Chemo- and stereoselective reduction of β-keto esters by spores and various morphological forms of Mucor rouxii
We report the efficient enantioselective reduction of a number of β-ketoesters by mycelial and yeast-like forms of the dimorphic fungus Mucor rouxii in a whole-cell process. Mycelial cells, grown in aerobiosis, were efficient in water, whereas the yeast-like cells, grown in anaerobic medium, were bo...
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todo:paper_01410229_v30_n5_p596_Mangone2023-10-03T14:58:32Z Chemo- and stereoselective reduction of β-keto esters by spores and various morphological forms of Mucor rouxii Mangone, C.P. N.Pereyra, E. Argimón, S. Moreno, S. Baldessari, A. β-Ketoesters Mucor rouxii Mycelial Spores Yeast Biomass Cells Esters Fungi Organic solvents Water Yeast Aerobiosis Stereoselectivity Reduction ester derivative organic solvent water aerobic metabolism article bacterial spore biomass catalysis controlled study enantiomer enantioselectivity fungal cell fungus fungus growth fungus spore morphology Mucor mucor rouxii mycelium nonhuman physiology reduction rehydration stereochemistry yeast Amylomyces rouxii Fungi Mucor We report the efficient enantioselective reduction of a number of β-ketoesters by mycelial and yeast-like forms of the dimorphic fungus Mucor rouxii in a whole-cell process. Mycelial cells, grown in aerobiosis, were efficient in water, whereas the yeast-like cells, grown in anaerobic medium, were both efficient in water and in organic solvents. Almost 100% of conversion with 97% of enantiomeric excess of the (S) forms of the reduced β-ketoesters was obtained at 15 g biomass (wet weight)/mmol substrate in two hours. The fungal spores, which are the physiologically resistant form of the fungus, also catalyzed the reductive process efficiently and stereoselectively. The freeze-dried as well as the warm-air dried yeast-like cells, rehydrated in a small volume of water, maintained the same efficiency and selectivity of the reaction in organic solvents as the fresh biomass up to at least 4 months. © 2002 Elsevier Science Inc. All rights reserved. Fil:Mangone, C.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:N.Pereyra, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Argimón, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Moreno, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Baldessari, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01410229_v30_n5_p596_Mangone |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
β-Ketoesters Mucor rouxii Mycelial Spores Yeast Biomass Cells Esters Fungi Organic solvents Water Yeast Aerobiosis Stereoselectivity Reduction ester derivative organic solvent water aerobic metabolism article bacterial spore biomass catalysis controlled study enantiomer enantioselectivity fungal cell fungus fungus growth fungus spore morphology Mucor mucor rouxii mycelium nonhuman physiology reduction rehydration stereochemistry yeast Amylomyces rouxii Fungi Mucor |
| spellingShingle |
β-Ketoesters Mucor rouxii Mycelial Spores Yeast Biomass Cells Esters Fungi Organic solvents Water Yeast Aerobiosis Stereoselectivity Reduction ester derivative organic solvent water aerobic metabolism article bacterial spore biomass catalysis controlled study enantiomer enantioselectivity fungal cell fungus fungus growth fungus spore morphology Mucor mucor rouxii mycelium nonhuman physiology reduction rehydration stereochemistry yeast Amylomyces rouxii Fungi Mucor Mangone, C.P. N.Pereyra, E. Argimón, S. Moreno, S. Baldessari, A. Chemo- and stereoselective reduction of β-keto esters by spores and various morphological forms of Mucor rouxii |
| topic_facet |
β-Ketoesters Mucor rouxii Mycelial Spores Yeast Biomass Cells Esters Fungi Organic solvents Water Yeast Aerobiosis Stereoselectivity Reduction ester derivative organic solvent water aerobic metabolism article bacterial spore biomass catalysis controlled study enantiomer enantioselectivity fungal cell fungus fungus growth fungus spore morphology Mucor mucor rouxii mycelium nonhuman physiology reduction rehydration stereochemistry yeast Amylomyces rouxii Fungi Mucor |
| description |
We report the efficient enantioselective reduction of a number of β-ketoesters by mycelial and yeast-like forms of the dimorphic fungus Mucor rouxii in a whole-cell process. Mycelial cells, grown in aerobiosis, were efficient in water, whereas the yeast-like cells, grown in anaerobic medium, were both efficient in water and in organic solvents. Almost 100% of conversion with 97% of enantiomeric excess of the (S) forms of the reduced β-ketoesters was obtained at 15 g biomass (wet weight)/mmol substrate in two hours. The fungal spores, which are the physiologically resistant form of the fungus, also catalyzed the reductive process efficiently and stereoselectively. The freeze-dried as well as the warm-air dried yeast-like cells, rehydrated in a small volume of water, maintained the same efficiency and selectivity of the reaction in organic solvents as the fresh biomass up to at least 4 months. © 2002 Elsevier Science Inc. All rights reserved. |
| format |
JOUR |
| author |
Mangone, C.P. N.Pereyra, E. Argimón, S. Moreno, S. Baldessari, A. |
| author_facet |
Mangone, C.P. N.Pereyra, E. Argimón, S. Moreno, S. Baldessari, A. |
| author_sort |
Mangone, C.P. |
| title |
Chemo- and stereoselective reduction of β-keto esters by spores and various morphological forms of Mucor rouxii |
| title_short |
Chemo- and stereoselective reduction of β-keto esters by spores and various morphological forms of Mucor rouxii |
| title_full |
Chemo- and stereoselective reduction of β-keto esters by spores and various morphological forms of Mucor rouxii |
| title_fullStr |
Chemo- and stereoselective reduction of β-keto esters by spores and various morphological forms of Mucor rouxii |
| title_full_unstemmed |
Chemo- and stereoselective reduction of β-keto esters by spores and various morphological forms of Mucor rouxii |
| title_sort |
chemo- and stereoselective reduction of β-keto esters by spores and various morphological forms of mucor rouxii |
| url |
http://hdl.handle.net/20.500.12110/paper_01410229_v30_n5_p596_Mangone |
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