In situ prey selection of mixotrophic and heterotrophic flagellates in Antarctic oligotrophic lakes: An analysis of the digestive vacuole content

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We investigated the selective predation of mixotrophic and heterotrophic flagellates (MF and HF) on different heterotrophic prokaryote phylotypes (HPP; Bacteria + Archaea) living in natural assemblages from oligotrophic Antarctic lakes. In situ prey preference was analyzed for the first time on diff...

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Detalles Bibliográficos
Autores principales: Gerea, M., Queimaliños, C., Schiaffino, M.R., Izaguirre, I., Forn, I., Massana, R., Unrein, F.
Formato: JOUR
Materias:
CARD-FISH
Heterotrophic flagellates
Mixotrophic flagellates
Oligotrophic lakes
Prey selection
catalysis
digestive system
flagellate
heterotrophy
lake ecosystem
mixotrophy
morphology
oligotrophic environment
phylogenetics
phytoplankton
predation
prey selection
prokaryote
protist
Antarctica
Actinobacteria
Alphaproteobacteria
Archaea
Bacteria (microorganisms)
Bacteroidetes
Betaproteobacteria
Chrysophyceae
Mastigophora (flagellates)
Ochromonas
Prokaryota
Protista
Pseudopedinella
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_01427873_v35_n1_p201_Gerea
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_01427873_v35_n1_p201_Gerea
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spelling todo:paper_01427873_v35_n1_p201_Gerea2023-10-03T14:59:02Z In situ prey selection of mixotrophic and heterotrophic flagellates in Antarctic oligotrophic lakes: An analysis of the digestive vacuole content Gerea, M. Queimaliños, C. Schiaffino, M.R. Izaguirre, I. Forn, I. Massana, R. Unrein, F. CARD-FISH Heterotrophic flagellates Mixotrophic flagellates Oligotrophic lakes Prey selection catalysis digestive system flagellate heterotrophy lake ecosystem mixotrophy morphology oligotrophic environment phylogenetics phytoplankton predation prey selection prokaryote protist Antarctica Actinobacteria Alphaproteobacteria Archaea Bacteria (microorganisms) Bacteroidetes Betaproteobacteria Chrysophyceae Mastigophora (flagellates) Ochromonas Prokaryota Protista Pseudopedinella We investigated the selective predation of mixotrophic and heterotrophic flagellates (MF and HF) on different heterotrophic prokaryote phylotypes (HPP; Bacteria + Archaea) living in natural assemblages from oligotrophic Antarctic lakes. In situ prey preference was analyzed for the first time on different mixotrophic taxa (Pseudopedinella sp., Ochromonas-like cells, Chrysophyceae >5 μm). The relative abundances of seven different HPP hybridized by CARD-FISH (catalyzed reporter deposition-fluorescent in situ hybridization) in natural community were compared with the proportions of hybridized cells inside digestive vacuoles. Our results showed some general trends to selectivity over some HPP. Alphaproteobacteria and Betaproteobacteria were the most abundant groups, and strikingly, a negative selection trend was detected in most samples by all bacterivorous protists. In contrast, for Actinobacteria a positive selection trend was observed in most samples, whereas Bacteroidetes seemed to be randomly preyed upon. Interestingly, similar prey preferences were observed in all bacterivorous flagellates. Our results suggest that phylogenetic affiliation determines part of the process of prey selection by protists in these lakes. Nevertheless, other features, such as cell size, morphology and the presence of the S-layer, might also significantly contribute to prey selectivity on the HPP. © 2012 The Author 2012. Published by Oxford University Press. All rights reserved. Fil:Queimaliños, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Schiaffino, M.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Izaguirre, I. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Unrein, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01427873_v35_n1_p201_Gerea
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic CARD-FISH
Heterotrophic flagellates
Mixotrophic flagellates
Oligotrophic lakes
Prey selection
catalysis
digestive system
flagellate
heterotrophy
lake ecosystem
mixotrophy
morphology
oligotrophic environment
phylogenetics
phytoplankton
predation
prey selection
prokaryote
protist
Antarctica
Actinobacteria
Alphaproteobacteria
Archaea
Bacteria (microorganisms)
Bacteroidetes
Betaproteobacteria
Chrysophyceae
Mastigophora (flagellates)
Ochromonas
Prokaryota
Protista
Pseudopedinella
spellingShingle CARD-FISH
Heterotrophic flagellates
Mixotrophic flagellates
Oligotrophic lakes
Prey selection
catalysis
digestive system
flagellate
heterotrophy
lake ecosystem
mixotrophy
morphology
oligotrophic environment
phylogenetics
phytoplankton
predation
prey selection
prokaryote
protist
Antarctica
Actinobacteria
Alphaproteobacteria
Archaea
Bacteria (microorganisms)
Bacteroidetes
Betaproteobacteria
Chrysophyceae
Mastigophora (flagellates)
Ochromonas
Prokaryota
Protista
Pseudopedinella
Gerea, M.
Queimaliños, C.
Schiaffino, M.R.
Izaguirre, I.
Forn, I.
Massana, R.
Unrein, F.
In situ prey selection of mixotrophic and heterotrophic flagellates in Antarctic oligotrophic lakes: An analysis of the digestive vacuole content
topic_facet CARD-FISH
Heterotrophic flagellates
Mixotrophic flagellates
Oligotrophic lakes
Prey selection
catalysis
digestive system
flagellate
heterotrophy
lake ecosystem
mixotrophy
morphology
oligotrophic environment
phylogenetics
phytoplankton
predation
prey selection
prokaryote
protist
Antarctica
Actinobacteria
Alphaproteobacteria
Archaea
Bacteria (microorganisms)
Bacteroidetes
Betaproteobacteria
Chrysophyceae
Mastigophora (flagellates)
Ochromonas
Prokaryota
Protista
Pseudopedinella
description We investigated the selective predation of mixotrophic and heterotrophic flagellates (MF and HF) on different heterotrophic prokaryote phylotypes (HPP; Bacteria + Archaea) living in natural assemblages from oligotrophic Antarctic lakes. In situ prey preference was analyzed for the first time on different mixotrophic taxa (Pseudopedinella sp., Ochromonas-like cells, Chrysophyceae >5 μm). The relative abundances of seven different HPP hybridized by CARD-FISH (catalyzed reporter deposition-fluorescent in situ hybridization) in natural community were compared with the proportions of hybridized cells inside digestive vacuoles. Our results showed some general trends to selectivity over some HPP. Alphaproteobacteria and Betaproteobacteria were the most abundant groups, and strikingly, a negative selection trend was detected in most samples by all bacterivorous protists. In contrast, for Actinobacteria a positive selection trend was observed in most samples, whereas Bacteroidetes seemed to be randomly preyed upon. Interestingly, similar prey preferences were observed in all bacterivorous flagellates. Our results suggest that phylogenetic affiliation determines part of the process of prey selection by protists in these lakes. Nevertheless, other features, such as cell size, morphology and the presence of the S-layer, might also significantly contribute to prey selectivity on the HPP. © 2012 The Author 2012. Published by Oxford University Press. All rights reserved.
format JOUR
author Gerea, M.
Queimaliños, C.
Schiaffino, M.R.
Izaguirre, I.
Forn, I.
Massana, R.
Unrein, F.
author_facet Gerea, M.
Queimaliños, C.
Schiaffino, M.R.
Izaguirre, I.
Forn, I.
Massana, R.
Unrein, F.
author_sort Gerea, M.
title In situ prey selection of mixotrophic and heterotrophic flagellates in Antarctic oligotrophic lakes: An analysis of the digestive vacuole content
title_short In situ prey selection of mixotrophic and heterotrophic flagellates in Antarctic oligotrophic lakes: An analysis of the digestive vacuole content
title_full In situ prey selection of mixotrophic and heterotrophic flagellates in Antarctic oligotrophic lakes: An analysis of the digestive vacuole content
title_fullStr In situ prey selection of mixotrophic and heterotrophic flagellates in Antarctic oligotrophic lakes: An analysis of the digestive vacuole content
title_full_unstemmed In situ prey selection of mixotrophic and heterotrophic flagellates in Antarctic oligotrophic lakes: An analysis of the digestive vacuole content
title_sort in situ prey selection of mixotrophic and heterotrophic flagellates in antarctic oligotrophic lakes: an analysis of the digestive vacuole content
url http://hdl.handle.net/20.500.12110/paper_01427873_v35_n1_p201_Gerea
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