In vivo photorelease of GABA in the mouse cortex

Electrical stimulation has been used for more than 100 years in neuroscientific and biomedical research as a powerful tool for controlled perturbations of neural activity. Despite quickly driving neuronal activity, this technique presents some important limitations, such as the impossibility to acti...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Lopes-dos-Santos, V., Campi, J., Filevich, O., Ribeiro, S., Etchenique, R.
Formato:	JOUR
Materias:	Caged compounds Cerebral cortex GABA Neural stimulation Photorelease 4 aminobutyric acid 4 aminobutyric acid release animal experiment article blue light brain cortex electroencephalogram female in vivo study laser mouse nonhuman Animals Cerebral Cortex Female gamma-Aminobutyric Acid Mice Neural Inhibition Neurons Photolysis Mus
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_0100879X_v44_n7_p688_LopesdosSantos
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_0100879X_v44_n7_p688_LopesdosSantos
record_format	dspace
spelling	todo:paper_0100879X_v44_n7_p688_LopesdosSantos2023-10-03T14:57:21Z In vivo photorelease of GABA in the mouse cortex Lopes-dos-Santos, V. Campi, J. Filevich, O. Ribeiro, S. Etchenique, R. Caged compounds Cerebral cortex GABA Neural stimulation Photorelease 4 aminobutyric acid 4 aminobutyric acid release animal experiment article blue light brain cortex electroencephalogram female in vivo study laser mouse nonhuman Animals Cerebral Cortex Female gamma-Aminobutyric Acid Mice Neural Inhibition Neurons Photolysis Mus Electrical stimulation has been used for more than 100 years in neuroscientific and biomedical research as a powerful tool for controlled perturbations of neural activity. Despite quickly driving neuronal activity, this technique presents some important limitations, such as the impossibility to activate or deactivate specific neuronal populations within a single stimulation site. This problem can be avoided by pharmacological methods based on the administration of receptor ligands able to cause specific changes in neuronal activity. However, intracerebral injections of neuroactive molecules inherently confound the dynamics of drug diffusion with receptor activation. Caged compounds have been proposed to circumvent this problem, for spatially and temporally controlled release of molecules. Caged compounds consist of a protecting group and a ligand made inactive by the bond between the two parts. By breaking this bond with light of an appropriate wavelength, the ligand recovers its activity within milliseconds. To test these compounds in vivo, we recorded local field potentials (LFPs) from the cerebral cortex of anesthetized female mice (CF1, 60-70 days, 20-30 g) before and after infusion with caged γ-amino-butyric-acid (GABA). After 30 min, we irradiated the cortical surface with pulses of blue light in order to photorelease the caged GABA and measure its effect on global brain activity. Laser pulses significantly and consistently decreased LFP power in four different frequency bands with a precision of few milliseconds (P < 0.000001); however, the inhibitory effects lasted several minutes (P < 0.0043). The technical difficulties and limitations of neurotransmitter photorelease are presented, and perspectives for future in vivo applications of the method are discussed. Fil:Filevich, O. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Etchenique, R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_0100879X_v44_n7_p688_LopesdosSantos
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Caged compounds Cerebral cortex GABA Neural stimulation Photorelease 4 aminobutyric acid 4 aminobutyric acid release animal experiment article blue light brain cortex electroencephalogram female in vivo study laser mouse nonhuman Animals Cerebral Cortex Female gamma-Aminobutyric Acid Mice Neural Inhibition Neurons Photolysis Mus
spellingShingle	Caged compounds Cerebral cortex GABA Neural stimulation Photorelease 4 aminobutyric acid 4 aminobutyric acid release animal experiment article blue light brain cortex electroencephalogram female in vivo study laser mouse nonhuman Animals Cerebral Cortex Female gamma-Aminobutyric Acid Mice Neural Inhibition Neurons Photolysis Mus Lopes-dos-Santos, V. Campi, J. Filevich, O. Ribeiro, S. Etchenique, R. In vivo photorelease of GABA in the mouse cortex
topic_facet	Caged compounds Cerebral cortex GABA Neural stimulation Photorelease 4 aminobutyric acid 4 aminobutyric acid release animal experiment article blue light brain cortex electroencephalogram female in vivo study laser mouse nonhuman Animals Cerebral Cortex Female gamma-Aminobutyric Acid Mice Neural Inhibition Neurons Photolysis Mus
description	Electrical stimulation has been used for more than 100 years in neuroscientific and biomedical research as a powerful tool for controlled perturbations of neural activity. Despite quickly driving neuronal activity, this technique presents some important limitations, such as the impossibility to activate or deactivate specific neuronal populations within a single stimulation site. This problem can be avoided by pharmacological methods based on the administration of receptor ligands able to cause specific changes in neuronal activity. However, intracerebral injections of neuroactive molecules inherently confound the dynamics of drug diffusion with receptor activation. Caged compounds have been proposed to circumvent this problem, for spatially and temporally controlled release of molecules. Caged compounds consist of a protecting group and a ligand made inactive by the bond between the two parts. By breaking this bond with light of an appropriate wavelength, the ligand recovers its activity within milliseconds. To test these compounds in vivo, we recorded local field potentials (LFPs) from the cerebral cortex of anesthetized female mice (CF1, 60-70 days, 20-30 g) before and after infusion with caged γ-amino-butyric-acid (GABA). After 30 min, we irradiated the cortical surface with pulses of blue light in order to photorelease the caged GABA and measure its effect on global brain activity. Laser pulses significantly and consistently decreased LFP power in four different frequency bands with a precision of few milliseconds (P < 0.000001); however, the inhibitory effects lasted several minutes (P < 0.0043). The technical difficulties and limitations of neurotransmitter photorelease are presented, and perspectives for future in vivo applications of the method are discussed.
format	JOUR
author	Lopes-dos-Santos, V. Campi, J. Filevich, O. Ribeiro, S. Etchenique, R.
author_facet	Lopes-dos-Santos, V. Campi, J. Filevich, O. Ribeiro, S. Etchenique, R.
author_sort	Lopes-dos-Santos, V.
title	In vivo photorelease of GABA in the mouse cortex
title_short	In vivo photorelease of GABA in the mouse cortex
title_full	In vivo photorelease of GABA in the mouse cortex
title_fullStr	In vivo photorelease of GABA in the mouse cortex
title_full_unstemmed	In vivo photorelease of GABA in the mouse cortex
title_sort	in vivo photorelease of gaba in the mouse cortex
url	http://hdl.handle.net/20.500.12110/paper_0100879X_v44_n7_p688_LopesdosSantos
work_keys_str_mv	AT lopesdossantosv invivophotoreleaseofgabainthemousecortex AT campij invivophotoreleaseofgabainthemousecortex AT filevicho invivophotoreleaseofgabainthemousecortex AT ribeiros invivophotoreleaseofgabainthemousecortex AT etcheniquer invivophotoreleaseofgabainthemousecortex
_version_	1807318181370396672

In vivo photorelease of GABA in the mouse cortex

Ejemplares similares