Bacterial community shifts in nonylphenol polyethoxylates-enriched activated sludge
A molecular approach was used to evaluate the effect of nonylphenol ethoxylate surfactants on the bacterial diversity in lab-scale activated sludge reactors. Separate bench-scale units were fed synthetic wastewater with and without addition of branched nonylphenol ethoxylates (NPnEO). The performanc...
Guardado en:
Autores principales: | , , , , , , |
---|---|
Formato: | JOUR |
Materias: | |
Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_00431354_v38_n8_p2077_Lozada |
Aporte de: |
id |
todo:paper_00431354_v38_n8_p2077_Lozada |
---|---|
record_format |
dspace |
spelling |
todo:paper_00431354_v38_n8_p2077_Lozada2023-10-03T14:51:30Z Bacterial community shifts in nonylphenol polyethoxylates-enriched activated sludge Lozada, M. Itria, R.F. Figuerola, E.L.M. Babay, P.A. Gettar, R.T. De Tullio, L.A. Erijman, L. Activated sludge Betaproteobacteria Community structure Fluorescence in situ hybridization Nonionic surfactant Nonylphenol ethoxylates Bacteria Carbonaceous adsorbents Composition Degradation Probes Removal Solutions Surface active agents Wastewater Bacterial community Bench-scale units Feeding solutions Sludge reactors Sewage sludge 4',6 diamidino 2 phenylindole nonylphenol nonylphenol ethoxylate ribosome RNA surfactant unclassified drug activated sludge bacterium surfactant wastewater treatment article bacterial flora bacterial strain bacterium identification controlled study dot hybridization evaluation Gram negative bacterium in situ hybridization microbial diversity nonhuman oligonucleotide probe priority journal reactor separation technique sludge waste water management Aerobiosis Animals Bacteria Base Sequence Betaproteobacteria Biodegradation, Environmental Ethylene Glycols Fishes Fluorescent Dyes Gammaproteobacteria In Situ Hybridization Oligonucleotide Probes RNA, Ribosomal, 16S Sewage Soil Microbiology Surface-Active Agents Time Factors Bacteria (microorganisms) Betaproteobacteria Proteobacteria A molecular approach was used to evaluate the effect of nonylphenol ethoxylate surfactants on the bacterial diversity in lab-scale activated sludge reactors. Separate bench-scale units were fed synthetic wastewater with and without addition of branched nonylphenol ethoxylates (NPnEO). The performance of the reactors, in terms of carbonaceous removal was largely unaffected by the presence of NP10EO in the feeding solution. However, addition of NP10EO exerted a pronounced shift in bacterial community composition. In situ hybridization analyzing larger phylogenetic groups of bacteria with ribosomal RNA-targeted oligonucleotide probes revealed the dominance of clusters composed of Betaproteobacteria, accounting for up to one-third of 4′,6-diamidino-2- phenylindol-dihydrochloride (DAPI)-stained cells in NP10EO amended reactors and only 5% of DAPI-stained cells in the controls. These shifts in populations of larger phylogenetic groups were confirmed by dot-blot analysis of rRNA. Members of gamma subclass of Proteobacteria were present in low numbers in all activated sludge samples examined, suggesting that only bacteria affiliated with the beta subclass of Proteobacteria may have a specific role in NP10EO degradation. © 2004 Elsevier Ltd. All rights reserved. Fil:Itria, R.F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Figuerola, E.L.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Babay, P.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Erijman, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00431354_v38_n8_p2077_Lozada |
institution |
Universidad de Buenos Aires |
institution_str |
I-28 |
repository_str |
R-134 |
collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
topic |
Activated sludge Betaproteobacteria Community structure Fluorescence in situ hybridization Nonionic surfactant Nonylphenol ethoxylates Bacteria Carbonaceous adsorbents Composition Degradation Probes Removal Solutions Surface active agents Wastewater Bacterial community Bench-scale units Feeding solutions Sludge reactors Sewage sludge 4',6 diamidino 2 phenylindole nonylphenol nonylphenol ethoxylate ribosome RNA surfactant unclassified drug activated sludge bacterium surfactant wastewater treatment article bacterial flora bacterial strain bacterium identification controlled study dot hybridization evaluation Gram negative bacterium in situ hybridization microbial diversity nonhuman oligonucleotide probe priority journal reactor separation technique sludge waste water management Aerobiosis Animals Bacteria Base Sequence Betaproteobacteria Biodegradation, Environmental Ethylene Glycols Fishes Fluorescent Dyes Gammaproteobacteria In Situ Hybridization Oligonucleotide Probes RNA, Ribosomal, 16S Sewage Soil Microbiology Surface-Active Agents Time Factors Bacteria (microorganisms) Betaproteobacteria Proteobacteria |
spellingShingle |
Activated sludge Betaproteobacteria Community structure Fluorescence in situ hybridization Nonionic surfactant Nonylphenol ethoxylates Bacteria Carbonaceous adsorbents Composition Degradation Probes Removal Solutions Surface active agents Wastewater Bacterial community Bench-scale units Feeding solutions Sludge reactors Sewage sludge 4',6 diamidino 2 phenylindole nonylphenol nonylphenol ethoxylate ribosome RNA surfactant unclassified drug activated sludge bacterium surfactant wastewater treatment article bacterial flora bacterial strain bacterium identification controlled study dot hybridization evaluation Gram negative bacterium in situ hybridization microbial diversity nonhuman oligonucleotide probe priority journal reactor separation technique sludge waste water management Aerobiosis Animals Bacteria Base Sequence Betaproteobacteria Biodegradation, Environmental Ethylene Glycols Fishes Fluorescent Dyes Gammaproteobacteria In Situ Hybridization Oligonucleotide Probes RNA, Ribosomal, 16S Sewage Soil Microbiology Surface-Active Agents Time Factors Bacteria (microorganisms) Betaproteobacteria Proteobacteria Lozada, M. Itria, R.F. Figuerola, E.L.M. Babay, P.A. Gettar, R.T. De Tullio, L.A. Erijman, L. Bacterial community shifts in nonylphenol polyethoxylates-enriched activated sludge |
topic_facet |
Activated sludge Betaproteobacteria Community structure Fluorescence in situ hybridization Nonionic surfactant Nonylphenol ethoxylates Bacteria Carbonaceous adsorbents Composition Degradation Probes Removal Solutions Surface active agents Wastewater Bacterial community Bench-scale units Feeding solutions Sludge reactors Sewage sludge 4',6 diamidino 2 phenylindole nonylphenol nonylphenol ethoxylate ribosome RNA surfactant unclassified drug activated sludge bacterium surfactant wastewater treatment article bacterial flora bacterial strain bacterium identification controlled study dot hybridization evaluation Gram negative bacterium in situ hybridization microbial diversity nonhuman oligonucleotide probe priority journal reactor separation technique sludge waste water management Aerobiosis Animals Bacteria Base Sequence Betaproteobacteria Biodegradation, Environmental Ethylene Glycols Fishes Fluorescent Dyes Gammaproteobacteria In Situ Hybridization Oligonucleotide Probes RNA, Ribosomal, 16S Sewage Soil Microbiology Surface-Active Agents Time Factors Bacteria (microorganisms) Betaproteobacteria Proteobacteria |
description |
A molecular approach was used to evaluate the effect of nonylphenol ethoxylate surfactants on the bacterial diversity in lab-scale activated sludge reactors. Separate bench-scale units were fed synthetic wastewater with and without addition of branched nonylphenol ethoxylates (NPnEO). The performance of the reactors, in terms of carbonaceous removal was largely unaffected by the presence of NP10EO in the feeding solution. However, addition of NP10EO exerted a pronounced shift in bacterial community composition. In situ hybridization analyzing larger phylogenetic groups of bacteria with ribosomal RNA-targeted oligonucleotide probes revealed the dominance of clusters composed of Betaproteobacteria, accounting for up to one-third of 4′,6-diamidino-2- phenylindol-dihydrochloride (DAPI)-stained cells in NP10EO amended reactors and only 5% of DAPI-stained cells in the controls. These shifts in populations of larger phylogenetic groups were confirmed by dot-blot analysis of rRNA. Members of gamma subclass of Proteobacteria were present in low numbers in all activated sludge samples examined, suggesting that only bacteria affiliated with the beta subclass of Proteobacteria may have a specific role in NP10EO degradation. © 2004 Elsevier Ltd. All rights reserved. |
format |
JOUR |
author |
Lozada, M. Itria, R.F. Figuerola, E.L.M. Babay, P.A. Gettar, R.T. De Tullio, L.A. Erijman, L. |
author_facet |
Lozada, M. Itria, R.F. Figuerola, E.L.M. Babay, P.A. Gettar, R.T. De Tullio, L.A. Erijman, L. |
author_sort |
Lozada, M. |
title |
Bacterial community shifts in nonylphenol polyethoxylates-enriched activated sludge |
title_short |
Bacterial community shifts in nonylphenol polyethoxylates-enriched activated sludge |
title_full |
Bacterial community shifts in nonylphenol polyethoxylates-enriched activated sludge |
title_fullStr |
Bacterial community shifts in nonylphenol polyethoxylates-enriched activated sludge |
title_full_unstemmed |
Bacterial community shifts in nonylphenol polyethoxylates-enriched activated sludge |
title_sort |
bacterial community shifts in nonylphenol polyethoxylates-enriched activated sludge |
url |
http://hdl.handle.net/20.500.12110/paper_00431354_v38_n8_p2077_Lozada |
work_keys_str_mv |
AT lozadam bacterialcommunityshiftsinnonylphenolpolyethoxylatesenrichedactivatedsludge AT itriarf bacterialcommunityshiftsinnonylphenolpolyethoxylatesenrichedactivatedsludge AT figuerolaelm bacterialcommunityshiftsinnonylphenolpolyethoxylatesenrichedactivatedsludge AT babaypa bacterialcommunityshiftsinnonylphenolpolyethoxylatesenrichedactivatedsludge AT gettarrt bacterialcommunityshiftsinnonylphenolpolyethoxylatesenrichedactivatedsludge AT detulliola bacterialcommunityshiftsinnonylphenolpolyethoxylatesenrichedactivatedsludge AT erijmanl bacterialcommunityshiftsinnonylphenolpolyethoxylatesenrichedactivatedsludge |
_version_ |
1782028857940901888 |