Comparison of two coprological methods for the veterinary diagnosis of fasciolosis

The sensitivity and utility of a standard faecal sedimentation method (FSM) and a modified stool sieving staining method (FSSM), both currently employed for the diagnosis of Fasciola hepatica infection were compared. Faecal samples were obtained from 51 bovines of an endemic area for fasciolosis in...

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Autores principales:	Kleiman, F., Pietrokovsky, S., Gil, S., Wisnivesky-Colli, C.
Formato:	JOUR
Materias:	Cattle Coprological method Diagnosis Fasciola hepatica Bos taurus
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_01020935_v57_n2_p181_Kleiman
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_01020935_v57_n2_p181_Kleiman
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spelling	todo:paper_01020935_v57_n2_p181_Kleiman2023-10-03T14:57:23Z Comparison of two coprological methods for the veterinary diagnosis of fasciolosis Kleiman, F. Pietrokovsky, S. Gil, S. Wisnivesky-Colli, C. Cattle Coprological method Diagnosis Fasciola hepatica Bos taurus Fasciola hepatica The sensitivity and utility of a standard faecal sedimentation method (FSM) and a modified stool sieving staining method (FSSM), both currently employed for the diagnosis of Fasciola hepatica infection were compared. Faecal samples were obtained from 51 bovines of an endemic area for fasciolosis in Southwestern Argentina. Each sample was placed in a recipient containing 5% formalin. Eight millilitres of the suspension, equivalent to 2g of faeces, were used for each of the two methods tested. The number of eggs found per sample was recorded. The proportion of positive samples obtained by the FSSM (27/51) was significantly higher than that by the FSM (11/51) (P<0.05). The percent of agreement between methods was 41%. Over a total of 27 positive samples detected by the FSSM, the FSM missed 16, yielding 60% false negative samples. The FSSM enhanced 2.5 times the sensitivity of diagnosis. The complexity of the FSM may decrease its sensitivity through missing and loss of eggs during sample processing. These results confirmed that the commonly used FSM underestimates the prevalence and the egg output in cattle and that the FSSM is a more reliable diagnostic method. Fil:Kleiman, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pietrokovsky, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Wisnivesky-Colli, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01020935_v57_n2_p181_Kleiman
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Cattle Coprological method Diagnosis Fasciola hepatica Bos taurus Fasciola hepatica
spellingShingle	Cattle Coprological method Diagnosis Fasciola hepatica Bos taurus Fasciola hepatica Kleiman, F. Pietrokovsky, S. Gil, S. Wisnivesky-Colli, C. Comparison of two coprological methods for the veterinary diagnosis of fasciolosis
topic_facet	Cattle Coprological method Diagnosis Fasciola hepatica Bos taurus Fasciola hepatica
description	The sensitivity and utility of a standard faecal sedimentation method (FSM) and a modified stool sieving staining method (FSSM), both currently employed for the diagnosis of Fasciola hepatica infection were compared. Faecal samples were obtained from 51 bovines of an endemic area for fasciolosis in Southwestern Argentina. Each sample was placed in a recipient containing 5% formalin. Eight millilitres of the suspension, equivalent to 2g of faeces, were used for each of the two methods tested. The number of eggs found per sample was recorded. The proportion of positive samples obtained by the FSSM (27/51) was significantly higher than that by the FSM (11/51) (P<0.05). The percent of agreement between methods was 41%. Over a total of 27 positive samples detected by the FSSM, the FSM missed 16, yielding 60% false negative samples. The FSSM enhanced 2.5 times the sensitivity of diagnosis. The complexity of the FSM may decrease its sensitivity through missing and loss of eggs during sample processing. These results confirmed that the commonly used FSM underestimates the prevalence and the egg output in cattle and that the FSSM is a more reliable diagnostic method.
format	JOUR
author	Kleiman, F. Pietrokovsky, S. Gil, S. Wisnivesky-Colli, C.
author_facet	Kleiman, F. Pietrokovsky, S. Gil, S. Wisnivesky-Colli, C.
author_sort	Kleiman, F.
title	Comparison of two coprological methods for the veterinary diagnosis of fasciolosis
title_short	Comparison of two coprological methods for the veterinary diagnosis of fasciolosis
title_full	Comparison of two coprological methods for the veterinary diagnosis of fasciolosis
title_fullStr	Comparison of two coprological methods for the veterinary diagnosis of fasciolosis
title_full_unstemmed	Comparison of two coprological methods for the veterinary diagnosis of fasciolosis
title_sort	comparison of two coprological methods for the veterinary diagnosis of fasciolosis
url	http://hdl.handle.net/20.500.12110/paper_01020935_v57_n2_p181_Kleiman
work_keys_str_mv	AT kleimanf comparisonoftwocoprologicalmethodsfortheveterinarydiagnosisoffasciolosis AT pietrokovskys comparisonoftwocoprologicalmethodsfortheveterinarydiagnosisoffasciolosis AT gils comparisonoftwocoprologicalmethodsfortheveterinarydiagnosisoffasciolosis AT wisniveskycollic comparisonoftwocoprologicalmethodsfortheveterinarydiagnosisoffasciolosis
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Comparison of two coprological methods for the veterinary diagnosis of fasciolosis

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