Lipids Shed into the Culture Medium by Trypomastigotes of Trypanosoma cruzi
Trypomastigote forms of Trypanosoma cruzi were metabolically labeled with [14C]-ethanolamine and [3H]-palmitic acid. Lipids shed to the culture medium were analyzed and compared with the parasite components. Phosphatidylcholine and lysophosphatidylcholine accounted for 53% of the total incorporated...
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todo:paper_00740276_v95_n1-2_p97_Agusti2023-10-03T14:53:47Z Lipids Shed into the Culture Medium by Trypomastigotes of Trypanosoma cruzi Agusti, R. Couto, A.S. Alves, M.J.M. Colli, W. De Lederkremer, R.M. Desaturated fatty acids Phospholipids [14C]-ethanolamine and [3H]-palmitic acid incorporation Trypanosoma Trypanosoma cruzi ethanolamine derivative fatty acid lipid lysophosphatidylcholine palmitic acid phosphatidylcholine unsaturated fatty acid animal article chemistry culture medium lipid metabolism metabolism thin layer chromatography Trypanosoma cruzi Animals Chromatography, Thin Layer Culture Media Ethanolamines Fatty Acids, Nonesterified Fatty Acids, Unsaturated Lipid Metabolism Lipids Lysophosphatidylcholines Palmitic Acid Phosphatidylcholines Trypanosoma cruzi Trypomastigote forms of Trypanosoma cruzi were metabolically labeled with [14C]-ethanolamine and [3H]-palmitic acid. Lipids shed to the culture medium were analyzed and compared with the parasite components. Phosphatidylcholine and lysophosphatidylcholine accounted for 53% of the total incorporated precursor. Interestingly, phosphatidylethanolamine and its lyso derivative lysophosphatidylethanolamine, although present in significant amounts in the parasites, could not be detected in the shed material. Shed lipids were highly enriched in the desaturated fatty acids C16:1 and C18:1 when compared to the total fatty acid pool isolated from the parasites. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00740276_v95_n1-2_p97_Agusti |
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Universidad de Buenos Aires |
| institution_str |
I-28 |
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R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Desaturated fatty acids Phospholipids [14C]-ethanolamine and [3H]-palmitic acid incorporation Trypanosoma Trypanosoma cruzi ethanolamine derivative fatty acid lipid lysophosphatidylcholine palmitic acid phosphatidylcholine unsaturated fatty acid animal article chemistry culture medium lipid metabolism metabolism thin layer chromatography Trypanosoma cruzi Animals Chromatography, Thin Layer Culture Media Ethanolamines Fatty Acids, Nonesterified Fatty Acids, Unsaturated Lipid Metabolism Lipids Lysophosphatidylcholines Palmitic Acid Phosphatidylcholines Trypanosoma cruzi |
| spellingShingle |
Desaturated fatty acids Phospholipids [14C]-ethanolamine and [3H]-palmitic acid incorporation Trypanosoma Trypanosoma cruzi ethanolamine derivative fatty acid lipid lysophosphatidylcholine palmitic acid phosphatidylcholine unsaturated fatty acid animal article chemistry culture medium lipid metabolism metabolism thin layer chromatography Trypanosoma cruzi Animals Chromatography, Thin Layer Culture Media Ethanolamines Fatty Acids, Nonesterified Fatty Acids, Unsaturated Lipid Metabolism Lipids Lysophosphatidylcholines Palmitic Acid Phosphatidylcholines Trypanosoma cruzi Agusti, R. Couto, A.S. Alves, M.J.M. Colli, W. De Lederkremer, R.M. Lipids Shed into the Culture Medium by Trypomastigotes of Trypanosoma cruzi |
| topic_facet |
Desaturated fatty acids Phospholipids [14C]-ethanolamine and [3H]-palmitic acid incorporation Trypanosoma Trypanosoma cruzi ethanolamine derivative fatty acid lipid lysophosphatidylcholine palmitic acid phosphatidylcholine unsaturated fatty acid animal article chemistry culture medium lipid metabolism metabolism thin layer chromatography Trypanosoma cruzi Animals Chromatography, Thin Layer Culture Media Ethanolamines Fatty Acids, Nonesterified Fatty Acids, Unsaturated Lipid Metabolism Lipids Lysophosphatidylcholines Palmitic Acid Phosphatidylcholines Trypanosoma cruzi |
| description |
Trypomastigote forms of Trypanosoma cruzi were metabolically labeled with [14C]-ethanolamine and [3H]-palmitic acid. Lipids shed to the culture medium were analyzed and compared with the parasite components. Phosphatidylcholine and lysophosphatidylcholine accounted for 53% of the total incorporated precursor. Interestingly, phosphatidylethanolamine and its lyso derivative lysophosphatidylethanolamine, although present in significant amounts in the parasites, could not be detected in the shed material. Shed lipids were highly enriched in the desaturated fatty acids C16:1 and C18:1 when compared to the total fatty acid pool isolated from the parasites. |
| format |
JOUR |
| author |
Agusti, R. Couto, A.S. Alves, M.J.M. Colli, W. De Lederkremer, R.M. |
| author_facet |
Agusti, R. Couto, A.S. Alves, M.J.M. Colli, W. De Lederkremer, R.M. |
| author_sort |
Agusti, R. |
| title |
Lipids Shed into the Culture Medium by Trypomastigotes of Trypanosoma cruzi |
| title_short |
Lipids Shed into the Culture Medium by Trypomastigotes of Trypanosoma cruzi |
| title_full |
Lipids Shed into the Culture Medium by Trypomastigotes of Trypanosoma cruzi |
| title_fullStr |
Lipids Shed into the Culture Medium by Trypomastigotes of Trypanosoma cruzi |
| title_full_unstemmed |
Lipids Shed into the Culture Medium by Trypomastigotes of Trypanosoma cruzi |
| title_sort |
lipids shed into the culture medium by trypomastigotes of trypanosoma cruzi |
| url |
http://hdl.handle.net/20.500.12110/paper_00740276_v95_n1-2_p97_Agusti |
| work_keys_str_mv |
AT agustir lipidsshedintotheculturemediumbytrypomastigotesoftrypanosomacruzi AT coutoas lipidsshedintotheculturemediumbytrypomastigotesoftrypanosomacruzi AT alvesmjm lipidsshedintotheculturemediumbytrypomastigotesoftrypanosomacruzi AT colliw lipidsshedintotheculturemediumbytrypomastigotesoftrypanosomacruzi AT delederkremerrm lipidsshedintotheculturemediumbytrypomastigotesoftrypanosomacruzi |
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