Flow cytometry to evaluate Anaplasma marginale parasitemia using a fluorescent nucleic acid stain

In this work we describe a flow cytometry-based method using SYTO16 (a DNA intercalating agent) to quantify Anaplasma marginale-infected erythrocytes in blood from bovine animals. The linearity and reproducibility of the results obtained with SYTO16 labeling followed by flow cytometry analysis make...

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Detalles Bibliográficos
Autores principales: Moretta, R., Ruybal, P., Mesplet, M., Petrigh, R., Nuñez, P., Gil, G., Wilkowsky, S., Garbossa, G., Farber, M.
Formato: SER
Materias:
Anaplasma marginale
Flow cytometry
Parasitemia
SYTO16
animal cell
cattle
conference paper
controlled study
erythrocyte
flow cytometry
measurement
nonhuman
parasite examination
parasitemia
reproducibility
Animalia
Bovinae
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00778923_v1149_n_p111_Moretta
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
Descripción
Sumario:In this work we describe a flow cytometry-based method using SYTO16 (a DNA intercalating agent) to quantify Anaplasma marginale-infected erythrocytes in blood from bovine animals. The linearity and reproducibility of the results obtained with SYTO16 labeling followed by flow cytometry analysis make it a suitable approach for measurement of parasitemia in A. marginale infections. © 2008 New York Academy of Sciences.

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