Anti-herpes simplex virus activity of sulfated galactans from the red seaweeds Gymnogongrus griffithsiae and Cryptonemia crenulata
This study presents the chemical composition and antiviral activity against herpes simplex virus type 1 (HSV-1) and 2 (HSV-2) of sulfated galactan crude extracts and main fractions obtained from two red seaweeds collected in Brazil, Gymnogongrus griffithsiae and Cryptonemia crenulata. Most of the ei...
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_01418130_v34_n1-2_p63_Talarico |
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todo:paper_01418130_v34_n1-2_p63_Talarico2023-10-03T14:58:45Z Anti-herpes simplex virus activity of sulfated galactans from the red seaweeds Gymnogongrus griffithsiae and Cryptonemia crenulata Talarico, L.B. Zibetti, R.G.M. Faria, P.C.S. Scolaro, L.A. Duarte, M.E.R. Noseda, M.D. Pujol, C.A. Damonte, E.B. Antiviral activity D,L-Galactan hybrid Herpes simplex virus Kappa/iota/nu carrageenan Red seaweed Sulfated polysaccharide carrageenan galactan animal cell animal experiment animal model antiviral activity article Brazil cell assay chemical composition controlled study cryptonemia crenulata cytotoxicity drug activity drug effect drug mechanism drug screening female gymnogongrus griffithsiae Herpes simplex virus 1 Herpes simplex virus 2 hybrid IC 50 inhibition kinetics mouse nonhuman protection seaweed sulfation vaginitis Vero cell virion virus adsorption virus inactivation virus plaque Animals Antiviral Agents Brazil Cercopithecus aethiops Drug Evaluation, Preclinical Female Galactans Herpes Simplex Herpesvirus 1, Human Herpesvirus 2, Human Inhibitory Concentration 50 Mice Mice, Inbred BALB C Seaweed Simplexvirus Sulfates Toxicity Tests Vaginal Diseases Vero Cells algae Cryptonemia Cryptonemia crenulata Gymnogongrus Gymnogongrus griffithsiae Human herpesvirus 1 Human herpesvirus 2 Murinae Simplexvirus This study presents the chemical composition and antiviral activity against herpes simplex virus type 1 (HSV-1) and 2 (HSV-2) of sulfated galactan crude extracts and main fractions obtained from two red seaweeds collected in Brazil, Gymnogongrus griffithsiae and Cryptonemia crenulata. Most of the eighteen tested products, including homogeneous kappa/iota/nu carrageenan and DL-galactan hybrid, exhibited antiherpetic activity with inhibitory concentration 50% (IC50) values in the range 0.5-5.6 μg/ml, as determined in a virus plaque reduction assay in Vero cells. The galactans lacked cytotoxic effects and showed a broad spectrum of antiviral activity against HSV-1 and HSV-2. No direct virus inactivation was observed after virion treatment with the galactans. The mode of action of these compounds could be mainly ascribed to an inhibitory effect on virus adsorption. Most importantly, a significant protection against a murine vaginal infection with HSV-2 was afforded by topical treatment with the sulfated galactans. © 2004 Elsevier B.V. All rights reserved. Fil:Talarico, L.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Scolaro, L.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Noseda, M.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pujol, C.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Damonte, E.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01418130_v34_n1-2_p63_Talarico |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Antiviral activity D,L-Galactan hybrid Herpes simplex virus Kappa/iota/nu carrageenan Red seaweed Sulfated polysaccharide carrageenan galactan animal cell animal experiment animal model antiviral activity article Brazil cell assay chemical composition controlled study cryptonemia crenulata cytotoxicity drug activity drug effect drug mechanism drug screening female gymnogongrus griffithsiae Herpes simplex virus 1 Herpes simplex virus 2 hybrid IC 50 inhibition kinetics mouse nonhuman protection seaweed sulfation vaginitis Vero cell virion virus adsorption virus inactivation virus plaque Animals Antiviral Agents Brazil Cercopithecus aethiops Drug Evaluation, Preclinical Female Galactans Herpes Simplex Herpesvirus 1, Human Herpesvirus 2, Human Inhibitory Concentration 50 Mice Mice, Inbred BALB C Seaweed Simplexvirus Sulfates Toxicity Tests Vaginal Diseases Vero Cells algae Cryptonemia Cryptonemia crenulata Gymnogongrus Gymnogongrus griffithsiae Human herpesvirus 1 Human herpesvirus 2 Murinae Simplexvirus |
| spellingShingle |
Antiviral activity D,L-Galactan hybrid Herpes simplex virus Kappa/iota/nu carrageenan Red seaweed Sulfated polysaccharide carrageenan galactan animal cell animal experiment animal model antiviral activity article Brazil cell assay chemical composition controlled study cryptonemia crenulata cytotoxicity drug activity drug effect drug mechanism drug screening female gymnogongrus griffithsiae Herpes simplex virus 1 Herpes simplex virus 2 hybrid IC 50 inhibition kinetics mouse nonhuman protection seaweed sulfation vaginitis Vero cell virion virus adsorption virus inactivation virus plaque Animals Antiviral Agents Brazil Cercopithecus aethiops Drug Evaluation, Preclinical Female Galactans Herpes Simplex Herpesvirus 1, Human Herpesvirus 2, Human Inhibitory Concentration 50 Mice Mice, Inbred BALB C Seaweed Simplexvirus Sulfates Toxicity Tests Vaginal Diseases Vero Cells algae Cryptonemia Cryptonemia crenulata Gymnogongrus Gymnogongrus griffithsiae Human herpesvirus 1 Human herpesvirus 2 Murinae Simplexvirus Talarico, L.B. Zibetti, R.G.M. Faria, P.C.S. Scolaro, L.A. Duarte, M.E.R. Noseda, M.D. Pujol, C.A. Damonte, E.B. Anti-herpes simplex virus activity of sulfated galactans from the red seaweeds Gymnogongrus griffithsiae and Cryptonemia crenulata |
| topic_facet |
Antiviral activity D,L-Galactan hybrid Herpes simplex virus Kappa/iota/nu carrageenan Red seaweed Sulfated polysaccharide carrageenan galactan animal cell animal experiment animal model antiviral activity article Brazil cell assay chemical composition controlled study cryptonemia crenulata cytotoxicity drug activity drug effect drug mechanism drug screening female gymnogongrus griffithsiae Herpes simplex virus 1 Herpes simplex virus 2 hybrid IC 50 inhibition kinetics mouse nonhuman protection seaweed sulfation vaginitis Vero cell virion virus adsorption virus inactivation virus plaque Animals Antiviral Agents Brazil Cercopithecus aethiops Drug Evaluation, Preclinical Female Galactans Herpes Simplex Herpesvirus 1, Human Herpesvirus 2, Human Inhibitory Concentration 50 Mice Mice, Inbred BALB C Seaweed Simplexvirus Sulfates Toxicity Tests Vaginal Diseases Vero Cells algae Cryptonemia Cryptonemia crenulata Gymnogongrus Gymnogongrus griffithsiae Human herpesvirus 1 Human herpesvirus 2 Murinae Simplexvirus |
| description |
This study presents the chemical composition and antiviral activity against herpes simplex virus type 1 (HSV-1) and 2 (HSV-2) of sulfated galactan crude extracts and main fractions obtained from two red seaweeds collected in Brazil, Gymnogongrus griffithsiae and Cryptonemia crenulata. Most of the eighteen tested products, including homogeneous kappa/iota/nu carrageenan and DL-galactan hybrid, exhibited antiherpetic activity with inhibitory concentration 50% (IC50) values in the range 0.5-5.6 μg/ml, as determined in a virus plaque reduction assay in Vero cells. The galactans lacked cytotoxic effects and showed a broad spectrum of antiviral activity against HSV-1 and HSV-2. No direct virus inactivation was observed after virion treatment with the galactans. The mode of action of these compounds could be mainly ascribed to an inhibitory effect on virus adsorption. Most importantly, a significant protection against a murine vaginal infection with HSV-2 was afforded by topical treatment with the sulfated galactans. © 2004 Elsevier B.V. All rights reserved. |
| format |
JOUR |
| author |
Talarico, L.B. Zibetti, R.G.M. Faria, P.C.S. Scolaro, L.A. Duarte, M.E.R. Noseda, M.D. Pujol, C.A. Damonte, E.B. |
| author_facet |
Talarico, L.B. Zibetti, R.G.M. Faria, P.C.S. Scolaro, L.A. Duarte, M.E.R. Noseda, M.D. Pujol, C.A. Damonte, E.B. |
| author_sort |
Talarico, L.B. |
| title |
Anti-herpes simplex virus activity of sulfated galactans from the red seaweeds Gymnogongrus griffithsiae and Cryptonemia crenulata |
| title_short |
Anti-herpes simplex virus activity of sulfated galactans from the red seaweeds Gymnogongrus griffithsiae and Cryptonemia crenulata |
| title_full |
Anti-herpes simplex virus activity of sulfated galactans from the red seaweeds Gymnogongrus griffithsiae and Cryptonemia crenulata |
| title_fullStr |
Anti-herpes simplex virus activity of sulfated galactans from the red seaweeds Gymnogongrus griffithsiae and Cryptonemia crenulata |
| title_full_unstemmed |
Anti-herpes simplex virus activity of sulfated galactans from the red seaweeds Gymnogongrus griffithsiae and Cryptonemia crenulata |
| title_sort |
anti-herpes simplex virus activity of sulfated galactans from the red seaweeds gymnogongrus griffithsiae and cryptonemia crenulata |
| url |
http://hdl.handle.net/20.500.12110/paper_01418130_v34_n1-2_p63_Talarico |
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