Ovarian growth in the crab Chasmagnathus granulata induced by hormones and neuroregulators throughout the year. In vivo and in vitro studies
The hormones 17α-hydroxyprogesterone and juvenile hormone III (JHIII), as well as the dopaminergic antagonist spiperone, were assayed both in vivo and in vitro on adult females of the estuarine crab Chasmagnathus granulata at different times of the year. All the chemicals were administered both by i...
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todo:paper_00448486_v224_n1-4_p339_Zapata2023-10-03T14:51:49Z Ovarian growth in the crab Chasmagnathus granulata induced by hormones and neuroregulators throughout the year. In vivo and in vitro studies Zapata, V. López Greco, L.S. Medesani, D. Rodríguez, E.M. Aquaculture Crustaceans Hormones Induction Neuroregulators Ovarian growth aquaculture crab hormone reproductive biology Chasmagnathus granulata Crustacea Decapoda (Crustacea) The hormones 17α-hydroxyprogesterone and juvenile hormone III (JHIII), as well as the dopaminergic antagonist spiperone, were assayed both in vivo and in vitro on adult females of the estuarine crab Chasmagnathus granulata at different times of the year. All the chemicals were administered both by injection and by incorporation into the food. Each of these in vivo assays lasted I month, and they were done during three different periods of the year, in accordance with the reproductive cycle of this crab (pre-, post- and reproductive periods). Spiperone and progesterone were always administered at a dose of 10-8 mol/crab, while JHIII at 10-7 mol/crab. The assays done during the reproductive period (spring and summer) yielded a significantly higher gonadosomatic index (GSI) than that of the concurrent control for all the chemicals assayed, whether injected or incorporated into the food. During the pre-reproductive period (winter), only progesterone and JHIII increased the GSI, while for the post-reproductive period (autumn), only spiperone and progesterone did so. These differences may be related to the phases of the annual reproductive cycle that is synchronized by both external and internal stimuli. In vitro assays that involved a 24-h incubation of isolated pieces of ovary from females sampled during the reproductive period were also done. A significant increase of leucine uptake by the ovary fragments was observed by merely adding either progesterone or JHIII to the incubation medium, but a similar effect of spiperone was only observed when thoracic ganglion (TG) was also added. Spiperone was also able to reverse the inhibitory effect caused by eyestalk tissue (ET) added to medium. The results of these in vitro assays support the hypothesis, previously suggested, about the inhibitory role of dopamine in crustacean reproduction acting on neuroendocrine organs such as TG and ET. © 2003 Elsevier Science B.V. All rights reserved. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00448486_v224_n1-4_p339_Zapata |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Aquaculture Crustaceans Hormones Induction Neuroregulators Ovarian growth aquaculture crab hormone reproductive biology Chasmagnathus granulata Crustacea Decapoda (Crustacea) |
| spellingShingle |
Aquaculture Crustaceans Hormones Induction Neuroregulators Ovarian growth aquaculture crab hormone reproductive biology Chasmagnathus granulata Crustacea Decapoda (Crustacea) Zapata, V. López Greco, L.S. Medesani, D. Rodríguez, E.M. Ovarian growth in the crab Chasmagnathus granulata induced by hormones and neuroregulators throughout the year. In vivo and in vitro studies |
| topic_facet |
Aquaculture Crustaceans Hormones Induction Neuroregulators Ovarian growth aquaculture crab hormone reproductive biology Chasmagnathus granulata Crustacea Decapoda (Crustacea) |
| description |
The hormones 17α-hydroxyprogesterone and juvenile hormone III (JHIII), as well as the dopaminergic antagonist spiperone, were assayed both in vivo and in vitro on adult females of the estuarine crab Chasmagnathus granulata at different times of the year. All the chemicals were administered both by injection and by incorporation into the food. Each of these in vivo assays lasted I month, and they were done during three different periods of the year, in accordance with the reproductive cycle of this crab (pre-, post- and reproductive periods). Spiperone and progesterone were always administered at a dose of 10-8 mol/crab, while JHIII at 10-7 mol/crab. The assays done during the reproductive period (spring and summer) yielded a significantly higher gonadosomatic index (GSI) than that of the concurrent control for all the chemicals assayed, whether injected or incorporated into the food. During the pre-reproductive period (winter), only progesterone and JHIII increased the GSI, while for the post-reproductive period (autumn), only spiperone and progesterone did so. These differences may be related to the phases of the annual reproductive cycle that is synchronized by both external and internal stimuli. In vitro assays that involved a 24-h incubation of isolated pieces of ovary from females sampled during the reproductive period were also done. A significant increase of leucine uptake by the ovary fragments was observed by merely adding either progesterone or JHIII to the incubation medium, but a similar effect of spiperone was only observed when thoracic ganglion (TG) was also added. Spiperone was also able to reverse the inhibitory effect caused by eyestalk tissue (ET) added to medium. The results of these in vitro assays support the hypothesis, previously suggested, about the inhibitory role of dopamine in crustacean reproduction acting on neuroendocrine organs such as TG and ET. © 2003 Elsevier Science B.V. All rights reserved. |
| format |
JOUR |
| author |
Zapata, V. López Greco, L.S. Medesani, D. Rodríguez, E.M. |
| author_facet |
Zapata, V. López Greco, L.S. Medesani, D. Rodríguez, E.M. |
| author_sort |
Zapata, V. |
| title |
Ovarian growth in the crab Chasmagnathus granulata induced by hormones and neuroregulators throughout the year. In vivo and in vitro studies |
| title_short |
Ovarian growth in the crab Chasmagnathus granulata induced by hormones and neuroregulators throughout the year. In vivo and in vitro studies |
| title_full |
Ovarian growth in the crab Chasmagnathus granulata induced by hormones and neuroregulators throughout the year. In vivo and in vitro studies |
| title_fullStr |
Ovarian growth in the crab Chasmagnathus granulata induced by hormones and neuroregulators throughout the year. In vivo and in vitro studies |
| title_full_unstemmed |
Ovarian growth in the crab Chasmagnathus granulata induced by hormones and neuroregulators throughout the year. In vivo and in vitro studies |
| title_sort |
ovarian growth in the crab chasmagnathus granulata induced by hormones and neuroregulators throughout the year. in vivo and in vitro studies |
| url |
http://hdl.handle.net/20.500.12110/paper_00448486_v224_n1-4_p339_Zapata |
| work_keys_str_mv |
AT zapatav ovariangrowthinthecrabchasmagnathusgranulatainducedbyhormonesandneuroregulatorsthroughouttheyearinvivoandinvitrostudies AT lopezgrecols ovariangrowthinthecrabchasmagnathusgranulatainducedbyhormonesandneuroregulatorsthroughouttheyearinvivoandinvitrostudies AT medesanid ovariangrowthinthecrabchasmagnathusgranulatainducedbyhormonesandneuroregulatorsthroughouttheyearinvivoandinvitrostudies AT rodriguezem ovariangrowthinthecrabchasmagnathusgranulatainducedbyhormonesandneuroregulatorsthroughouttheyearinvivoandinvitrostudies |
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1807318045206511616 |