Response of cells persistently infected with arenaviruses to superinfection with homotypic and heterotypic viruses

Vero cell cultures persistently infected with the arenaviruses Junin, Pichinde, Tacaribe, and Tamiami were established and designated Vero-Jun, Vero-Pic, Vero-Tac, and Vero-Tam, respectively. Two types of carrier cultures could be easily distinguished: Vero-Jun and Vero-Tac systems were characterize...

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Autores principales: Damonte, E.B., Mersich, S.E., Coto, C.E.
Formato: JOUR
Materias:
arenavirus
cell culture
in vitro study
nonhuman
persistent infection
virus infection
virus interference
Animal
Arenaviridae
Arenaviruses, New World
Cell Line
Cercopithecus aethiops
Comparative Study
Plaque Assay
Support, Non-U.S. Gov't
Ultraviolet Rays
Viral Interference
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00426822_v129_n2_p474_Damonte
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_00426822_v129_n2_p474_Damonte
record_format dspace
spelling todo:paper_00426822_v129_n2_p474_Damonte2023-10-03T14:51:24Z Response of cells persistently infected with arenaviruses to superinfection with homotypic and heterotypic viruses Damonte, E.B. Mersich, S.E. Coto, C.E. arenavirus cell culture in vitro study nonhuman persistent infection virus infection virus interference Animal Arenaviridae Arenaviruses, New World Cell Line Cercopithecus aethiops Comparative Study Plaque Assay Support, Non-U.S. Gov't Ultraviolet Rays Viral Interference Vero cell cultures persistently infected with the arenaviruses Junin, Pichinde, Tacaribe, and Tamiami were established and designated Vero-Jun, Vero-Pic, Vero-Tac, and Vero-Tam, respectively. Two types of carrier cultures could be easily distinguished: Vero-Jun and Vero-Tac systems were characterized by a lack of infectious virus production after a few cell transfers, whereas a more productive state with continuous release of virus was observed in Vero-Pic and Vero-Tam cultures. These differences appeared to be related to resistance of the culture to viral superinfection. In fact, Vero-Jun and Vero-Tac cultures totally excluded only the replication of the serologically more closely related arenaviruses Amapari, Junin, or Tacaribe, while the refractoriness of Vero-Pic and Vero-Tam cultures was extended to most of the virus group members. The resistance of Vero-Jun cells to superinfection by Junin or Tacaribe virus could be ascribed to the production of specific uv-resistant Junin interfering particles, which showed a specific range of interference against Junin and Tacaribe viruses. Interfering particles against homotypic and heterotypic arenaviruses were isolated from Vero-Pic cultures. However, the degree of interference developed by these Pic-interfering particles was not enough to fully explain reinfecting virus exclusion from Vero-Pic cultures. Viral susceptibility of persistent cultures is proposed as a useful tool to examine relationships of members of the arenavirus group. © 1983. Fil:Damonte, E.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Mersich, S.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Coto, C.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00426822_v129_n2_p474_Damonte
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic arenavirus
cell culture
in vitro study
nonhuman
persistent infection
virus infection
virus interference
Animal
Arenaviridae
Arenaviruses, New World
Cell Line
Cercopithecus aethiops
Comparative Study
Plaque Assay
Support, Non-U.S. Gov't
Ultraviolet Rays
Viral Interference
spellingShingle arenavirus
cell culture
in vitro study
nonhuman
persistent infection
virus infection
virus interference
Animal
Arenaviridae
Arenaviruses, New World
Cell Line
Cercopithecus aethiops
Comparative Study
Plaque Assay
Support, Non-U.S. Gov't
Ultraviolet Rays
Viral Interference
Damonte, E.B.
Mersich, S.E.
Coto, C.E.
Response of cells persistently infected with arenaviruses to superinfection with homotypic and heterotypic viruses
topic_facet arenavirus
cell culture
in vitro study
nonhuman
persistent infection
virus infection
virus interference
Animal
Arenaviridae
Arenaviruses, New World
Cell Line
Cercopithecus aethiops
Comparative Study
Plaque Assay
Support, Non-U.S. Gov't
Ultraviolet Rays
Viral Interference
description Vero cell cultures persistently infected with the arenaviruses Junin, Pichinde, Tacaribe, and Tamiami were established and designated Vero-Jun, Vero-Pic, Vero-Tac, and Vero-Tam, respectively. Two types of carrier cultures could be easily distinguished: Vero-Jun and Vero-Tac systems were characterized by a lack of infectious virus production after a few cell transfers, whereas a more productive state with continuous release of virus was observed in Vero-Pic and Vero-Tam cultures. These differences appeared to be related to resistance of the culture to viral superinfection. In fact, Vero-Jun and Vero-Tac cultures totally excluded only the replication of the serologically more closely related arenaviruses Amapari, Junin, or Tacaribe, while the refractoriness of Vero-Pic and Vero-Tam cultures was extended to most of the virus group members. The resistance of Vero-Jun cells to superinfection by Junin or Tacaribe virus could be ascribed to the production of specific uv-resistant Junin interfering particles, which showed a specific range of interference against Junin and Tacaribe viruses. Interfering particles against homotypic and heterotypic arenaviruses were isolated from Vero-Pic cultures. However, the degree of interference developed by these Pic-interfering particles was not enough to fully explain reinfecting virus exclusion from Vero-Pic cultures. Viral susceptibility of persistent cultures is proposed as a useful tool to examine relationships of members of the arenavirus group. © 1983.
format JOUR
author Damonte, E.B.
Mersich, S.E.
Coto, C.E.
author_facet Damonte, E.B.
Mersich, S.E.
Coto, C.E.
author_sort Damonte, E.B.
title Response of cells persistently infected with arenaviruses to superinfection with homotypic and heterotypic viruses
title_short Response of cells persistently infected with arenaviruses to superinfection with homotypic and heterotypic viruses
title_full Response of cells persistently infected with arenaviruses to superinfection with homotypic and heterotypic viruses
title_fullStr Response of cells persistently infected with arenaviruses to superinfection with homotypic and heterotypic viruses
title_full_unstemmed Response of cells persistently infected with arenaviruses to superinfection with homotypic and heterotypic viruses
title_sort response of cells persistently infected with arenaviruses to superinfection with homotypic and heterotypic viruses
url http://hdl.handle.net/20.500.12110/paper_00426822_v129_n2_p474_Damonte
work_keys_str_mv AT damonteeb responseofcellspersistentlyinfectedwitharenavirusestosuperinfectionwithhomotypicandheterotypicviruses
AT mersichse responseofcellspersistentlyinfectedwitharenavirusestosuperinfectionwithhomotypicandheterotypicviruses
AT cotoce responseofcellspersistentlyinfectedwitharenavirusestosuperinfectionwithhomotypicandheterotypicviruses
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