Effects of Glyphosate on Somatic and Ovarian Growth in the Estuarine Crab Neohelice granulata, During the Pre-Reproductive Period

Adult females of the estuarine crab Neohelice granulata were exposed during the 3-month pre-reproductive period (winter) to the herbicide glyphosate, the most used pesticide in Argentina, at three different concentrations (0.02, 0.2, and 1 mg/L, as active ingredient). At the end of the in vivo assay...

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Autores principales:	Avigliano, L., Canosa, I.S., Medesani, D.A., Rodríguez, E.M.
Formato:	JOUR
Materias:	Estuarine crabs Glyphosate Oocytes Ovarian growth Reproduction Anthropometry Estuaries Shellfish Tissue Active ingredients Estuarine crab Glyphosates Gonadosomatic index Herbicide glyphosate Protein contents Herbicides glyphosate neurohormone vitellogenin cell crab growth reproduction weight adult animal experiment animal tissue Argentina Article assay body growth body weight gain controlled study estuarine species female hormone release in vitro study in vivo study Neohelice granulata nonhuman oocyte development ovary development protein content protein transport toxic concentration toxicity toxicity testing Decapoda (Crustacea)
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_00496979_v229_n2_p_Avigliano
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_00496979_v229_n2_p_Avigliano
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spelling	todo:paper_00496979_v229_n2_p_Avigliano2023-10-03T14:52:44Z Effects of Glyphosate on Somatic and Ovarian Growth in the Estuarine Crab Neohelice granulata, During the Pre-Reproductive Period Avigliano, L. Canosa, I.S. Medesani, D.A. Rodríguez, E.M. Estuarine crabs Glyphosate Oocytes Ovarian growth Reproduction Anthropometry Estuaries Shellfish Tissue Active ingredients Estuarine crab Glyphosates Gonadosomatic index Herbicide glyphosate Oocytes Protein contents Reproduction Herbicides glyphosate neurohormone vitellogenin cell crab glyphosate growth reproduction weight adult animal experiment animal tissue Argentina Article assay body growth body weight gain controlled study crab estuarine species female hormone release in vitro study in vivo study Neohelice granulata nonhuman oocyte development ovary development protein content protein transport reproduction toxic concentration toxicity toxicity testing Argentina Decapoda (Crustacea) Adult females of the estuarine crab Neohelice granulata were exposed during the 3-month pre-reproductive period (winter) to the herbicide glyphosate, the most used pesticide in Argentina, at three different concentrations (0.02, 0.2, and 1 mg/L, as active ingredient). At the end of the in vivo assay, the body weight gain and the ovarian growth were estimated, the last one in terms of the gonadosomatic index (GSI), the relative proportion of the different kind of oocytes, and their relative size. A decrease in the body weight gain was observed by effect of pure glyphosate, at all concentrations assayed. Although no differences in either the GSI or vitellogenic protein content of the ovary were noted between any glyphosate concentration and control, a higher proportion of reabsorbed vitellogenic oocytes was observed in the ovaries of crabs exposed to glyphosate at 1 mg/L, together with an increased area of previtellogenic oocytes. These effects were confirmed in vitro, at a glyphosate concentration of 0.2 mg/L. In fact, a higher area of previtellogenic oocytes was seen when glyphosate was added to the culture medium containing ovarian tissue, but a significant higher incidence of reabsorbed vitellogenic oocytes was seen only when eyestalk tissue was also added to the vials, suggesting that the secretion of some neurohormone involved in reabsorption is enhanced. The obtained results indicate that glyphosate is able to harm, in the studied species, both somatic and the ovarian growth. © 2018, Springer International Publishing AG, part of Springer Nature. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00496979_v229_n2_p_Avigliano
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Estuarine crabs Glyphosate Oocytes Ovarian growth Reproduction Anthropometry Estuaries Shellfish Tissue Active ingredients Estuarine crab Glyphosates Gonadosomatic index Herbicide glyphosate Oocytes Protein contents Reproduction Herbicides glyphosate neurohormone vitellogenin cell crab glyphosate growth reproduction weight adult animal experiment animal tissue Argentina Article assay body growth body weight gain controlled study crab estuarine species female hormone release in vitro study in vivo study Neohelice granulata nonhuman oocyte development ovary development protein content protein transport reproduction toxic concentration toxicity toxicity testing Argentina Decapoda (Crustacea)
spellingShingle	Estuarine crabs Glyphosate Oocytes Ovarian growth Reproduction Anthropometry Estuaries Shellfish Tissue Active ingredients Estuarine crab Glyphosates Gonadosomatic index Herbicide glyphosate Oocytes Protein contents Reproduction Herbicides glyphosate neurohormone vitellogenin cell crab glyphosate growth reproduction weight adult animal experiment animal tissue Argentina Article assay body growth body weight gain controlled study crab estuarine species female hormone release in vitro study in vivo study Neohelice granulata nonhuman oocyte development ovary development protein content protein transport reproduction toxic concentration toxicity toxicity testing Argentina Decapoda (Crustacea) Avigliano, L. Canosa, I.S. Medesani, D.A. Rodríguez, E.M. Effects of Glyphosate on Somatic and Ovarian Growth in the Estuarine Crab Neohelice granulata, During the Pre-Reproductive Period
topic_facet	Estuarine crabs Glyphosate Oocytes Ovarian growth Reproduction Anthropometry Estuaries Shellfish Tissue Active ingredients Estuarine crab Glyphosates Gonadosomatic index Herbicide glyphosate Oocytes Protein contents Reproduction Herbicides glyphosate neurohormone vitellogenin cell crab glyphosate growth reproduction weight adult animal experiment animal tissue Argentina Article assay body growth body weight gain controlled study crab estuarine species female hormone release in vitro study in vivo study Neohelice granulata nonhuman oocyte development ovary development protein content protein transport reproduction toxic concentration toxicity toxicity testing Argentina Decapoda (Crustacea)
description	Adult females of the estuarine crab Neohelice granulata were exposed during the 3-month pre-reproductive period (winter) to the herbicide glyphosate, the most used pesticide in Argentina, at three different concentrations (0.02, 0.2, and 1 mg/L, as active ingredient). At the end of the in vivo assay, the body weight gain and the ovarian growth were estimated, the last one in terms of the gonadosomatic index (GSI), the relative proportion of the different kind of oocytes, and their relative size. A decrease in the body weight gain was observed by effect of pure glyphosate, at all concentrations assayed. Although no differences in either the GSI or vitellogenic protein content of the ovary were noted between any glyphosate concentration and control, a higher proportion of reabsorbed vitellogenic oocytes was observed in the ovaries of crabs exposed to glyphosate at 1 mg/L, together with an increased area of previtellogenic oocytes. These effects were confirmed in vitro, at a glyphosate concentration of 0.2 mg/L. In fact, a higher area of previtellogenic oocytes was seen when glyphosate was added to the culture medium containing ovarian tissue, but a significant higher incidence of reabsorbed vitellogenic oocytes was seen only when eyestalk tissue was also added to the vials, suggesting that the secretion of some neurohormone involved in reabsorption is enhanced. The obtained results indicate that glyphosate is able to harm, in the studied species, both somatic and the ovarian growth. © 2018, Springer International Publishing AG, part of Springer Nature.
format	JOUR
author	Avigliano, L. Canosa, I.S. Medesani, D.A. Rodríguez, E.M.
author_facet	Avigliano, L. Canosa, I.S. Medesani, D.A. Rodríguez, E.M.
author_sort	Avigliano, L.
title	Effects of Glyphosate on Somatic and Ovarian Growth in the Estuarine Crab Neohelice granulata, During the Pre-Reproductive Period
title_short	Effects of Glyphosate on Somatic and Ovarian Growth in the Estuarine Crab Neohelice granulata, During the Pre-Reproductive Period
title_full	Effects of Glyphosate on Somatic and Ovarian Growth in the Estuarine Crab Neohelice granulata, During the Pre-Reproductive Period
title_fullStr	Effects of Glyphosate on Somatic and Ovarian Growth in the Estuarine Crab Neohelice granulata, During the Pre-Reproductive Period
title_full_unstemmed	Effects of Glyphosate on Somatic and Ovarian Growth in the Estuarine Crab Neohelice granulata, During the Pre-Reproductive Period
title_sort	effects of glyphosate on somatic and ovarian growth in the estuarine crab neohelice granulata, during the pre-reproductive period
url	http://hdl.handle.net/20.500.12110/paper_00496979_v229_n2_p_Avigliano
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_version_	1782030130926845952

Effects of Glyphosate on Somatic and Ovarian Growth in the Estuarine Crab Neohelice granulata, During the Pre-Reproductive Period

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