An alternate solution of the fluorescence recovery kinetics after spot-bleaching for measuring diffusion coefficients. 1. Theory and numerical analysis

The classical three-point method for obtaining a diffusion coefficient of probes from the fluorescence recovery function in the Fluorescence Recovery After Photobleaching (FRAP) technique, when Gaussian or uniform spot-photobleaching profiles are used, leads to an expression that is not suitable for...

Descripción completa

Detalles Bibliográficos
Autores principales: Frank, G.A., Marconi, M.C., Corti, H.R.
Formato: JOUR
Materias:
Diffusion
Fluorescence recovery
FRAP
Photobleaching
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00959782_v37_n11_p1575_Frank
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_00959782_v37_n11_p1575_Frank
record_format dspace
spelling todo:paper_00959782_v37_n11_p1575_Frank2023-10-03T14:56:43Z An alternate solution of the fluorescence recovery kinetics after spot-bleaching for measuring diffusion coefficients. 1. Theory and numerical analysis Frank, G.A. Marconi, M.C. Corti, H.R. Diffusion Fluorescence recovery FRAP Photobleaching The classical three-point method for obtaining a diffusion coefficient of probes from the fluorescence recovery function in the Fluorescence Recovery After Photobleaching (FRAP) technique, when Gaussian or uniform spot-photobleaching profiles are used, leads to an expression that is not suitable for linear fittings. Therefore, determination of the diffusion coefficient is complex and very dependent on the recovery time. In this work we propose a new solution for the fluorescence recovery function after spot-bleaching using a Gaussian beam, which is a better alternative method than the three-point method for the data analysis of the FRAP kinetics. The new method can be applied for shorter recovery times thereby minimizing errors due to convective effects. It leads to a linear relationship between the recovery function and the diffusion coefficient and, as shown in the analysis of a model case, allows a more accurate determination of the diffusion coefficient of fluorescent probes. © 2008 Springer Science+Business Media, LLC. Fil:Frank, G.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Marconi, M.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Corti, H.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00959782_v37_n11_p1575_Frank
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Diffusion
Fluorescence recovery
FRAP
Photobleaching
spellingShingle Diffusion
Fluorescence recovery
FRAP
Photobleaching
Frank, G.A.
Marconi, M.C.
Corti, H.R.
An alternate solution of the fluorescence recovery kinetics after spot-bleaching for measuring diffusion coefficients. 1. Theory and numerical analysis
topic_facet Diffusion
Fluorescence recovery
FRAP
Photobleaching
description The classical three-point method for obtaining a diffusion coefficient of probes from the fluorescence recovery function in the Fluorescence Recovery After Photobleaching (FRAP) technique, when Gaussian or uniform spot-photobleaching profiles are used, leads to an expression that is not suitable for linear fittings. Therefore, determination of the diffusion coefficient is complex and very dependent on the recovery time. In this work we propose a new solution for the fluorescence recovery function after spot-bleaching using a Gaussian beam, which is a better alternative method than the three-point method for the data analysis of the FRAP kinetics. The new method can be applied for shorter recovery times thereby minimizing errors due to convective effects. It leads to a linear relationship between the recovery function and the diffusion coefficient and, as shown in the analysis of a model case, allows a more accurate determination of the diffusion coefficient of fluorescent probes. © 2008 Springer Science+Business Media, LLC.
format JOUR
author Frank, G.A.
Marconi, M.C.
Corti, H.R.
author_facet Frank, G.A.
Marconi, M.C.
Corti, H.R.
author_sort Frank, G.A.
title An alternate solution of the fluorescence recovery kinetics after spot-bleaching for measuring diffusion coefficients. 1. Theory and numerical analysis
title_short An alternate solution of the fluorescence recovery kinetics after spot-bleaching for measuring diffusion coefficients. 1. Theory and numerical analysis
title_full An alternate solution of the fluorescence recovery kinetics after spot-bleaching for measuring diffusion coefficients. 1. Theory and numerical analysis
title_fullStr An alternate solution of the fluorescence recovery kinetics after spot-bleaching for measuring diffusion coefficients. 1. Theory and numerical analysis
title_full_unstemmed An alternate solution of the fluorescence recovery kinetics after spot-bleaching for measuring diffusion coefficients. 1. Theory and numerical analysis
title_sort alternate solution of the fluorescence recovery kinetics after spot-bleaching for measuring diffusion coefficients. 1. theory and numerical analysis
url http://hdl.handle.net/20.500.12110/paper_00959782_v37_n11_p1575_Frank
work_keys_str_mv AT frankga analternatesolutionofthefluorescencerecoverykineticsafterspotbleachingformeasuringdiffusioncoefficients1theoryandnumericalanalysis
AT marconimc analternatesolutionofthefluorescencerecoverykineticsafterspotbleachingformeasuringdiffusioncoefficients1theoryandnumericalanalysis
AT cortihr analternatesolutionofthefluorescencerecoverykineticsafterspotbleachingformeasuringdiffusioncoefficients1theoryandnumericalanalysis
AT frankga alternatesolutionofthefluorescencerecoverykineticsafterspotbleachingformeasuringdiffusioncoefficients1theoryandnumericalanalysis
AT marconimc alternatesolutionofthefluorescencerecoverykineticsafterspotbleachingformeasuringdiffusioncoefficients1theoryandnumericalanalysis
AT cortihr alternatesolutionofthefluorescencerecoverykineticsafterspotbleachingformeasuringdiffusioncoefficients1theoryandnumericalanalysis
_version_ 1807315069430661120

Ejemplares similares