A new player in the biorefineries field: Phasin PhaP enhances tolerance to solvents and boosts ethanol and 1,3-propanediol synthesis in Escherichia coli
The microbial production of biofuels and other added-value chemicals is often limited by the intrinsic toxicity of these compounds. The phasin PhaP from the soil bacterium Azotobacter sp. strain FA8 is a polyhydroxyalkanoate granuleassociated protein that protects recombinant Escherichia coli agains...
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todo:paper_00992240_v83_n14_p_Mezzina2023-10-03T14:57:13Z A new player in the biorefineries field: Phasin PhaP enhances tolerance to solvents and boosts ethanol and 1,3-propanediol synthesis in Escherichia coli Mezzina, M.P. Álvarez, D.S. Egoburo, D.E. Peña, R.D. Nikel, P.I. Pettinari, M.J. 1,3-propanediol Butanol Chaperone Escherichia coli Ethanol GroEL Metabolic engineering PhaP Biofuels Biosynthesis Butenes Escherichia coli Ethanol Metabolic engineering Recombinant proteins 1 ,3 propanediol Chaperone Granule-associated proteins GroEL Heterologous expression PhaP Poly-3-hydroxybutyrate Recombinant Escherichia coli Organic solvents bioengineering biofuel biomass coliform bacterium ethanol fitness gene expression growth rate protein recombination solvent Azotobacter Bacteria (microorganisms) Escherichia coli 1,3-propanediol alcohol bacterial protein biofuel phasin plant lectin propanediol derivative Azotobacter Escherichia coli genetics metabolism Azotobacter Bacterial Proteins Biofuels Escherichia coli Ethanol Plant Lectins Propylene Glycols The microbial production of biofuels and other added-value chemicals is often limited by the intrinsic toxicity of these compounds. The phasin PhaP from the soil bacterium Azotobacter sp. strain FA8 is a polyhydroxyalkanoate granuleassociated protein that protects recombinant Escherichia coli against several kinds of stress. PhaP enhances growth and poly(3-hydroxybutyrate) synthesis in polymerproducing recombinant strains and reduces the formation of inclusion bodies during overproduction of heterologous proteins. In this work, the heterologous expression of this phasin in E. coli was used as a strategy to increase tolerance to several biotechnologically relevant chemicals. PhaP was observed to enhance bacterial fitness in the presence of biofuels, such as ethanol and butanol, and other chemicals, such as 1,3-propanediol. The effect of PhaP was also studied in a groELS mutant strain, in which both GroELS and PhaP were observed to exert a beneficial effect that varied depending on the chemical tested. Lastly, the potential of PhaP and GroEL to enhance the accumulation of ethanol or 1,3-propanediol was analyzed in recombinant E. coli. Strains that overexpressed either groEL or phaP had increased growth, reflected in a higher final biomass and product titer than the control strain. Taken together, these results add a novel application to the already multifaceted phasin protein group, suggesting that expression of these proteins or other chaperones can be used to improve the production of biofuels and other chemicals. © 2017 American Society for Microbiology. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00992240_v83_n14_p_Mezzina |
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Universidad de Buenos Aires |
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I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
1,3-propanediol Butanol Chaperone Escherichia coli Ethanol GroEL Metabolic engineering PhaP Biofuels Biosynthesis Butenes Escherichia coli Ethanol Metabolic engineering Recombinant proteins 1 ,3 propanediol Chaperone Granule-associated proteins GroEL Heterologous expression PhaP Poly-3-hydroxybutyrate Recombinant Escherichia coli Organic solvents bioengineering biofuel biomass coliform bacterium ethanol fitness gene expression growth rate protein recombination solvent Azotobacter Bacteria (microorganisms) Escherichia coli 1,3-propanediol alcohol bacterial protein biofuel phasin plant lectin propanediol derivative Azotobacter Escherichia coli genetics metabolism Azotobacter Bacterial Proteins Biofuels Escherichia coli Ethanol Plant Lectins Propylene Glycols |
| spellingShingle |
1,3-propanediol Butanol Chaperone Escherichia coli Ethanol GroEL Metabolic engineering PhaP Biofuels Biosynthesis Butenes Escherichia coli Ethanol Metabolic engineering Recombinant proteins 1 ,3 propanediol Chaperone Granule-associated proteins GroEL Heterologous expression PhaP Poly-3-hydroxybutyrate Recombinant Escherichia coli Organic solvents bioengineering biofuel biomass coliform bacterium ethanol fitness gene expression growth rate protein recombination solvent Azotobacter Bacteria (microorganisms) Escherichia coli 1,3-propanediol alcohol bacterial protein biofuel phasin plant lectin propanediol derivative Azotobacter Escherichia coli genetics metabolism Azotobacter Bacterial Proteins Biofuels Escherichia coli Ethanol Plant Lectins Propylene Glycols Mezzina, M.P. Álvarez, D.S. Egoburo, D.E. Peña, R.D. Nikel, P.I. Pettinari, M.J. A new player in the biorefineries field: Phasin PhaP enhances tolerance to solvents and boosts ethanol and 1,3-propanediol synthesis in Escherichia coli |
| topic_facet |
1,3-propanediol Butanol Chaperone Escherichia coli Ethanol GroEL Metabolic engineering PhaP Biofuels Biosynthesis Butenes Escherichia coli Ethanol Metabolic engineering Recombinant proteins 1 ,3 propanediol Chaperone Granule-associated proteins GroEL Heterologous expression PhaP Poly-3-hydroxybutyrate Recombinant Escherichia coli Organic solvents bioengineering biofuel biomass coliform bacterium ethanol fitness gene expression growth rate protein recombination solvent Azotobacter Bacteria (microorganisms) Escherichia coli 1,3-propanediol alcohol bacterial protein biofuel phasin plant lectin propanediol derivative Azotobacter Escherichia coli genetics metabolism Azotobacter Bacterial Proteins Biofuels Escherichia coli Ethanol Plant Lectins Propylene Glycols |
| description |
The microbial production of biofuels and other added-value chemicals is often limited by the intrinsic toxicity of these compounds. The phasin PhaP from the soil bacterium Azotobacter sp. strain FA8 is a polyhydroxyalkanoate granuleassociated protein that protects recombinant Escherichia coli against several kinds of stress. PhaP enhances growth and poly(3-hydroxybutyrate) synthesis in polymerproducing recombinant strains and reduces the formation of inclusion bodies during overproduction of heterologous proteins. In this work, the heterologous expression of this phasin in E. coli was used as a strategy to increase tolerance to several biotechnologically relevant chemicals. PhaP was observed to enhance bacterial fitness in the presence of biofuels, such as ethanol and butanol, and other chemicals, such as 1,3-propanediol. The effect of PhaP was also studied in a groELS mutant strain, in which both GroELS and PhaP were observed to exert a beneficial effect that varied depending on the chemical tested. Lastly, the potential of PhaP and GroEL to enhance the accumulation of ethanol or 1,3-propanediol was analyzed in recombinant E. coli. Strains that overexpressed either groEL or phaP had increased growth, reflected in a higher final biomass and product titer than the control strain. Taken together, these results add a novel application to the already multifaceted phasin protein group, suggesting that expression of these proteins or other chaperones can be used to improve the production of biofuels and other chemicals. © 2017 American Society for Microbiology. |
| format |
JOUR |
| author |
Mezzina, M.P. Álvarez, D.S. Egoburo, D.E. Peña, R.D. Nikel, P.I. Pettinari, M.J. |
| author_facet |
Mezzina, M.P. Álvarez, D.S. Egoburo, D.E. Peña, R.D. Nikel, P.I. Pettinari, M.J. |
| author_sort |
Mezzina, M.P. |
| title |
A new player in the biorefineries field: Phasin PhaP enhances tolerance to solvents and boosts ethanol and 1,3-propanediol synthesis in Escherichia coli |
| title_short |
A new player in the biorefineries field: Phasin PhaP enhances tolerance to solvents and boosts ethanol and 1,3-propanediol synthesis in Escherichia coli |
| title_full |
A new player in the biorefineries field: Phasin PhaP enhances tolerance to solvents and boosts ethanol and 1,3-propanediol synthesis in Escherichia coli |
| title_fullStr |
A new player in the biorefineries field: Phasin PhaP enhances tolerance to solvents and boosts ethanol and 1,3-propanediol synthesis in Escherichia coli |
| title_full_unstemmed |
A new player in the biorefineries field: Phasin PhaP enhances tolerance to solvents and boosts ethanol and 1,3-propanediol synthesis in Escherichia coli |
| title_sort |
new player in the biorefineries field: phasin phap enhances tolerance to solvents and boosts ethanol and 1,3-propanediol synthesis in escherichia coli |
| url |
http://hdl.handle.net/20.500.12110/paper_00992240_v83_n14_p_Mezzina |
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