Screening of catalytically active microorganisms for the synthesis of 6-modified purine nucleosides

Modified nucleosides can be prepared by microbial transglycosylation from cheaper nucleoside precursors using free or immobilised whole cells. An efficient screening method to find transglycosylation activity in microorganisms was developed for the synthesis of 6-modified purine nucleosides, such as...

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Detalles Bibliográficos
Autores principales:	Trelles, J.A., Valino, A.L., Runza, V., Lewkowicz, E.S., Iribarren, A.M.
Formato:	JOUR
Materias:	Bacillus stearothermophilus Microbial transglycosylation Nucleoside phosphorylases Nucleosides Screening Biosynthesis Catalysis Cells Enzymes Bacillus stearothermophiles Microorganisms nucleoside purine nucleoside purine nucleoside phosphorylase article bacterium biosynthesis catalysis economics enzymology Geobacillus stearothermophilus glycosylation isolation and purification metabolism methodology microbiology Bacteria Glycosylation Industrial Microbiology Purine Nucleosides Purine-Nucleoside Phosphorylase
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_01415492_v27_n11_p759_Trelles
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_01415492_v27_n11_p759_Trelles
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spelling	todo:paper_01415492_v27_n11_p759_Trelles2023-10-03T14:58:40Z Screening of catalytically active microorganisms for the synthesis of 6-modified purine nucleosides Trelles, J.A. Valino, A.L. Runza, V. Lewkowicz, E.S. Iribarren, A.M. Bacillus stearothermophilus Microbial transglycosylation Nucleoside phosphorylases Nucleosides Screening Biosynthesis Catalysis Cells Enzymes Screening Bacillus stearothermophiles Microbial transglycosylation Nucleoside phosphorylases Nucleosides Microorganisms nucleoside purine nucleoside purine nucleoside phosphorylase article bacterium biosynthesis catalysis economics enzymology Geobacillus stearothermophilus glycosylation isolation and purification metabolism methodology microbiology Bacillus stearothermophilus Bacteria Catalysis Glycosylation Industrial Microbiology Nucleosides Purine Nucleosides Purine-Nucleoside Phosphorylase Geobacillus stearothermophilus Modified nucleosides can be prepared by microbial transglycosylation from cheaper nucleoside precursors using free or immobilised whole cells. An efficient screening method to find transglycosylation activity in microorganisms was developed for the synthesis of 6-modified purine nucleosides, such as 6-chloro-, 6-methoxy-, 6-iodo- and 6-mercaptopurine ribonucleoside. Out of 100 microorganisms screened, Bacillus stearothermophilus ATCC 12980 was the best for this purpose. © Springer 2005. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01415492_v27_n11_p759_Trelles
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Bacillus stearothermophilus Microbial transglycosylation Nucleoside phosphorylases Nucleosides Screening Biosynthesis Catalysis Cells Enzymes Screening Bacillus stearothermophiles Microbial transglycosylation Nucleoside phosphorylases Nucleosides Microorganisms nucleoside purine nucleoside purine nucleoside phosphorylase article bacterium biosynthesis catalysis economics enzymology Geobacillus stearothermophilus glycosylation isolation and purification metabolism methodology microbiology Bacillus stearothermophilus Bacteria Catalysis Glycosylation Industrial Microbiology Nucleosides Purine Nucleosides Purine-Nucleoside Phosphorylase Geobacillus stearothermophilus
spellingShingle	Bacillus stearothermophilus Microbial transglycosylation Nucleoside phosphorylases Nucleosides Screening Biosynthesis Catalysis Cells Enzymes Screening Bacillus stearothermophiles Microbial transglycosylation Nucleoside phosphorylases Nucleosides Microorganisms nucleoside purine nucleoside purine nucleoside phosphorylase article bacterium biosynthesis catalysis economics enzymology Geobacillus stearothermophilus glycosylation isolation and purification metabolism methodology microbiology Bacillus stearothermophilus Bacteria Catalysis Glycosylation Industrial Microbiology Nucleosides Purine Nucleosides Purine-Nucleoside Phosphorylase Geobacillus stearothermophilus Trelles, J.A. Valino, A.L. Runza, V. Lewkowicz, E.S. Iribarren, A.M. Screening of catalytically active microorganisms for the synthesis of 6-modified purine nucleosides
topic_facet	Bacillus stearothermophilus Microbial transglycosylation Nucleoside phosphorylases Nucleosides Screening Biosynthesis Catalysis Cells Enzymes Screening Bacillus stearothermophiles Microbial transglycosylation Nucleoside phosphorylases Nucleosides Microorganisms nucleoside purine nucleoside purine nucleoside phosphorylase article bacterium biosynthesis catalysis economics enzymology Geobacillus stearothermophilus glycosylation isolation and purification metabolism methodology microbiology Bacillus stearothermophilus Bacteria Catalysis Glycosylation Industrial Microbiology Nucleosides Purine Nucleosides Purine-Nucleoside Phosphorylase Geobacillus stearothermophilus
description	Modified nucleosides can be prepared by microbial transglycosylation from cheaper nucleoside precursors using free or immobilised whole cells. An efficient screening method to find transglycosylation activity in microorganisms was developed for the synthesis of 6-modified purine nucleosides, such as 6-chloro-, 6-methoxy-, 6-iodo- and 6-mercaptopurine ribonucleoside. Out of 100 microorganisms screened, Bacillus stearothermophilus ATCC 12980 was the best for this purpose. © Springer 2005.
format	JOUR
author	Trelles, J.A. Valino, A.L. Runza, V. Lewkowicz, E.S. Iribarren, A.M.
author_facet	Trelles, J.A. Valino, A.L. Runza, V. Lewkowicz, E.S. Iribarren, A.M.
author_sort	Trelles, J.A.
title	Screening of catalytically active microorganisms for the synthesis of 6-modified purine nucleosides
title_short	Screening of catalytically active microorganisms for the synthesis of 6-modified purine nucleosides
title_full	Screening of catalytically active microorganisms for the synthesis of 6-modified purine nucleosides
title_fullStr	Screening of catalytically active microorganisms for the synthesis of 6-modified purine nucleosides
title_full_unstemmed	Screening of catalytically active microorganisms for the synthesis of 6-modified purine nucleosides
title_sort	screening of catalytically active microorganisms for the synthesis of 6-modified purine nucleosides
url	http://hdl.handle.net/20.500.12110/paper_01415492_v27_n11_p759_Trelles
work_keys_str_mv	AT trellesja screeningofcatalyticallyactivemicroorganismsforthesynthesisof6modifiedpurinenucleosides AT valinoal screeningofcatalyticallyactivemicroorganismsforthesynthesisof6modifiedpurinenucleosides AT runzav screeningofcatalyticallyactivemicroorganismsforthesynthesisof6modifiedpurinenucleosides AT lewkowiczes screeningofcatalyticallyactivemicroorganismsforthesynthesisof6modifiedpurinenucleosides AT iribarrenam screeningofcatalyticallyactivemicroorganismsforthesynthesisof6modifiedpurinenucleosides
_version_	1807322878444568576

Screening of catalytically active microorganisms for the synthesis of 6-modified purine nucleosides

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