Flow cytometry to evaluate Anaplasma marginale parasitemia using a fluorescent nucleic acid stain

In this work we describe a flow cytometry-based method using SYTO16 (a DNA intercalating agent) to quantify Anaplasma marginale-infected erythrocytes in blood from bovine animals. The linearity and reproducibility of the results obtained with SYTO16 labeling followed by flow cytometry analysis make...

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Autores principales: Moretta, R., Ruybal, P., Mesplet, M., Petrigh, R., Nuñez, P., Gil, G., Wilkowsky, S., Garbossa, G., Farber, M.
Formato: SER
Materias:
Anaplasma marginale
Flow cytometry
Parasitemia
SYTO16
animal cell
cattle
conference paper
controlled study
erythrocyte
flow cytometry
measurement
nonhuman
parasite examination
parasitemia
reproducibility
Animalia
Bovinae
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00778923_v1149_n_p111_Moretta
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_00778923_v1149_n_p111_Moretta
record_format dspace
spelling todo:paper_00778923_v1149_n_p111_Moretta2023-10-03T14:54:19Z Flow cytometry to evaluate Anaplasma marginale parasitemia using a fluorescent nucleic acid stain Moretta, R. Ruybal, P. Mesplet, M. Petrigh, R. Nuñez, P. Gil, G. Wilkowsky, S. Garbossa, G. Farber, M. Anaplasma marginale Flow cytometry Parasitemia SYTO16 Anaplasma marginale animal cell cattle conference paper controlled study erythrocyte flow cytometry measurement nonhuman parasite examination parasitemia reproducibility Anaplasma marginale Animalia Bovinae In this work we describe a flow cytometry-based method using SYTO16 (a DNA intercalating agent) to quantify Anaplasma marginale-infected erythrocytes in blood from bovine animals. The linearity and reproducibility of the results obtained with SYTO16 labeling followed by flow cytometry analysis make it a suitable approach for measurement of parasitemia in A. marginale infections. © 2008 New York Academy of Sciences. Fil:Moretta, R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ruybal, P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Petrigh, R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Nuñez, P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Wilkowsky, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Garbossa, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Farber, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. SER info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00778923_v1149_n_p111_Moretta
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Anaplasma marginale
Flow cytometry
Parasitemia
SYTO16
Anaplasma marginale
animal cell
cattle
conference paper
controlled study
erythrocyte
flow cytometry
measurement
nonhuman
parasite examination
parasitemia
reproducibility
Anaplasma marginale
Animalia
Bovinae
spellingShingle Anaplasma marginale
Flow cytometry
Parasitemia
SYTO16
Anaplasma marginale
animal cell
cattle
conference paper
controlled study
erythrocyte
flow cytometry
measurement
nonhuman
parasite examination
parasitemia
reproducibility
Anaplasma marginale
Animalia
Bovinae
Moretta, R.
Ruybal, P.
Mesplet, M.
Petrigh, R.
Nuñez, P.
Gil, G.
Wilkowsky, S.
Garbossa, G.
Farber, M.
Flow cytometry to evaluate Anaplasma marginale parasitemia using a fluorescent nucleic acid stain
topic_facet Anaplasma marginale
Flow cytometry
Parasitemia
SYTO16
Anaplasma marginale
animal cell
cattle
conference paper
controlled study
erythrocyte
flow cytometry
measurement
nonhuman
parasite examination
parasitemia
reproducibility
Anaplasma marginale
Animalia
Bovinae
description In this work we describe a flow cytometry-based method using SYTO16 (a DNA intercalating agent) to quantify Anaplasma marginale-infected erythrocytes in blood from bovine animals. The linearity and reproducibility of the results obtained with SYTO16 labeling followed by flow cytometry analysis make it a suitable approach for measurement of parasitemia in A. marginale infections. © 2008 New York Academy of Sciences.
format SER
author Moretta, R.
Ruybal, P.
Mesplet, M.
Petrigh, R.
Nuñez, P.
Gil, G.
Wilkowsky, S.
Garbossa, G.
Farber, M.
author_facet Moretta, R.
Ruybal, P.
Mesplet, M.
Petrigh, R.
Nuñez, P.
Gil, G.
Wilkowsky, S.
Garbossa, G.
Farber, M.
author_sort Moretta, R.
title Flow cytometry to evaluate Anaplasma marginale parasitemia using a fluorescent nucleic acid stain
title_short Flow cytometry to evaluate Anaplasma marginale parasitemia using a fluorescent nucleic acid stain
title_full Flow cytometry to evaluate Anaplasma marginale parasitemia using a fluorescent nucleic acid stain
title_fullStr Flow cytometry to evaluate Anaplasma marginale parasitemia using a fluorescent nucleic acid stain
title_full_unstemmed Flow cytometry to evaluate Anaplasma marginale parasitemia using a fluorescent nucleic acid stain
title_sort flow cytometry to evaluate anaplasma marginale parasitemia using a fluorescent nucleic acid stain
url http://hdl.handle.net/20.500.12110/paper_00778923_v1149_n_p111_Moretta
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