Towards the Physical Map of the Trypanosoma cruzi Nuclear Genome: Construction of YAC and BAC Libraries of the Reference Clone T. cruzi CL-Brener
Strategies to construct the physical map of the Trypanosoma cruzi nuclear genome have to capitalize on three main advantages of the parasite genome, namely (a) its small size, (b) the fact that all chromosomes can be defined, and many of them can be isolated by pulse field gel electrophoresis, and (...
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todo:paper_00740276_v92_n6_p843_Ferrari2023-10-03T14:53:45Z Towards the Physical Map of the Trypanosoma cruzi Nuclear Genome: Construction of YAC and BAC Libraries of the Reference Clone T. cruzi CL-Brener Ferrari, I. Lorenzi, H. Santos, M.R. Brandariz, S. Requena, J.M. Schijman, A. Vázquez, M. Da Silveira, J.F. Ben-Dov, C. Medrano, C. Ghío, S. López Bergami, P. Cano, I. Zingales, B. Urmenyi, T.P. Rondinelli, E. González, A. Cortes, A. Lopez, M.C. Thomas, M.C. Alonso, C. Ramírez, J.L. Chiurrillo, M.A. Rangel Aldao, R. Brandão, A. Degrave, W. Perrot, V. Saumier, M. Billaut, A. Cohen, D. Le Paslier, D. Levin, M.J. BAC library Expressed sequence tags (EST) Genome project Physical map Sequence tagged sites (STS) Trypanosoma cruzi YAC library Bacteria (microorganisms) Trypanosoma Trypanosoma cruzi animal article bacterial chromosome chromosome map cloning gene library genetics genome Trypanosoma cruzi yeast artificial chromosome Animals Chromosome Mapping Chromosomes, Artificial, Yeast Chromosomes, Bacterial Cloning, Organism Genome, Protozoan Genomic Library Trypanosoma cruzi Strategies to construct the physical map of the Trypanosoma cruzi nuclear genome have to capitalize on three main advantages of the parasite genome, namely (a) its small size, (b) the fact that all chromosomes can be defined, and many of them can be isolated by pulse field gel electrophoresis, and (c) the fact that simple Southern blots of electrophoretic karyotypes can be used to map sequence tagged sites and expressed sequence tags to chromosomal bands. A major drawback to cope with is the complexity of T. cruzi genetics, that hinders the construction of a comprehensive genetic map. As a first step towards physical mapping, we report the construction and partial characterization of a T. cruzi CL-Brener genomic library in yeast artificial chromosomes (YACs) that consists of 2,770 individual YACs with a mean insert size of 365 kb encompassing around 10 genomic equivalents. Two libraries in bacterial artificial chromosomes (BACs) have been constructed, BACI and BACII. Both libraries represent about three genome equivalents. A third BAC library (BAC III) is being constructed. YACs and BACs are invaluable tools for physical mapping. More generally, they have to be considered as a common resource for research in Chagas disease. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00740276_v92_n6_p843_Ferrari |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
BAC library Expressed sequence tags (EST) Genome project Physical map Sequence tagged sites (STS) Trypanosoma cruzi YAC library Bacteria (microorganisms) Trypanosoma Trypanosoma cruzi animal article bacterial chromosome chromosome map cloning gene library genetics genome Trypanosoma cruzi yeast artificial chromosome Animals Chromosome Mapping Chromosomes, Artificial, Yeast Chromosomes, Bacterial Cloning, Organism Genome, Protozoan Genomic Library Trypanosoma cruzi |
| spellingShingle |
BAC library Expressed sequence tags (EST) Genome project Physical map Sequence tagged sites (STS) Trypanosoma cruzi YAC library Bacteria (microorganisms) Trypanosoma Trypanosoma cruzi animal article bacterial chromosome chromosome map cloning gene library genetics genome Trypanosoma cruzi yeast artificial chromosome Animals Chromosome Mapping Chromosomes, Artificial, Yeast Chromosomes, Bacterial Cloning, Organism Genome, Protozoan Genomic Library Trypanosoma cruzi Ferrari, I. Lorenzi, H. Santos, M.R. Brandariz, S. Requena, J.M. Schijman, A. Vázquez, M. Da Silveira, J.F. Ben-Dov, C. Medrano, C. Ghío, S. López Bergami, P. Cano, I. Zingales, B. Urmenyi, T.P. Rondinelli, E. González, A. Cortes, A. Lopez, M.C. Thomas, M.C. Alonso, C. Ramírez, J.L. Chiurrillo, M.A. Rangel Aldao, R. Brandão, A. Degrave, W. Perrot, V. Saumier, M. Billaut, A. Cohen, D. Le Paslier, D. Levin, M.J. Towards the Physical Map of the Trypanosoma cruzi Nuclear Genome: Construction of YAC and BAC Libraries of the Reference Clone T. cruzi CL-Brener |
| topic_facet |
BAC library Expressed sequence tags (EST) Genome project Physical map Sequence tagged sites (STS) Trypanosoma cruzi YAC library Bacteria (microorganisms) Trypanosoma Trypanosoma cruzi animal article bacterial chromosome chromosome map cloning gene library genetics genome Trypanosoma cruzi yeast artificial chromosome Animals Chromosome Mapping Chromosomes, Artificial, Yeast Chromosomes, Bacterial Cloning, Organism Genome, Protozoan Genomic Library Trypanosoma cruzi |
| description |
Strategies to construct the physical map of the Trypanosoma cruzi nuclear genome have to capitalize on three main advantages of the parasite genome, namely (a) its small size, (b) the fact that all chromosomes can be defined, and many of them can be isolated by pulse field gel electrophoresis, and (c) the fact that simple Southern blots of electrophoretic karyotypes can be used to map sequence tagged sites and expressed sequence tags to chromosomal bands. A major drawback to cope with is the complexity of T. cruzi genetics, that hinders the construction of a comprehensive genetic map. As a first step towards physical mapping, we report the construction and partial characterization of a T. cruzi CL-Brener genomic library in yeast artificial chromosomes (YACs) that consists of 2,770 individual YACs with a mean insert size of 365 kb encompassing around 10 genomic equivalents. Two libraries in bacterial artificial chromosomes (BACs) have been constructed, BACI and BACII. Both libraries represent about three genome equivalents. A third BAC library (BAC III) is being constructed. YACs and BACs are invaluable tools for physical mapping. More generally, they have to be considered as a common resource for research in Chagas disease. |
| format |
JOUR |
| author |
Ferrari, I. Lorenzi, H. Santos, M.R. Brandariz, S. Requena, J.M. Schijman, A. Vázquez, M. Da Silveira, J.F. Ben-Dov, C. Medrano, C. Ghío, S. López Bergami, P. Cano, I. Zingales, B. Urmenyi, T.P. Rondinelli, E. González, A. Cortes, A. Lopez, M.C. Thomas, M.C. Alonso, C. Ramírez, J.L. Chiurrillo, M.A. Rangel Aldao, R. Brandão, A. Degrave, W. Perrot, V. Saumier, M. Billaut, A. Cohen, D. Le Paslier, D. Levin, M.J. |
| author_facet |
Ferrari, I. Lorenzi, H. Santos, M.R. Brandariz, S. Requena, J.M. Schijman, A. Vázquez, M. Da Silveira, J.F. Ben-Dov, C. Medrano, C. Ghío, S. López Bergami, P. Cano, I. Zingales, B. Urmenyi, T.P. Rondinelli, E. González, A. Cortes, A. Lopez, M.C. Thomas, M.C. Alonso, C. Ramírez, J.L. Chiurrillo, M.A. Rangel Aldao, R. Brandão, A. Degrave, W. Perrot, V. Saumier, M. Billaut, A. Cohen, D. Le Paslier, D. Levin, M.J. |
| author_sort |
Ferrari, I. |
| title |
Towards the Physical Map of the Trypanosoma cruzi Nuclear Genome: Construction of YAC and BAC Libraries of the Reference Clone T. cruzi CL-Brener |
| title_short |
Towards the Physical Map of the Trypanosoma cruzi Nuclear Genome: Construction of YAC and BAC Libraries of the Reference Clone T. cruzi CL-Brener |
| title_full |
Towards the Physical Map of the Trypanosoma cruzi Nuclear Genome: Construction of YAC and BAC Libraries of the Reference Clone T. cruzi CL-Brener |
| title_fullStr |
Towards the Physical Map of the Trypanosoma cruzi Nuclear Genome: Construction of YAC and BAC Libraries of the Reference Clone T. cruzi CL-Brener |
| title_full_unstemmed |
Towards the Physical Map of the Trypanosoma cruzi Nuclear Genome: Construction of YAC and BAC Libraries of the Reference Clone T. cruzi CL-Brener |
| title_sort |
towards the physical map of the trypanosoma cruzi nuclear genome: construction of yac and bac libraries of the reference clone t. cruzi cl-brener |
| url |
http://hdl.handle.net/20.500.12110/paper_00740276_v92_n6_p843_Ferrari |
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