogalactoarabinans from green seaweed Cladophora falklandica: Chemical structure and action on the fibrin network |
title_full |
Novel sulfated xylogalactoarabinans from green seaweed Cladophora falklandica: Chemical structure and action on the fibrin network |
title_fullStr |
Novel sulfated xylogalactoarabinans from green seaweed Cladophora falklandica: Chemical structure and action on the fibrin network |
title_full_unstemmed |
Novel sulfated xylogalactoarabinans from green seaweed Cladophora falklandica: Chemical structure and action on the fibrin network |
title_sort |
novel sulfated xylogalactoarabinans from green seaweed cladophora falklandica: chemical structure and action on the fibrin network |
url |
http://hdl.handle.net/20.500.12110/paper_01448617_v154_n_p139_Arata |
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