A simple and efficient enzymatic procedure for the deprotection of two base labile chlorinated purine ribosides
Base-labile 6-chloro-2′,3′,5′-tri-O-acetylpurine riboside (1c) and 2-amino-6-chloro-2′,3′,5′-tri-O-acetylpurine riboside (1d) were fully deacetylated through Candida antarctica B lipase hydrolysis, affording respectively 6-chloropurine riboside (2c) and 2-amino-6-chloro-purine riboside (2d). Quantit...
Guardado en:
| Autores principales: | , , , |
|---|---|
| Formato: | JOUR |
| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_01415492_v23_n17_p1439_Roncaglia |
| Aporte de: |
| id |
todo:paper_01415492_v23_n17_p1439_Roncaglia |
|---|---|
| record_format |
dspace |
| spelling |
todo:paper_01415492_v23_n17_p1439_Roncaglia2023-10-03T14:58:39Z A simple and efficient enzymatic procedure for the deprotection of two base labile chlorinated purine ribosides Roncaglia, D.I. Schmidt, A.M. Iglesias, L.E. Iribarren, A.M. 2-amino-6-chloropurine riboside 6-chloropurine riboside Deacetylation Lipases Nucleosides 2 amino 6 chloropurine riboside 6 chloropurine ribonucleoside nebularine nucleoside purine derivative purine nucleoside triacylglycerol lipase unclassified drug article Aspergillus Burkholderia cepacia Candida catalysis enzyme activity hydrolysis Mucor nonhuman thin layer chromatography Base-labile 6-chloro-2′,3′,5′-tri-O-acetylpurine riboside (1c) and 2-amino-6-chloro-2′,3′,5′-tri-O-acetylpurine riboside (1d) were fully deacetylated through Candida antarctica B lipase hydrolysis, affording respectively 6-chloropurine riboside (2c) and 2-amino-6-chloro-purine riboside (2d). Quantitative results were found at pH 7 and 60 °C in 24 h for 1c and 72 h for 1d. This mild and simple enzymatic technique represents a convenient procedure for the removal of acetyl groups from such base labile halogenated nucleosides. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01415492_v23_n17_p1439_Roncaglia |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
2-amino-6-chloropurine riboside 6-chloropurine riboside Deacetylation Lipases Nucleosides 2 amino 6 chloropurine riboside 6 chloropurine ribonucleoside nebularine nucleoside purine derivative purine nucleoside triacylglycerol lipase unclassified drug article Aspergillus Burkholderia cepacia Candida catalysis enzyme activity hydrolysis Mucor nonhuman thin layer chromatography |
| spellingShingle |
2-amino-6-chloropurine riboside 6-chloropurine riboside Deacetylation Lipases Nucleosides 2 amino 6 chloropurine riboside 6 chloropurine ribonucleoside nebularine nucleoside purine derivative purine nucleoside triacylglycerol lipase unclassified drug article Aspergillus Burkholderia cepacia Candida catalysis enzyme activity hydrolysis Mucor nonhuman thin layer chromatography Roncaglia, D.I. Schmidt, A.M. Iglesias, L.E. Iribarren, A.M. A simple and efficient enzymatic procedure for the deprotection of two base labile chlorinated purine ribosides |
| topic_facet |
2-amino-6-chloropurine riboside 6-chloropurine riboside Deacetylation Lipases Nucleosides 2 amino 6 chloropurine riboside 6 chloropurine ribonucleoside nebularine nucleoside purine derivative purine nucleoside triacylglycerol lipase unclassified drug article Aspergillus Burkholderia cepacia Candida catalysis enzyme activity hydrolysis Mucor nonhuman thin layer chromatography |
| description |
Base-labile 6-chloro-2′,3′,5′-tri-O-acetylpurine riboside (1c) and 2-amino-6-chloro-2′,3′,5′-tri-O-acetylpurine riboside (1d) were fully deacetylated through Candida antarctica B lipase hydrolysis, affording respectively 6-chloropurine riboside (2c) and 2-amino-6-chloro-purine riboside (2d). Quantitative results were found at pH 7 and 60 °C in 24 h for 1c and 72 h for 1d. This mild and simple enzymatic technique represents a convenient procedure for the removal of acetyl groups from such base labile halogenated nucleosides. |
| format |
JOUR |
| author |
Roncaglia, D.I. Schmidt, A.M. Iglesias, L.E. Iribarren, A.M. |
| author_facet |
Roncaglia, D.I. Schmidt, A.M. Iglesias, L.E. Iribarren, A.M. |
| author_sort |
Roncaglia, D.I. |
| title |
A simple and efficient enzymatic procedure for the deprotection of two base labile chlorinated purine ribosides |
| title_short |
A simple and efficient enzymatic procedure for the deprotection of two base labile chlorinated purine ribosides |
| title_full |
A simple and efficient enzymatic procedure for the deprotection of two base labile chlorinated purine ribosides |
| title_fullStr |
A simple and efficient enzymatic procedure for the deprotection of two base labile chlorinated purine ribosides |
| title_full_unstemmed |
A simple and efficient enzymatic procedure for the deprotection of two base labile chlorinated purine ribosides |
| title_sort |
simple and efficient enzymatic procedure for the deprotection of two base labile chlorinated purine ribosides |
| url |
http://hdl.handle.net/20.500.12110/paper_01415492_v23_n17_p1439_Roncaglia |
| work_keys_str_mv |
AT roncagliadi asimpleandefficientenzymaticprocedureforthedeprotectionoftwobaselabilechlorinatedpurineribosides AT schmidtam asimpleandefficientenzymaticprocedureforthedeprotectionoftwobaselabilechlorinatedpurineribosides AT iglesiasle asimpleandefficientenzymaticprocedureforthedeprotectionoftwobaselabilechlorinatedpurineribosides AT iribarrenam asimpleandefficientenzymaticprocedureforthedeprotectionoftwobaselabilechlorinatedpurineribosides AT roncagliadi simpleandefficientenzymaticprocedureforthedeprotectionoftwobaselabilechlorinatedpurineribosides AT schmidtam simpleandefficientenzymaticprocedureforthedeprotectionoftwobaselabilechlorinatedpurineribosides AT iglesiasle simpleandefficientenzymaticprocedureforthedeprotectionoftwobaselabilechlorinatedpurineribosides AT iribarrenam simpleandefficientenzymaticprocedureforthedeprotectionoftwobaselabilechlorinatedpurineribosides |
| _version_ |
1782029686820306944 |