An alternate solution of fluorescence recovery kinetics after spot-bleaching for measuring diffusion coefficients. 2. Diffusion of fluorescein in aqueous sucrose solutions

The traditional analysis of the fluorescence recovery kinetics after spot bleaching yields expressions for the diffusion coefficient of the probe that are not suitable for linear fittings. In a previous work we developed an improved recovery function that is a better alternative for data analysis. T...

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Autores principales:	Corti, H.R., Frank, G.A., Marconi, M.C.
Formato:	JOUR
Materias:	Diffusion Fluorescein Fluorescence recovery Photobleaching Stokes-Einstein equation Sucrose solutions Supercooled
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_00959782_v37_n11_p1593_Corti
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_00959782_v37_n11_p1593_Corti
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spelling	todo:paper_00959782_v37_n11_p1593_Corti2023-10-03T14:56:43Z An alternate solution of fluorescence recovery kinetics after spot-bleaching for measuring diffusion coefficients. 2. Diffusion of fluorescein in aqueous sucrose solutions Corti, H.R. Frank, G.A. Marconi, M.C. Diffusion Fluorescein Fluorescence recovery Photobleaching Stokes-Einstein equation Sucrose solutions Supercooled The traditional analysis of the fluorescence recovery kinetics after spot bleaching yields expressions for the diffusion coefficient of the probe that are not suitable for linear fittings. In a previous work we developed an improved recovery function that is a better alternative for data analysis. To illustrate its application to real cases and compare it with the previous data treatment, we measured the time response of fluorescein in aqueous sucrose solutions, covering the unsaturated and the supercooled region, where decoupling between diffusion and viscosity is observed. The results are compared with the mobility of different types of solutes in aqueous sucrose solutions and are discussed in terms of the classical hydrodynamic model. © 2008 Springer Science+Business Media, LLC. Fil:Corti, H.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Frank, G.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Marconi, M.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00959782_v37_n11_p1593_Corti
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Diffusion Fluorescein Fluorescence recovery Photobleaching Stokes-Einstein equation Sucrose solutions Supercooled
spellingShingle	Diffusion Fluorescein Fluorescence recovery Photobleaching Stokes-Einstein equation Sucrose solutions Supercooled Corti, H.R. Frank, G.A. Marconi, M.C. An alternate solution of fluorescence recovery kinetics after spot-bleaching for measuring diffusion coefficients. 2. Diffusion of fluorescein in aqueous sucrose solutions
topic_facet	Diffusion Fluorescein Fluorescence recovery Photobleaching Stokes-Einstein equation Sucrose solutions Supercooled
description	The traditional analysis of the fluorescence recovery kinetics after spot bleaching yields expressions for the diffusion coefficient of the probe that are not suitable for linear fittings. In a previous work we developed an improved recovery function that is a better alternative for data analysis. To illustrate its application to real cases and compare it with the previous data treatment, we measured the time response of fluorescein in aqueous sucrose solutions, covering the unsaturated and the supercooled region, where decoupling between diffusion and viscosity is observed. The results are compared with the mobility of different types of solutes in aqueous sucrose solutions and are discussed in terms of the classical hydrodynamic model. © 2008 Springer Science+Business Media, LLC.
format	JOUR
author	Corti, H.R. Frank, G.A. Marconi, M.C.
author_facet	Corti, H.R. Frank, G.A. Marconi, M.C.
author_sort	Corti, H.R.
title	An alternate solution of fluorescence recovery kinetics after spot-bleaching for measuring diffusion coefficients. 2. Diffusion of fluorescein in aqueous sucrose solutions
title_short	An alternate solution of fluorescence recovery kinetics after spot-bleaching for measuring diffusion coefficients. 2. Diffusion of fluorescein in aqueous sucrose solutions
title_full	An alternate solution of fluorescence recovery kinetics after spot-bleaching for measuring diffusion coefficients. 2. Diffusion of fluorescein in aqueous sucrose solutions
title_fullStr	An alternate solution of fluorescence recovery kinetics after spot-bleaching for measuring diffusion coefficients. 2. Diffusion of fluorescein in aqueous sucrose solutions
title_full_unstemmed	An alternate solution of fluorescence recovery kinetics after spot-bleaching for measuring diffusion coefficients. 2. Diffusion of fluorescein in aqueous sucrose solutions
title_sort	alternate solution of fluorescence recovery kinetics after spot-bleaching for measuring diffusion coefficients. 2. diffusion of fluorescein in aqueous sucrose solutions
url	http://hdl.handle.net/20.500.12110/paper_00959782_v37_n11_p1593_Corti
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An alternate solution of fluorescence recovery kinetics after spot-bleaching for measuring diffusion coefficients. 2. Diffusion of fluorescein in aqueous sucrose solutions

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