Free and protein-linked glycoinositolphospholipids in Trypanosoma cruzi.

Two glycoinositol phospholipids (GIPL A and GIPL B) have been purified from epimastigotes of Trypanosoma cruzi at the logarithmic phase of growth (2 days). The GIPLs differ mainly in the lipid moiety and are similar to the lipopeptidophosphoglycan (LPPG) previously isolated from epimastigotes at the...

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Autor principal: de Lederkremer, R.M.
Formato: JOUR
Materias:
fatty acid
glycolipid
glycosylphosphatidylinositol
phosphatidylinositol
protozoal protein
animal
article
carbohydrate analysis
chemistry
metabolism
molecular genetics
Trypanosoma cruzi
Animals
Carbohydrate Sequence
Fatty Acids
Glycolipids
Glycosylphosphatidylinositols
Molecular Sequence Data
Phosphatidylinositols
Protozoan Proteins
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_0100879X_v27_n2_p239_deLederkremer
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_0100879X_v27_n2_p239_deLederkremer
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spelling todo:paper_0100879X_v27_n2_p239_deLederkremer2023-10-03T14:57:17Z Free and protein-linked glycoinositolphospholipids in Trypanosoma cruzi. de Lederkremer, R.M. fatty acid glycolipid glycosylphosphatidylinositol phosphatidylinositol protozoal protein animal article carbohydrate analysis chemistry metabolism molecular genetics Trypanosoma cruzi Animals Carbohydrate Sequence Fatty Acids Glycolipids Glycosylphosphatidylinositols Molecular Sequence Data Phosphatidylinositols Protozoan Proteins Trypanosoma cruzi Two glycoinositol phospholipids (GIPL A and GIPL B) have been purified from epimastigotes of Trypanosoma cruzi at the logarithmic phase of growth (2 days). The GIPLs differ mainly in the lipid moiety and are similar to the lipopeptidophosphoglycan (LPPG) previously isolated from epimastigotes at the stationary phase (4-5 days). [3H]-palmitic acid was incorporated into 1-O-hexadecyl-2-O-palmitoylglycerol in GIPL A and into a sphinganine ceramide with palmitic acid and lignoceric acid as the fatty acids in GIPL B. The lipids could be released by incubation with phosphatidylinositol-specific phospholipase C (PI-PLC) or glycosylphosphatidylinositol phospholipase D (GPI-PLD) from rat serum. The oligosaccharides share the common core structure of the glycosylphosphatidylinositol (GPI) membrane anchors. Microheterogeneity was demonstrated, as well as substitution by galactose, which is mainly in the furanose configuration as was previously described for the LPPG. However, methylation analysis indicated that 20% of the galactose is present as terminal pyranose units. In infective trypomastigotes, [3H]-palmitic acid was incorporated into the anchor of the Tc-85 glycoprotein. The lipid cleaved by phospholipase C digestion was identified as 1-O-hexadecylglycerol and the main oligosaccharide has the structure of the conserved core of all GPI anchors. [3H]-palmitic acid-labelled Tc-85 released into the culture medium as membrane vesicles showed 80% resistance to the action of PI-PLC. However, after mild alkaline hydrolysis, part of the radioactivity was released by the enzyme. Fil:de Lederkremer, R.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_0100879X_v27_n2_p239_deLederkremer
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic fatty acid
glycolipid
glycosylphosphatidylinositol
phosphatidylinositol
protozoal protein
animal
article
carbohydrate analysis
chemistry
metabolism
molecular genetics
Trypanosoma cruzi
Animals
Carbohydrate Sequence
Fatty Acids
Glycolipids
Glycosylphosphatidylinositols
Molecular Sequence Data
Phosphatidylinositols
Protozoan Proteins
Trypanosoma cruzi
spellingShingle fatty acid
glycolipid
glycosylphosphatidylinositol
phosphatidylinositol
protozoal protein
animal
article
carbohydrate analysis
chemistry
metabolism
molecular genetics
Trypanosoma cruzi
Animals
Carbohydrate Sequence
Fatty Acids
Glycolipids
Glycosylphosphatidylinositols
Molecular Sequence Data
Phosphatidylinositols
Protozoan Proteins
Trypanosoma cruzi
de Lederkremer, R.M.
Free and protein-linked glycoinositolphospholipids in Trypanosoma cruzi.
topic_facet fatty acid
glycolipid
glycosylphosphatidylinositol
phosphatidylinositol
protozoal protein
animal
article
carbohydrate analysis
chemistry
metabolism
molecular genetics
Trypanosoma cruzi
Animals
Carbohydrate Sequence
Fatty Acids
Glycolipids
Glycosylphosphatidylinositols
Molecular Sequence Data
Phosphatidylinositols
Protozoan Proteins
Trypanosoma cruzi
description Two glycoinositol phospholipids (GIPL A and GIPL B) have been purified from epimastigotes of Trypanosoma cruzi at the logarithmic phase of growth (2 days). The GIPLs differ mainly in the lipid moiety and are similar to the lipopeptidophosphoglycan (LPPG) previously isolated from epimastigotes at the stationary phase (4-5 days). [3H]-palmitic acid was incorporated into 1-O-hexadecyl-2-O-palmitoylglycerol in GIPL A and into a sphinganine ceramide with palmitic acid and lignoceric acid as the fatty acids in GIPL B. The lipids could be released by incubation with phosphatidylinositol-specific phospholipase C (PI-PLC) or glycosylphosphatidylinositol phospholipase D (GPI-PLD) from rat serum. The oligosaccharides share the common core structure of the glycosylphosphatidylinositol (GPI) membrane anchors. Microheterogeneity was demonstrated, as well as substitution by galactose, which is mainly in the furanose configuration as was previously described for the LPPG. However, methylation analysis indicated that 20% of the galactose is present as terminal pyranose units. In infective trypomastigotes, [3H]-palmitic acid was incorporated into the anchor of the Tc-85 glycoprotein. The lipid cleaved by phospholipase C digestion was identified as 1-O-hexadecylglycerol and the main oligosaccharide has the structure of the conserved core of all GPI anchors. [3H]-palmitic acid-labelled Tc-85 released into the culture medium as membrane vesicles showed 80% resistance to the action of PI-PLC. However, after mild alkaline hydrolysis, part of the radioactivity was released by the enzyme.
format JOUR
author de Lederkremer, R.M.
author_facet de Lederkremer, R.M.
author_sort de Lederkremer, R.M.
title Free and protein-linked glycoinositolphospholipids in Trypanosoma cruzi.
title_short Free and protein-linked glycoinositolphospholipids in Trypanosoma cruzi.
title_full Free and protein-linked glycoinositolphospholipids in Trypanosoma cruzi.
title_fullStr Free and protein-linked glycoinositolphospholipids in Trypanosoma cruzi.
title_full_unstemmed Free and protein-linked glycoinositolphospholipids in Trypanosoma cruzi.
title_sort free and protein-linked glycoinositolphospholipids in trypanosoma cruzi.
url http://hdl.handle.net/20.500.12110/paper_0100879X_v27_n2_p239_deLederkremer
work_keys_str_mv AT delederkremerrm freeandproteinlinkedglycoinositolphospholipidsintrypanosomacruzi
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