Induction of somatic growth in juvenile crayfish Cherax quadricarinatus (Decapoda, Parastacidae), by ecdysone and insulin growth factor

The effect of ecdysone and IGF-1 on the somatic growth of juvenile crayfish Cherax quadricarinatus, was examined both in vitro and in vivo. After 24. h of incubating explants of abdominal muscle, protein synthesis was augmented by exposure to 0.15 μM of ecdysone, 0.02 μM of IGF-1 or the combination...

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Autores principales: Chaulet, A., Medesani, D.A., Freitas, J., Cervino, A., Cervino, N., Rodríguez, E.M.
Formato: JOUR
Materias:
Crayfish
Ecdysone
Energetic reserves
IGF-1
Protein synthesis
Somatic growth
bioenergetics
crayfish
crustacean culture
food supplementation
growth regulator
hormone
juvenile
muscle
protein
Animalia
Astacoidea
Cherax quadricarinatus
Crustacea
Decapoda (Crustacea)
Parastacidae
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00448486_v370-371_n_p1_Chaulet
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_00448486_v370-371_n_p1_Chaulet
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spelling todo:paper_00448486_v370-371_n_p1_Chaulet2023-10-03T14:51:52Z Induction of somatic growth in juvenile crayfish Cherax quadricarinatus (Decapoda, Parastacidae), by ecdysone and insulin growth factor Chaulet, A. Medesani, D.A. Freitas, J. Cervino, A. Cervino, N. Rodríguez, E.M. Crayfish Ecdysone Energetic reserves IGF-1 Protein synthesis Somatic growth bioenergetics crayfish crustacean culture food supplementation growth regulator hormone juvenile muscle protein Animalia Astacoidea Cherax quadricarinatus Crustacea Decapoda (Crustacea) Parastacidae The effect of ecdysone and IGF-1 on the somatic growth of juvenile crayfish Cherax quadricarinatus, was examined both in vitro and in vivo. After 24. h of incubating explants of abdominal muscle, protein synthesis was augmented by exposure to 0.15 μM of ecdysone, 0.02 μM of IGF-1 or the combination of both hormones. The in vivo experiments were done by feeding the juveniles a pellet food supplemented with ecdysone, IGF-1 or a combination of both hormones, at doses equivalents to those that had an effect in the in vitro experiment. Animals fed IGF-1 or IGF-1 and ecdysone enriched pellets showed a higher weight gain than controls, while no differences were detected in the case of juveniles fed pellets enriched with ecdysone alone. In close correspondence with these results, and also with the in vitro results, a significant increment in protein content was found in muscle of crayfish treated with IGF-1, alone or in combination with ecdysone. On the other hand, significant lower lipid content was found in the hepatopancreas of both groups treated with IGF-1. Our results confirm the in vivo anabolic effects of IGF-1 on crustaceans, in terms of enhancing growth and protein synthesis, therefore identifying this hormone as a potential tool for crayfish culture. © 2012 Elsevier B.V. Fil:Medesani, D.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Rodríguez, E.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00448486_v370-371_n_p1_Chaulet
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Crayfish
Ecdysone
Energetic reserves
IGF-1
Protein synthesis
Somatic growth
bioenergetics
crayfish
crustacean culture
food supplementation
growth regulator
hormone
juvenile
muscle
protein
Animalia
Astacoidea
Cherax quadricarinatus
Crustacea
Decapoda (Crustacea)
Parastacidae
spellingShingle Crayfish
Ecdysone
Energetic reserves
IGF-1
Protein synthesis
Somatic growth
bioenergetics
crayfish
crustacean culture
food supplementation
growth regulator
hormone
juvenile
muscle
protein
Animalia
Astacoidea
Cherax quadricarinatus
Crustacea
Decapoda (Crustacea)
Parastacidae
Chaulet, A.
Medesani, D.A.
Freitas, J.
Cervino, A.
Cervino, N.
Rodríguez, E.M.
Induction of somatic growth in juvenile crayfish Cherax quadricarinatus (Decapoda, Parastacidae), by ecdysone and insulin growth factor
topic_facet Crayfish
Ecdysone
Energetic reserves
IGF-1
Protein synthesis
Somatic growth
bioenergetics
crayfish
crustacean culture
food supplementation
growth regulator
hormone
juvenile
muscle
protein
Animalia
Astacoidea
Cherax quadricarinatus
Crustacea
Decapoda (Crustacea)
Parastacidae
description The effect of ecdysone and IGF-1 on the somatic growth of juvenile crayfish Cherax quadricarinatus, was examined both in vitro and in vivo. After 24. h of incubating explants of abdominal muscle, protein synthesis was augmented by exposure to 0.15 μM of ecdysone, 0.02 μM of IGF-1 or the combination of both hormones. The in vivo experiments were done by feeding the juveniles a pellet food supplemented with ecdysone, IGF-1 or a combination of both hormones, at doses equivalents to those that had an effect in the in vitro experiment. Animals fed IGF-1 or IGF-1 and ecdysone enriched pellets showed a higher weight gain than controls, while no differences were detected in the case of juveniles fed pellets enriched with ecdysone alone. In close correspondence with these results, and also with the in vitro results, a significant increment in protein content was found in muscle of crayfish treated with IGF-1, alone or in combination with ecdysone. On the other hand, significant lower lipid content was found in the hepatopancreas of both groups treated with IGF-1. Our results confirm the in vivo anabolic effects of IGF-1 on crustaceans, in terms of enhancing growth and protein synthesis, therefore identifying this hormone as a potential tool for crayfish culture. © 2012 Elsevier B.V.
format JOUR
author Chaulet, A.
Medesani, D.A.
Freitas, J.
Cervino, A.
Cervino, N.
Rodríguez, E.M.
author_facet Chaulet, A.
Medesani, D.A.
Freitas, J.
Cervino, A.
Cervino, N.
Rodríguez, E.M.
author_sort Chaulet, A.
title Induction of somatic growth in juvenile crayfish Cherax quadricarinatus (Decapoda, Parastacidae), by ecdysone and insulin growth factor
title_short Induction of somatic growth in juvenile crayfish Cherax quadricarinatus (Decapoda, Parastacidae), by ecdysone and insulin growth factor
title_full Induction of somatic growth in juvenile crayfish Cherax quadricarinatus (Decapoda, Parastacidae), by ecdysone and insulin growth factor
title_fullStr Induction of somatic growth in juvenile crayfish Cherax quadricarinatus (Decapoda, Parastacidae), by ecdysone and insulin growth factor
title_full_unstemmed Induction of somatic growth in juvenile crayfish Cherax quadricarinatus (Decapoda, Parastacidae), by ecdysone and insulin growth factor
title_sort induction of somatic growth in juvenile crayfish cherax quadricarinatus (decapoda, parastacidae), by ecdysone and insulin growth factor
url http://hdl.handle.net/20.500.12110/paper_00448486_v370-371_n_p1_Chaulet
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