Reduced expression of selected FASCICLIN-LIKE ARABINOGALACTAN PROTEIN genes associates with the abortion of kernels in field crops of Zea mays (maize) and of Arabidopsis seeds

Abortion of fertilized ovaries at the tip of the ear can generate significant yield losses in maize crops. To investigate the mechanisms involved in this process, 2 maize hybrids were grown in field crops at 2 sowing densities and under 3 irrigation regimes (well-watered control, drought before poll...

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Autores principales:	Cagnola, J.I., Dumont de Chassart, G.J., Ibarra, S.E., Chimenti, C., Ricardi, M.M., Delzer, B., Ghiglione, H., Zhu, T., Otegui, M.E., Estevez, J.M., Casal, J.J.
Formato:	JOUR
Materias:	corn drought stress FLA seed abortion gene expression irrigation maize mutation protein seed sowing sugar Arabidopsis Arabidopsis thaliana Zea mays Arabidopsis protein arabinogalactan proteins glucoside mucoprotein phloroglucinol plant protein Yariv reagent analogs and derivatives chimera drought drug effect gene expression regulation genetics metabolism ovule physiology plant seed pollination transgenic plant Arabidopsis Proteins Chimera Droughts Gene Expression Regulation, Plant Glucosides Mucoproteins Ovule Phloroglucinol Plant Proteins Plants, Genetically Modified Pollination Seeds
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_01407791_v41_n3_p661_Cagnola
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	todo:paper_01407791_v41_n3_p661_Cagnola2023-10-03T14:58:30Z Reduced expression of selected FASCICLIN-LIKE ARABINOGALACTAN PROTEIN genes associates with the abortion of kernels in field crops of Zea mays (maize) and of Arabidopsis seeds Cagnola, J.I. Dumont de Chassart, G.J. Ibarra, S.E. Chimenti, C. Ricardi, M.M. Delzer, B. Ghiglione, H. Zhu, T. Otegui, M.E. Estevez, J.M. Casal, J.J. corn drought stress FLA seed abortion drought stress gene expression irrigation maize mutation protein seed sowing sugar Arabidopsis Arabidopsis thaliana Zea mays Arabidopsis protein arabinogalactan proteins glucoside mucoprotein phloroglucinol plant protein Yariv reagent analogs and derivatives Arabidopsis chimera drought drug effect gene expression regulation genetics maize metabolism ovule physiology plant seed pollination transgenic plant Arabidopsis Arabidopsis Proteins Chimera Droughts Gene Expression Regulation, Plant Glucosides Mucoproteins Ovule Phloroglucinol Plant Proteins Plants, Genetically Modified Pollination Seeds Zea mays Abortion of fertilized ovaries at the tip of the ear can generate significant yield losses in maize crops. To investigate the mechanisms involved in this process, 2 maize hybrids were grown in field crops at 2 sowing densities and under 3 irrigation regimes (well-watered control, drought before pollination, and drought during pollination), in all possible combinations. Samples of ear tips were taken 2–6 days after synchronous hand pollination and used for the analysis of gene expression and sugars. Glucose and fructose levels increased in kernels with high abortion risk. Several FASCICLIN-LIKE ARABINOGALACTAN PROTEIN (FLA) genes showed negative correlation with abortion. The expression of ZmFLA7 responded to drought only at the tip of the ear. The abundance of arabinogalactan protein (AGP) glycan epitopes decreased with drought and pharmacological treatments that reduce AGP activity enhanced the abortion of fertilized ovaries. Drought also reduced the expression of AthFLA9 in the siliques of Arabidopsis thaliana. Gain- and loss-of-function mutants of Arabidopsis showed a negative correlation between AthFLA9 and seed abortion. On the basis of gene expression patterns, pharmacological, and genetic evidence, we propose that stress-induced reductions in the expression of selected FLA genes enhance abortion of fertilized ovaries in maize and Arabidopsis. © 2018 John Wiley & Sons Ltd JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01407791_v41_n3_p661_Cagnola
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	corn drought stress FLA seed abortion drought stress gene expression irrigation maize mutation protein seed sowing sugar Arabidopsis Arabidopsis thaliana Zea mays Arabidopsis protein arabinogalactan proteins glucoside mucoprotein phloroglucinol plant protein Yariv reagent analogs and derivatives Arabidopsis chimera drought drug effect gene expression regulation genetics maize metabolism ovule physiology plant seed pollination transgenic plant Arabidopsis Arabidopsis Proteins Chimera Droughts Gene Expression Regulation, Plant Glucosides Mucoproteins Ovule Phloroglucinol Plant Proteins Plants, Genetically Modified Pollination Seeds Zea mays
spellingShingle	corn drought stress FLA seed abortion drought stress gene expression irrigation maize mutation protein seed sowing sugar Arabidopsis Arabidopsis thaliana Zea mays Arabidopsis protein arabinogalactan proteins glucoside mucoprotein phloroglucinol plant protein Yariv reagent analogs and derivatives Arabidopsis chimera drought drug effect gene expression regulation genetics maize metabolism ovule physiology plant seed pollination transgenic plant Arabidopsis Arabidopsis Proteins Chimera Droughts Gene Expression Regulation, Plant Glucosides Mucoproteins Ovule Phloroglucinol Plant Proteins Plants, Genetically Modified Pollination Seeds Zea mays Cagnola, J.I. Dumont de Chassart, G.J. Ibarra, S.E. Chimenti, C. Ricardi, M.M. Delzer, B. Ghiglione, H. Zhu, T. Otegui, M.E. Estevez, J.M. Casal, J.J. Reduced expression of selected FASCICLIN-LIKE ARABINOGALACTAN PROTEIN genes associates with the abortion of kernels in field crops of Zea mays (maize) and of Arabidopsis seeds
topic_facet	corn drought stress FLA seed abortion drought stress gene expression irrigation maize mutation protein seed sowing sugar Arabidopsis Arabidopsis thaliana Zea mays Arabidopsis protein arabinogalactan proteins glucoside mucoprotein phloroglucinol plant protein Yariv reagent analogs and derivatives Arabidopsis chimera drought drug effect gene expression regulation genetics maize metabolism ovule physiology plant seed pollination transgenic plant Arabidopsis Arabidopsis Proteins Chimera Droughts Gene Expression Regulation, Plant Glucosides Mucoproteins Ovule Phloroglucinol Plant Proteins Plants, Genetically Modified Pollination Seeds Zea mays
description	Abortion of fertilized ovaries at the tip of the ear can generate significant yield losses in maize crops. To investigate the mechanisms involved in this process, 2 maize hybrids were grown in field crops at 2 sowing densities and under 3 irrigation regimes (well-watered control, drought before pollination, and drought during pollination), in all possible combinations. Samples of ear tips were taken 2–6 days after synchronous hand pollination and used for the analysis of gene expression and sugars. Glucose and fructose levels increased in kernels with high abortion risk. Several FASCICLIN-LIKE ARABINOGALACTAN PROTEIN (FLA) genes showed negative correlation with abortion. The expression of ZmFLA7 responded to drought only at the tip of the ear. The abundance of arabinogalactan protein (AGP) glycan epitopes decreased with drought and pharmacological treatments that reduce AGP activity enhanced the abortion of fertilized ovaries. Drought also reduced the expression of AthFLA9 in the siliques of Arabidopsis thaliana. Gain- and loss-of-function mutants of Arabidopsis showed a negative correlation between AthFLA9 and seed abortion. On the basis of gene expression patterns, pharmacological, and genetic evidence, we propose that stress-induced reductions in the expression of selected FLA genes enhance abortion of fertilized ovaries in maize and Arabidopsis. © 2018 John Wiley & Sons Ltd
format	JOUR
author	Cagnola, J.I. Dumont de Chassart, G.J. Ibarra, S.E. Chimenti, C. Ricardi, M.M. Delzer, B. Ghiglione, H. Zhu, T. Otegui, M.E. Estevez, J.M. Casal, J.J.
author_facet	Cagnola, J.I. Dumont de Chassart, G.J. Ibarra, S.E. Chimenti, C. Ricardi, M.M. Delzer, B. Ghiglione, H. Zhu, T. Otegui, M.E. Estevez, J.M. Casal, J.J.
author_sort	Cagnola, J.I.
title	Reduced expression of selected FASCICLIN-LIKE ARABINOGALACTAN PROTEIN genes associates with the abortion of kernels in field crops of Zea mays (maize) and of Arabidopsis seeds
title_short	Reduced expression of selected FASCICLIN-LIKE ARABINOGALACTAN PROTEIN genes associates with the abortion of kernels in field crops of Zea mays (maize) and of Arabidopsis seeds
title_full	Reduced expression of selected FASCICLIN-LIKE ARABINOGALACTAN PROTEIN genes associates with the abortion of kernels in field crops of Zea mays (maize) and of Arabidopsis seeds
title_fullStr	Reduced expression of selected FASCICLIN-LIKE ARABINOGALACTAN PROTEIN genes associates with the abortion of kernels in field crops of Zea mays (maize) and of Arabidopsis seeds
title_full_unstemmed	Reduced expression of selected FASCICLIN-LIKE ARABINOGALACTAN PROTEIN genes associates with the abortion of kernels in field crops of Zea mays (maize) and of Arabidopsis seeds
title_sort	reduced expression of selected fasciclin-like arabinogalactan protein genes associates with the abortion of kernels in field crops of zea mays (maize) and of arabidopsis seeds
url	http://hdl.handle.net/20.500.12110/paper_01407791_v41_n3_p661_Cagnola
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Reduced expression of selected FASCICLIN-LIKE ARABINOGALACTAN PROTEIN genes associates with the abortion of kernels in field crops of Zea mays (maize) and of Arabidopsis seeds

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