Structural and immunological characterization of sulphatides: Relevance of sulphate moieties in Trypanosoma cruzi glycoconjugates
Sulphoglycosphingolipids, present on the surface of diverse cells, participate in the regulation of various cellular events. However, little is known about the structure and the role of sulphoglycosphingolipids in trypanosomatids. Herein, sulphated dihexosylceramide structures - composed mainly of s...
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todo:paper_01419838_v34_n11_p499_Acosta2023-10-03T14:58:50Z Structural and immunological characterization of sulphatides: Relevance of sulphate moieties in Trypanosoma cruzi glycoconjugates Acosta, D.M. Soprano, L.L. Ferrero, M.R. Esteva, M.I. Riarte, A. Couto, A.S. Duschak, V.G. Cruzipain antibodies Sulphate moieties Sulphatides Trypanosoma cruzi UV-MALDI-TOF-MS ceramide cruzipain cysteine proteinase epitope glycoconjugate glycolipid immunoglobulin G2 sphingosine stearic acid sulfate sulfatide sulphated dihexosylceramide unclassified drug animal experiment animal model article carboxy terminal sequence Chagas disease chemical structure clinical article controlled study cross reaction epimastigote human matrix assisted laser desorption ionization time of flight mass spectrometry nonhuman priority journal rabbit structure analysis Trypanosoma cruzi ultraviolet radiation Adult Animals Antiprotozoal Agents Chagas Disease Cross Reactions Cysteine Endopeptidases Enzyme-Linked Immunosorbent Assay Female Glycoconjugates Humans Immunoglobulin G Male Middle Aged Rabbits Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Sulfoglycosphingolipids Trypanosoma cruzi Sulphoglycosphingolipids, present on the surface of diverse cells, participate in the regulation of various cellular events. However, little is known about the structure and the role of sulphoglycosphingolipids in trypanosomatids. Herein, sulphated dihexosylceramide structures - composed mainly of sphingosine as the long chain base acylated with stearic acid - have been determined for the first time in Trypanosoma cruzi epimastigotes by UV-MALDI-TOF-MS analysis. Interestingly, inhibition ELISA assays using cruzipain as antigen and polyclonal rabbit antibodies specific for cruzipain, the major cysteine proteinase of T. cruzi, or for its C-terminal domain, have demonstrated (i) that sulphate epitopes are shared between cruzipain and sulphatides of T. cruzi, (ii) that cross-reactivity maps to the C-terminal domain and (iii) the existence of other antigenic determinants in the glycolipidic structures. These features provide evidence that sulphate groups are antigenic in sulphate-containing parasite glycoconjugates. Furthermore, IgG2 antibody levels inversely correlate with disease severity in chronic Chagas disease patients, suggesting that IgG2 antibodies specific for sulphated epitopes might be associated with protective immunity and might be considered as potential surrogates of the course of chronic Chagas disease. © 2012 Blackwell Publishing Ltd. Fil:Couto, A.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01419838_v34_n11_p499_Acosta |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Cruzipain antibodies Sulphate moieties Sulphatides Trypanosoma cruzi UV-MALDI-TOF-MS ceramide cruzipain cysteine proteinase epitope glycoconjugate glycolipid immunoglobulin G2 sphingosine stearic acid sulfate sulfatide sulphated dihexosylceramide unclassified drug animal experiment animal model article carboxy terminal sequence Chagas disease chemical structure clinical article controlled study cross reaction epimastigote human matrix assisted laser desorption ionization time of flight mass spectrometry nonhuman priority journal rabbit structure analysis Trypanosoma cruzi ultraviolet radiation Adult Animals Antiprotozoal Agents Chagas Disease Cross Reactions Cysteine Endopeptidases Enzyme-Linked Immunosorbent Assay Female Glycoconjugates Humans Immunoglobulin G Male Middle Aged Rabbits Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Sulfoglycosphingolipids Trypanosoma cruzi |
| spellingShingle |
Cruzipain antibodies Sulphate moieties Sulphatides Trypanosoma cruzi UV-MALDI-TOF-MS ceramide cruzipain cysteine proteinase epitope glycoconjugate glycolipid immunoglobulin G2 sphingosine stearic acid sulfate sulfatide sulphated dihexosylceramide unclassified drug animal experiment animal model article carboxy terminal sequence Chagas disease chemical structure clinical article controlled study cross reaction epimastigote human matrix assisted laser desorption ionization time of flight mass spectrometry nonhuman priority journal rabbit structure analysis Trypanosoma cruzi ultraviolet radiation Adult Animals Antiprotozoal Agents Chagas Disease Cross Reactions Cysteine Endopeptidases Enzyme-Linked Immunosorbent Assay Female Glycoconjugates Humans Immunoglobulin G Male Middle Aged Rabbits Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Sulfoglycosphingolipids Trypanosoma cruzi Acosta, D.M. Soprano, L.L. Ferrero, M.R. Esteva, M.I. Riarte, A. Couto, A.S. Duschak, V.G. Structural and immunological characterization of sulphatides: Relevance of sulphate moieties in Trypanosoma cruzi glycoconjugates |
| topic_facet |
Cruzipain antibodies Sulphate moieties Sulphatides Trypanosoma cruzi UV-MALDI-TOF-MS ceramide cruzipain cysteine proteinase epitope glycoconjugate glycolipid immunoglobulin G2 sphingosine stearic acid sulfate sulfatide sulphated dihexosylceramide unclassified drug animal experiment animal model article carboxy terminal sequence Chagas disease chemical structure clinical article controlled study cross reaction epimastigote human matrix assisted laser desorption ionization time of flight mass spectrometry nonhuman priority journal rabbit structure analysis Trypanosoma cruzi ultraviolet radiation Adult Animals Antiprotozoal Agents Chagas Disease Cross Reactions Cysteine Endopeptidases Enzyme-Linked Immunosorbent Assay Female Glycoconjugates Humans Immunoglobulin G Male Middle Aged Rabbits Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Sulfoglycosphingolipids Trypanosoma cruzi |
| description |
Sulphoglycosphingolipids, present on the surface of diverse cells, participate in the regulation of various cellular events. However, little is known about the structure and the role of sulphoglycosphingolipids in trypanosomatids. Herein, sulphated dihexosylceramide structures - composed mainly of sphingosine as the long chain base acylated with stearic acid - have been determined for the first time in Trypanosoma cruzi epimastigotes by UV-MALDI-TOF-MS analysis. Interestingly, inhibition ELISA assays using cruzipain as antigen and polyclonal rabbit antibodies specific for cruzipain, the major cysteine proteinase of T. cruzi, or for its C-terminal domain, have demonstrated (i) that sulphate epitopes are shared between cruzipain and sulphatides of T. cruzi, (ii) that cross-reactivity maps to the C-terminal domain and (iii) the existence of other antigenic determinants in the glycolipidic structures. These features provide evidence that sulphate groups are antigenic in sulphate-containing parasite glycoconjugates. Furthermore, IgG2 antibody levels inversely correlate with disease severity in chronic Chagas disease patients, suggesting that IgG2 antibodies specific for sulphated epitopes might be associated with protective immunity and might be considered as potential surrogates of the course of chronic Chagas disease. © 2012 Blackwell Publishing Ltd. |
| format |
JOUR |
| author |
Acosta, D.M. Soprano, L.L. Ferrero, M.R. Esteva, M.I. Riarte, A. Couto, A.S. Duschak, V.G. |
| author_facet |
Acosta, D.M. Soprano, L.L. Ferrero, M.R. Esteva, M.I. Riarte, A. Couto, A.S. Duschak, V.G. |
| author_sort |
Acosta, D.M. |
| title |
Structural and immunological characterization of sulphatides: Relevance of sulphate moieties in Trypanosoma cruzi glycoconjugates |
| title_short |
Structural and immunological characterization of sulphatides: Relevance of sulphate moieties in Trypanosoma cruzi glycoconjugates |
| title_full |
Structural and immunological characterization of sulphatides: Relevance of sulphate moieties in Trypanosoma cruzi glycoconjugates |
| title_fullStr |
Structural and immunological characterization of sulphatides: Relevance of sulphate moieties in Trypanosoma cruzi glycoconjugates |
| title_full_unstemmed |
Structural and immunological characterization of sulphatides: Relevance of sulphate moieties in Trypanosoma cruzi glycoconjugates |
| title_sort |
structural and immunological characterization of sulphatides: relevance of sulphate moieties in trypanosoma cruzi glycoconjugates |
| url |
http://hdl.handle.net/20.500.12110/paper_01419838_v34_n11_p499_Acosta |
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