Studies on the skeletal cell wall of the cystocarpic stage of the red seaweed Iridaea undulosa B.Part II. Fractionation of the cell wall and methylation analysis of the inner core-fibrillar polysaccharides
In order to determine the structure of the fibrillar cell wall, the material isolated from cystocarpic thalli of the red seaweed Iridaea undulosa was fractionated using different media. While classical methods produced a scarce solubilization of material, the use of lithium salts in polar aprotic so...
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_01418130_v27_n1_p21_Flores |
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todo:paper_01418130_v27_n1_p21_Flores2023-10-03T14:58:45Z Studies on the skeletal cell wall of the cystocarpic stage of the red seaweed Iridaea undulosa B.Part II. Fractionation of the cell wall and methylation analysis of the inner core-fibrillar polysaccharides Flores, M.L. Stortz, C.A. Cerezo, A.S. Cell wall Fibrillar polysaccharides Iridaea undulosa Rhodophyta cellulose dimethyl sulfoxide galactan glycoprotein lithium salt mannan n,n dimethylacetamide polysaccharide protein proteinase reagent solvent analytic method article cell wall chemical analysis controlled study extraction fractionation iridaea undulosa methylation nonhuman seaweed solubilization Acetylglucosamine Cell Extracts Cell Fractionation Cell Wall Methylation Monosaccharides Plant Proteins Polysaccharides Seaweed In order to determine the structure of the fibrillar cell wall, the material isolated from cystocarpic thalli of the red seaweed Iridaea undulosa was fractionated using different media. While classical methods produced a scarce solubilization of material, the use of lithium salts in polar aprotic solvents (dimethylsulfoxide or N,N-dimethylacetamide), had successfully extracted higher amounts of material. The final residue from the Li+/DMSO extraction contains cellulose and a mannan, while that from the Li+/DMAc extraction contains only cellulose and traces of a galactan. Methylation analyses of both residues confirm the presence of those polysaccharides, and shows that the mannan is (1→4)-linked. Treatment with proteases suggests that the protein is efficiently shielded from digestion. All the extracts and residues contain major amounts of (glyco)proteins and/or proteins, in agreement with a previous suggestion that they are of major importance in the structure of the cell wall. Copyright (C) 2000 Elsevier Science B.V. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01418130_v27_n1_p21_Flores |
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Universidad de Buenos Aires |
| institution_str |
I-28 |
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R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Cell wall Fibrillar polysaccharides Iridaea undulosa Rhodophyta cellulose dimethyl sulfoxide galactan glycoprotein lithium salt mannan n,n dimethylacetamide polysaccharide protein proteinase reagent solvent analytic method article cell wall chemical analysis controlled study extraction fractionation iridaea undulosa methylation nonhuman seaweed solubilization Acetylglucosamine Cell Extracts Cell Fractionation Cell Wall Methylation Monosaccharides Plant Proteins Polysaccharides Seaweed |
| spellingShingle |
Cell wall Fibrillar polysaccharides Iridaea undulosa Rhodophyta cellulose dimethyl sulfoxide galactan glycoprotein lithium salt mannan n,n dimethylacetamide polysaccharide protein proteinase reagent solvent analytic method article cell wall chemical analysis controlled study extraction fractionation iridaea undulosa methylation nonhuman seaweed solubilization Acetylglucosamine Cell Extracts Cell Fractionation Cell Wall Methylation Monosaccharides Plant Proteins Polysaccharides Seaweed Flores, M.L. Stortz, C.A. Cerezo, A.S. Studies on the skeletal cell wall of the cystocarpic stage of the red seaweed Iridaea undulosa B.Part II. Fractionation of the cell wall and methylation analysis of the inner core-fibrillar polysaccharides |
| topic_facet |
Cell wall Fibrillar polysaccharides Iridaea undulosa Rhodophyta cellulose dimethyl sulfoxide galactan glycoprotein lithium salt mannan n,n dimethylacetamide polysaccharide protein proteinase reagent solvent analytic method article cell wall chemical analysis controlled study extraction fractionation iridaea undulosa methylation nonhuman seaweed solubilization Acetylglucosamine Cell Extracts Cell Fractionation Cell Wall Methylation Monosaccharides Plant Proteins Polysaccharides Seaweed |
| description |
In order to determine the structure of the fibrillar cell wall, the material isolated from cystocarpic thalli of the red seaweed Iridaea undulosa was fractionated using different media. While classical methods produced a scarce solubilization of material, the use of lithium salts in polar aprotic solvents (dimethylsulfoxide or N,N-dimethylacetamide), had successfully extracted higher amounts of material. The final residue from the Li+/DMSO extraction contains cellulose and a mannan, while that from the Li+/DMAc extraction contains only cellulose and traces of a galactan. Methylation analyses of both residues confirm the presence of those polysaccharides, and shows that the mannan is (1→4)-linked. Treatment with proteases suggests that the protein is efficiently shielded from digestion. All the extracts and residues contain major amounts of (glyco)proteins and/or proteins, in agreement with a previous suggestion that they are of major importance in the structure of the cell wall. Copyright (C) 2000 Elsevier Science B.V. |
| format |
JOUR |
| author |
Flores, M.L. Stortz, C.A. Cerezo, A.S. |
| author_facet |
Flores, M.L. Stortz, C.A. Cerezo, A.S. |
| author_sort |
Flores, M.L. |
| title |
Studies on the skeletal cell wall of the cystocarpic stage of the red seaweed Iridaea undulosa B.Part II. Fractionation of the cell wall and methylation analysis of the inner core-fibrillar polysaccharides |
| title_short |
Studies on the skeletal cell wall of the cystocarpic stage of the red seaweed Iridaea undulosa B.Part II. Fractionation of the cell wall and methylation analysis of the inner core-fibrillar polysaccharides |
| title_full |
Studies on the skeletal cell wall of the cystocarpic stage of the red seaweed Iridaea undulosa B.Part II. Fractionation of the cell wall and methylation analysis of the inner core-fibrillar polysaccharides |
| title_fullStr |
Studies on the skeletal cell wall of the cystocarpic stage of the red seaweed Iridaea undulosa B.Part II. Fractionation of the cell wall and methylation analysis of the inner core-fibrillar polysaccharides |
| title_full_unstemmed |
Studies on the skeletal cell wall of the cystocarpic stage of the red seaweed Iridaea undulosa B.Part II. Fractionation of the cell wall and methylation analysis of the inner core-fibrillar polysaccharides |
| title_sort |
studies on the skeletal cell wall of the cystocarpic stage of the red seaweed iridaea undulosa b.part ii. fractionation of the cell wall and methylation analysis of the inner core-fibrillar polysaccharides |
| url |
http://hdl.handle.net/20.500.12110/paper_01418130_v27_n1_p21_Flores |
| work_keys_str_mv |
AT floresml studiesontheskeletalcellwallofthecystocarpicstageoftheredseaweediridaeaundulosabpartiifractionationofthecellwallandmethylationanalysisoftheinnercorefibrillarpolysaccharides AT stortzca studiesontheskeletalcellwallofthecystocarpicstageoftheredseaweediridaeaundulosabpartiifractionationofthecellwallandmethylationanalysisoftheinnercorefibrillarpolysaccharides AT cerezoas studiesontheskeletalcellwallofthecystocarpicstageoftheredseaweediridaeaundulosabpartiifractionationofthecellwallandmethylationanalysisoftheinnercorefibrillarpolysaccharides |
| _version_ |
1807317974468526080 |