A novel procedure to measure the antioxidant capacity of yerba maté extracts

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Yerba maté extracts have in vitro antioxidant capacity attributed to the presence of polyphenolic compounds, mainly chlorogenic acids and dicaffeoylquinic acid derivatives. DPPH is one of the most used assays to measure the antioxidant capacity of pure compounds and plant extracts. It is difficult t...

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Autores principales: Hartwig, V.G., Brumovsky, L.A., Fretes, R.M., Boado, L.S.
Formato: JOUR
Materias:
Antioxidant capacity
Dpph
Ilex paraguariensis
Yerba maté
Croton ovalifolius
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_01012061_v32_n1_p126_Hartwig
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_01012061_v32_n1_p126_Hartwig
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spelling todo:paper_01012061_v32_n1_p126_Hartwig2023-10-03T14:57:21Z A novel procedure to measure the antioxidant capacity of yerba maté extracts Hartwig, V.G. Brumovsky, L.A. Fretes, R.M. Boado, L.S. Antioxidant capacity Dpph Ilex paraguariensis Yerba maté Croton ovalifolius Ilex paraguariensis Yerba maté extracts have in vitro antioxidant capacity attributed to the presence of polyphenolic compounds, mainly chlorogenic acids and dicaffeoylquinic acid derivatives. DPPH is one of the most used assays to measure the antioxidant capacity of pure compounds and plant extracts. It is difficult to compare the results between studies because this assay is applied in too many different conditions by the different research groups. Thus, in order to assess the antioxidant capacity of yerba maté extracts, the following procedure is proposed: 100 μL of an aqueous dilution of the extracts is mixed in duplicate with 3.0 mL of a DPPH 'work solution in absolute methanol (100 μM.L-1), with an incubation time of 120 minutes in darkness at 37 ± 1 °C, and then absorbance is read at 517 nm against absolute methanol. The results should be expressed as ascorbic acid equivalents or Trolox equivalents in mass percentage (g% dm, dry matter) in order to facilitate comparisons. The AOC of the ethanolic extracts ranged between 12.8 and 23.1 g TE % dm and from 9.1 to 16.4 g AAE % dm. The AOC determined by the DPPH assay proposed in the present study can be related to the total polyphenolic content determined by the Folin-Ciocalteu assay. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01012061_v32_n1_p126_Hartwig
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Antioxidant capacity
Dpph
Ilex paraguariensis
Yerba maté
Croton ovalifolius
Ilex paraguariensis
spellingShingle Antioxidant capacity
Dpph
Ilex paraguariensis
Yerba maté
Croton ovalifolius
Ilex paraguariensis
Hartwig, V.G.
Brumovsky, L.A.
Fretes, R.M.
Boado, L.S.
A novel procedure to measure the antioxidant capacity of yerba maté extracts
topic_facet Antioxidant capacity
Dpph
Ilex paraguariensis
Yerba maté
Croton ovalifolius
Ilex paraguariensis
description Yerba maté extracts have in vitro antioxidant capacity attributed to the presence of polyphenolic compounds, mainly chlorogenic acids and dicaffeoylquinic acid derivatives. DPPH is one of the most used assays to measure the antioxidant capacity of pure compounds and plant extracts. It is difficult to compare the results between studies because this assay is applied in too many different conditions by the different research groups. Thus, in order to assess the antioxidant capacity of yerba maté extracts, the following procedure is proposed: 100 μL of an aqueous dilution of the extracts is mixed in duplicate with 3.0 mL of a DPPH 'work solution in absolute methanol (100 μM.L-1), with an incubation time of 120 minutes in darkness at 37 ± 1 °C, and then absorbance is read at 517 nm against absolute methanol. The results should be expressed as ascorbic acid equivalents or Trolox equivalents in mass percentage (g% dm, dry matter) in order to facilitate comparisons. The AOC of the ethanolic extracts ranged between 12.8 and 23.1 g TE % dm and from 9.1 to 16.4 g AAE % dm. The AOC determined by the DPPH assay proposed in the present study can be related to the total polyphenolic content determined by the Folin-Ciocalteu assay.
format JOUR
author Hartwig, V.G.
Brumovsky, L.A.
Fretes, R.M.
Boado, L.S.
author_facet Hartwig, V.G.
Brumovsky, L.A.
Fretes, R.M.
Boado, L.S.
author_sort Hartwig, V.G.
title A novel procedure to measure the antioxidant capacity of yerba maté extracts
title_short A novel procedure to measure the antioxidant capacity of yerba maté extracts
title_full A novel procedure to measure the antioxidant capacity of yerba maté extracts
title_fullStr A novel procedure to measure the antioxidant capacity of yerba maté extracts
title_full_unstemmed A novel procedure to measure the antioxidant capacity of yerba maté extracts
title_sort novel procedure to measure the antioxidant capacity of yerba maté extracts
url http://hdl.handle.net/20.500.12110/paper_01012061_v32_n1_p126_Hartwig
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