Xylans from Scinaia hatei: Structural features, sulfation and anti-HSV activity

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Natural compounds offer interesting pharmacological perspectives for antiviral drug development with regard to broad-spectrum antiviral properties and novel modes of action. In this study, we have analyzed alkali-extracted xylan of Scinaia hatei. Alkali extraction of this red alga yielded a xylan sh...

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Autores principales:	Mandal, P., Pujol, C.A., Damonte, E.B., Ghosh, T., Ray, B.
Formato:	JOUR
Materias:	Antiviral activity Herpes simplex virus Liagoraceae Scinaia hatei Sulfation Xylan acetone antivirus agent petroleum ether polysaccharide sulfate xylan animal cell antiviral activity article chemical bond controlled study drug cytotoxicity drug structure gel permeation chromatography glycosidic linkage Herpes simplex virus 1 Herpes simplex virus 2 macromolecule molecular weight nonhuman proton nuclear magnetic resonance seaweed solvent extraction Soxhlet extraction structure activity relation sulfation virion virus inactivation Algae, Red Animals Antiviral Agents Cercopithecus aethiops Chromatography, Gel Glycosides Herpesvirus 1, Human Herpesvirus 2, Human Magnetic Resonance Spectroscopy Molecular Weight Sulfur Vero Cells Xylans Rhodophyta Scinaia Simplexvirus
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_01418130_v46_n2_p173_Mandal
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_01418130_v46_n2_p173_Mandal
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spelling	todo:paper_01418130_v46_n2_p173_Mandal2023-10-03T14:58:47Z Xylans from Scinaia hatei: Structural features, sulfation and anti-HSV activity Mandal, P. Pujol, C.A. Damonte, E.B. Ghosh, T. Ray, B. Antiviral activity Herpes simplex virus Liagoraceae Scinaia hatei Sulfation Xylan acetone antivirus agent petroleum ether polysaccharide sulfate xylan animal cell antiviral activity article chemical bond controlled study drug cytotoxicity drug structure gel permeation chromatography glycosidic linkage Herpes simplex virus Herpes simplex virus 1 Herpes simplex virus 2 macromolecule molecular weight nonhuman proton nuclear magnetic resonance Scinaia hatei seaweed solvent extraction Soxhlet extraction structure activity relation sulfation virion virus inactivation Algae, Red Animals Antiviral Agents Cercopithecus aethiops Chromatography, Gel Glycosides Herpesvirus 1, Human Herpesvirus 2, Human Magnetic Resonance Spectroscopy Molecular Weight Sulfur Vero Cells Xylans Liagoraceae Rhodophyta Scinaia Simplexvirus Natural compounds offer interesting pharmacological perspectives for antiviral drug development with regard to broad-spectrum antiviral properties and novel modes of action. In this study, we have analyzed alkali-extracted xylan of Scinaia hatei. Alkali extraction of this red alga yielded a xylan shown to have a molecular mass of 120kDa and a linear structure of (1→4)-linked β-d-xylopyranosyl residues. Derivatives (S1, S2, S3 and S4) generated by chemical sulfation from this macromolecule had degree of sulfation between 0.93 and 1.95, and contained strong anti-HSV activity with inhibitory concentration 50% (IC50) from 0.22 to 1.37μg/ml. Furthermore, they had no direct inactivating effect on virions in a virucidal assay. Sulfate groups account for their in vitro antiviral activity. Interestingly, sulfated xylans already exerted anti-HSV activity when only pre-incubated with the cultured cells prior to infection, thus pointing to a main inhibitory effect on viral entry. © 2009 Elsevier B.V. Fil:Pujol, C.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Damonte, E.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01418130_v46_n2_p173_Mandal
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Antiviral activity Herpes simplex virus Liagoraceae Scinaia hatei Sulfation Xylan acetone antivirus agent petroleum ether polysaccharide sulfate xylan animal cell antiviral activity article chemical bond controlled study drug cytotoxicity drug structure gel permeation chromatography glycosidic linkage Herpes simplex virus Herpes simplex virus 1 Herpes simplex virus 2 macromolecule molecular weight nonhuman proton nuclear magnetic resonance Scinaia hatei seaweed solvent extraction Soxhlet extraction structure activity relation sulfation virion virus inactivation Algae, Red Animals Antiviral Agents Cercopithecus aethiops Chromatography, Gel Glycosides Herpesvirus 1, Human Herpesvirus 2, Human Magnetic Resonance Spectroscopy Molecular Weight Sulfur Vero Cells Xylans Liagoraceae Rhodophyta Scinaia Simplexvirus
spellingShingle	Antiviral activity Herpes simplex virus Liagoraceae Scinaia hatei Sulfation Xylan acetone antivirus agent petroleum ether polysaccharide sulfate xylan animal cell antiviral activity article chemical bond controlled study drug cytotoxicity drug structure gel permeation chromatography glycosidic linkage Herpes simplex virus Herpes simplex virus 1 Herpes simplex virus 2 macromolecule molecular weight nonhuman proton nuclear magnetic resonance Scinaia hatei seaweed solvent extraction Soxhlet extraction structure activity relation sulfation virion virus inactivation Algae, Red Animals Antiviral Agents Cercopithecus aethiops Chromatography, Gel Glycosides Herpesvirus 1, Human Herpesvirus 2, Human Magnetic Resonance Spectroscopy Molecular Weight Sulfur Vero Cells Xylans Liagoraceae Rhodophyta Scinaia Simplexvirus Mandal, P. Pujol, C.A. Damonte, E.B. Ghosh, T. Ray, B. Xylans from Scinaia hatei: Structural features, sulfation and anti-HSV activity
topic_facet	Antiviral activity Herpes simplex virus Liagoraceae Scinaia hatei Sulfation Xylan acetone antivirus agent petroleum ether polysaccharide sulfate xylan animal cell antiviral activity article chemical bond controlled study drug cytotoxicity drug structure gel permeation chromatography glycosidic linkage Herpes simplex virus Herpes simplex virus 1 Herpes simplex virus 2 macromolecule molecular weight nonhuman proton nuclear magnetic resonance Scinaia hatei seaweed solvent extraction Soxhlet extraction structure activity relation sulfation virion virus inactivation Algae, Red Animals Antiviral Agents Cercopithecus aethiops Chromatography, Gel Glycosides Herpesvirus 1, Human Herpesvirus 2, Human Magnetic Resonance Spectroscopy Molecular Weight Sulfur Vero Cells Xylans Liagoraceae Rhodophyta Scinaia Simplexvirus
description	Natural compounds offer interesting pharmacological perspectives for antiviral drug development with regard to broad-spectrum antiviral properties and novel modes of action. In this study, we have analyzed alkali-extracted xylan of Scinaia hatei. Alkali extraction of this red alga yielded a xylan shown to have a molecular mass of 120kDa and a linear structure of (1→4)-linked β-d-xylopyranosyl residues. Derivatives (S1, S2, S3 and S4) generated by chemical sulfation from this macromolecule had degree of sulfation between 0.93 and 1.95, and contained strong anti-HSV activity with inhibitory concentration 50% (IC50) from 0.22 to 1.37μg/ml. Furthermore, they had no direct inactivating effect on virions in a virucidal assay. Sulfate groups account for their in vitro antiviral activity. Interestingly, sulfated xylans already exerted anti-HSV activity when only pre-incubated with the cultured cells prior to infection, thus pointing to a main inhibitory effect on viral entry. © 2009 Elsevier B.V.
format	JOUR
author	Mandal, P. Pujol, C.A. Damonte, E.B. Ghosh, T. Ray, B.
author_facet	Mandal, P. Pujol, C.A. Damonte, E.B. Ghosh, T. Ray, B.
author_sort	Mandal, P.
title	Xylans from Scinaia hatei: Structural features, sulfation and anti-HSV activity
title_short	Xylans from Scinaia hatei: Structural features, sulfation and anti-HSV activity
title_full	Xylans from Scinaia hatei: Structural features, sulfation and anti-HSV activity
title_fullStr	Xylans from Scinaia hatei: Structural features, sulfation and anti-HSV activity
title_full_unstemmed	Xylans from Scinaia hatei: Structural features, sulfation and anti-HSV activity
title_sort	xylans from scinaia hatei: structural features, sulfation and anti-hsv activity
url	http://hdl.handle.net/20.500.12110/paper_01418130_v46_n2_p173_Mandal
work_keys_str_mv	AT mandalp xylansfromscinaiahateistructuralfeaturessulfationandantihsvactivity AT pujolca xylansfromscinaiahateistructuralfeaturessulfationandantihsvactivity AT damonteeb xylansfromscinaiahateistructuralfeaturessulfationandantihsvactivity AT ghosht xylansfromscinaiahateistructuralfeaturessulfationandantihsvactivity AT rayb xylansfromscinaiahateistructuralfeaturessulfationandantihsvactivity
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Xylans from Scinaia hatei: Structural features, sulfation and anti-HSV activity

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