Retraction of rod-like mitochondria during microtubule-dependent transport

Molecular motors play relevant roles on the regulation of mitochondria size and shape, essential properties for the cell homeostasis. In this work, we tracked single rod-shaped mitochondria with nanometer precision to explore the performance of microtubule motor teams during processive anterograde a...

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Autores principales:	De Rossi, M.C., Levi, V., Bruno, L.
Formato:	JOUR
Materias:	dynein adenosine triphosphatase enhanced green fluorescent protein kinesin animal cell Article cell organelle cell size cell transport confocal microscopy homeostasis immortalized cell line intracellular transport melanophore microtubule mitochondrion molecular imaging nonhuman Xenopus laevis animal genetics metabolism organelle shape single cell analysis Animals Dyneins Homeostasis Kinesin Microtubules Mitochondria Organelle Shape Single-Cell Analysis
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_01448463_v38_n3_p_DeRossi
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_01448463_v38_n3_p_DeRossi
record_format	dspace
spelling	todo:paper_01448463_v38_n3_p_DeRossi2023-10-03T14:59:31Z Retraction of rod-like mitochondria during microtubule-dependent transport De Rossi, M.C. Levi, V. Bruno, L. dynein adenosine triphosphatase enhanced green fluorescent protein kinesin dynein adenosine triphosphatase kinesin animal cell Article cell organelle cell size cell transport confocal microscopy homeostasis immortalized cell line intracellular transport melanophore microtubule mitochondrion molecular imaging nonhuman Xenopus laevis animal genetics metabolism microtubule mitochondrion organelle shape single cell analysis Animals Dyneins Homeostasis Kinesin Microtubules Mitochondria Organelle Shape Single-Cell Analysis Xenopus laevis Molecular motors play relevant roles on the regulation of mitochondria size and shape, essential properties for the cell homeostasis. In this work, we tracked single rod-shaped mitochondria with nanometer precision to explore the performance of microtubule motor teams during processive anterograde and retrograde transport. We analyzed simultaneously the organelle size and verified that mitochondria retracted during retrograde transport with their leading tip moving slower in comparison with the rear tip. In contrast, mitochondria preserved their size during anterograde runs indicating a different performance of plus-end directed teams. These results were interpreted considering the different performance of dynein and kinesin teams and provide valuable information on the collective action of motors during mitochondria transport. © 2018 The Author(s). JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01448463_v38_n3_p_DeRossi
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	dynein adenosine triphosphatase enhanced green fluorescent protein kinesin dynein adenosine triphosphatase kinesin animal cell Article cell organelle cell size cell transport confocal microscopy homeostasis immortalized cell line intracellular transport melanophore microtubule mitochondrion molecular imaging nonhuman Xenopus laevis animal genetics metabolism microtubule mitochondrion organelle shape single cell analysis Animals Dyneins Homeostasis Kinesin Microtubules Mitochondria Organelle Shape Single-Cell Analysis Xenopus laevis
spellingShingle	dynein adenosine triphosphatase enhanced green fluorescent protein kinesin dynein adenosine triphosphatase kinesin animal cell Article cell organelle cell size cell transport confocal microscopy homeostasis immortalized cell line intracellular transport melanophore microtubule mitochondrion molecular imaging nonhuman Xenopus laevis animal genetics metabolism microtubule mitochondrion organelle shape single cell analysis Animals Dyneins Homeostasis Kinesin Microtubules Mitochondria Organelle Shape Single-Cell Analysis Xenopus laevis De Rossi, M.C. Levi, V. Bruno, L. Retraction of rod-like mitochondria during microtubule-dependent transport
topic_facet	dynein adenosine triphosphatase enhanced green fluorescent protein kinesin dynein adenosine triphosphatase kinesin animal cell Article cell organelle cell size cell transport confocal microscopy homeostasis immortalized cell line intracellular transport melanophore microtubule mitochondrion molecular imaging nonhuman Xenopus laevis animal genetics metabolism microtubule mitochondrion organelle shape single cell analysis Animals Dyneins Homeostasis Kinesin Microtubules Mitochondria Organelle Shape Single-Cell Analysis Xenopus laevis
description	Molecular motors play relevant roles on the regulation of mitochondria size and shape, essential properties for the cell homeostasis. In this work, we tracked single rod-shaped mitochondria with nanometer precision to explore the performance of microtubule motor teams during processive anterograde and retrograde transport. We analyzed simultaneously the organelle size and verified that mitochondria retracted during retrograde transport with their leading tip moving slower in comparison with the rear tip. In contrast, mitochondria preserved their size during anterograde runs indicating a different performance of plus-end directed teams. These results were interpreted considering the different performance of dynein and kinesin teams and provide valuable information on the collective action of motors during mitochondria transport. © 2018 The Author(s).
format	JOUR
author	De Rossi, M.C. Levi, V. Bruno, L.
author_facet	De Rossi, M.C. Levi, V. Bruno, L.
author_sort	De Rossi, M.C.
title	Retraction of rod-like mitochondria during microtubule-dependent transport
title_short	Retraction of rod-like mitochondria during microtubule-dependent transport
title_full	Retraction of rod-like mitochondria during microtubule-dependent transport
title_fullStr	Retraction of rod-like mitochondria during microtubule-dependent transport
title_full_unstemmed	Retraction of rod-like mitochondria during microtubule-dependent transport
title_sort	retraction of rod-like mitochondria during microtubule-dependent transport
url	http://hdl.handle.net/20.500.12110/paper_01448463_v38_n3_p_DeRossi
work_keys_str_mv	AT derossimc retractionofrodlikemitochondriaduringmicrotubuledependenttransport AT leviv retractionofrodlikemitochondriaduringmicrotubuledependenttransport AT brunol retractionofrodlikemitochondriaduringmicrotubuledependenttransport
_version_	1782027334099927040

Retraction of rod-like mitochondria during microtubule-dependent transport

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